Searchable abstracts of presentations at key conferences in endocrinology

ea0059p037 | Adrenal and steroids | SFEBES2018

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

Khan Shazia , Cobice Diego F , Livingstone Dawn EW , Mackay C Logan , Webster Scott P , Walker Brian R , Andrew Ruth

Excess action of glucocorticoid hormones is implicated in metabolic disease and cognitive decline, 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) catalysing generation of active glucocorticoid hormones in tissues. The penetration rates and tissue-specific contribution of 11βHSD1 to glucocorticoid turnover were assessed using tracer kinetics. 9,11,12,12-d4-Cortisol (d4F) was infused (1.75 mg/day, 7 days) into C57Bl6/J male mice and mice lacking 11βHSD1 (n<...

ea0034p249 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2014

Derivatisation of estrogens enhances specificity and sensitivity of analysis by liquid chromatography tandem mass spectrometry

Faqehi Abdullah M M , Cobice Diego F , Naredo Gregorio , Mak Tracy C S , Walker Brian R , Homer Natalie Z M , Andrew Ruth

Physiological circulating concentrations of estrogens are very low in men and postmenopausal women (<50 pg/ml), which presenting analytical challenges. Immunoassays can detect as low as 30 pg/ml, but cross-reactivity is a concern. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) offers greater specificity than immunoassays, but ionisation of estrogens is inefficient. Derivatisation, which introduces charged moieties, may enhance ionisation. Dansylated de...