Searchable abstracts of presentations at key conferences in endocrinology

ea0019p224 | Pituitary | SFEBES2009

Cloning and expression of calcium/calmodulin-dependent protein kinase ii isoforms in the developing zebrafish (Danio rerio)

Chand A , McGonnell I , Fowkes R

The family of calcium/calmodulin-dependent protein kinases (CaMK) are major effectors of calcium binding proteins in virtually all systems. Recently, CaMKs have been implicated in regulating pituitary and hypothalamic gene transcription to control the expression of gonadotrophin hormones and adrenocorticotrophin in the pituitary, and as a pivotal protein in the satiety pathway. Despite these observations, the spatial and temporal expression of the CaMKs is poorly documented an...

ea0015p262 | Pituitary | SFEBES2008

Transcriptional regulation of the human guanylyl cyclase-B (GC-B) promoter in gonadotroph and somatotroph cell lines

Chand Annisa , McGonnell Imelda , Fowkes Rob

Particulate guanylyl cyclases are expressed in all endocrine organs and serve as specific receptors of the natriuretic peptides ANP, BNP and CNP. Guanylyl cyclase-B (GC-B/Npr2) receptors specifically mediate the biological effects of CNP in its target tissues. Targeted disruption of either CNP or GC-B results in pituitary growth hormone deficiency and dwarfism as a result of achondroplasia. Despite this, molecular regulation of GC-B expression is poorly elucidated. Therefore, ...

ea0012oc20 | Pituitary, ovary and steroids | SFE2006

Novel activation of MAPK family proteins in pituitary GH3 somatotrophs and alphaT3-1 gonadotrophs by C-type natriuretic peptide (CNP)

Jonas KC , Thompson IA , Chand AN , Fowkes RC

In target tissues, CNP acts via the cell surface guanylyl cyclase B (GC-B) receptor to stimulate cGMP accumulation. Transgenic mice studies deleting either CNP or the GC-B receptor suggest a role for CNP in regulating bone formation and pituitary growth hormone secretion. Our previous studies have implicated a role for CNP in inhibiting calcium transients in alphaT3-1 pituitary gonadotroph cells; however the biological consequence of cGMP accumulation remains unclear. Therefor...

ea0013p245 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Phosphorylation of MAPK family proteins by C-type natriuretic peptide (CNP) in LbetaT2 gonadotrophs – a novel cyclic GMP-independent effect?

Jonas Kim , Thompson Iain , Chand Annisa , Fowkes Rob

CNP, the third member of the mammalian natriuretic peptide family, exerts the majority of its biological effects via the guanylyl cyclase B (GC-B) receptor, resulting in accumulation of intracellular cGMP. Previous studies reveal numerous inhibitory effects of natriuretic peptides on the MAPK family in bone, neuronal and cardiovascular tissues. However, we recently reported that CNP stimulates ERK, p38MAPK and JNK phosphorylation in pituitary GH3 somatotrophs. As CNP is known ...

ea0010oc16 | Thyroid and pituitary | SFE2005

Expression and function of NR5A1, NR5A2 and NR0B1 in mediating gonadotrophin-releasing hormone (GNRH) function in gonadotroph cell lines

Mirczuk S , Poulet B , Chand A , Burrin|R##Fowkes J

The nuclear receptors, NR5A1 (SF-1), NR5A2 (LRH-1) and NR0B1 (DAX-1), are key regulators of gene expression and development in endocrine tissues. NR5A1 regulates endocrine development and function, and is an important regulator of gene expression in pituitary gonadotrophs. In contrast, little is known regarding the role of NR5A2 in pituitary function. Using a range of anterior pituitary-derived cell lines and primary rat pituitaries, we have investigated the expression and act...

ea0041ep84 | Adrenal medulla | ECE2016

Pheochromocytoma/paraganglioma: Histopathological features as clue to the underlying germline mutation in these genetically heterogeneous tumors

Roy Maitrayee , Agarwal Shipra , Sharma Mehar Chand , Gupta Poonam , Khurana M L , Khagawat Rajesh , Tandon Nikhil

Introduction: Pheochromocytomas (PHEOs) and paragangliomas (PGLs) are rare neuroendocrine tumors arising from chromaffin cells within adrenal medulla and autonomic paragranglia respectively. Recent evidences show that nearly one-third patients harbour germline mutation, namely in von Hippel-Lindau (VHL), REarranged during Transfection (RET), neurofibromatosis type 1 (NF 1) and succinate dehydrogenase (SDH) complex genes. However, the tumor morphology arising in various syndrom...

ea0041ep1044 | Thyroid (non-cancer) | ECE2016

Technique of story-telling in patients undergoing thyroid surgery

Mayilvaganan Sabaretnam , Chand Gyan , Mishra Saroj , Agarwal Amit , Agarwal Gaurav , mishra Anjali , Verma AK , Warsi Dabeer

Introduction: In the modern day busy clinical practice the communication between patient and care giver is at minimal level. The patients feel apprehensive when advised about the surgical intervention. In such situation they feel the need of detail information from the care givers.Story telling is a tool which can be used for this problem. We intended to study the efficacy of storytelling technique on patients undergoing Hemithyroidectomy for benign cytology.<p class="abst...

ea0031p45 | Clinical biochemistry | SFEBES2013

Persistent hyperparathyroidism following parathyroidectomy: can routine vitamin D replacement prior to surgery alter post-operative secondary hyperparathyroidism?

Chand Natalie , Weston-Petrides Gina , Evans Abigail , Skene Anthony , Begley Joe , Antonas Philipp , Richardson Tristan

Introduction: A persistently elevated parathyroid hormone (PTH) following parathyroidectomy is usually associated with vitamin D deficiency. We have previously demonstrated this to occur in ~60% of post-operative patients. We have examined the effect of routine vitamin D replacement pre-operatively on the proportion of patients with persistently elevated PTH post-operatively.Methods: Data was collated retrospectively from our parathyroid database, includ...

ea0021p291 | Pituitary | SFEBES2009

Fish and chicks: C-type natriuretic peptide and the development of the pituitary gland in Gallus gallus and Danio rerio

Chand Annisa , Akbareian Sophia , McSloy Alex , Renshaw Derek , McGonnell Imelda , Fowkes Robert

Mammalian pituitary gland development is well established on a molecular and morphological level. In developmental biology the use of chick and zebrafish models is common but despite chicken pituitary gland development being characterised in 1952 these models have only recently been regularly employed in pituitary studies. The third member of the natriuretic peptide family, C-type natriuretic peptide (CNP), has been investigated in rodent embryos but little is known about its ...

ea0015s33 | Evolving endocrine targets for C-type natriuretic peptide (CNP) | SFEBES2008

The new biology of pituitary natriuretic peptides: novel signalling from guanylyl cyclase-B receptors

Fowkes Rob , Jonas Kim , Thompson Iain , Chand Annisa , Ansorge Olaf , Wass John

Despite being identified almost two decades ago, the biological roles for C-type natriuretic peptide (CNP) have remained poorly elucidated. Both CNP and GC-B are highly conserved from flies and fish to humans. Recent investigations involving the disruption of the CNP gene (Nppc) or that of its GC-B receptor (Npr2) implicate a role for CNP and GC-B in bone formation and growth. Intriguingly, CNP and GC-B knock-out mouse models also exhibited pituitary growth hormone deficiency....