Abstract Search

ea0059p037 | Adrenal and steroids | SFEBES2018

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

Khan Shazia , Cobice Diego F , Livingstone Dawn EW , Mackay C Logan , Webster Scott P , Walker Brian R , Andrew Ruth

Excess action of glucocorticoid hormones is implicated in metabolic disease and cognitive decline, 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) catalysing generation of active glucocorticoid hormones in tissues. The penetration rates and tissue-specific contribution of 11βHSD1 to glucocorticoid turnover were assessed using tracer kinetics. 9,11,12,12-d4-Cortisol (d4F) was infused (1.75 mg/day, 7 days) into C57Bl6/J male mice and mice lacking 11βHSD1 (n<...

0 shares

ea0034p249 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2014

Derivatisation of estrogens enhances specificity and sensitivity of analysis by liquid chromatography tandem mass spectrometry

Faqehi Abdullah M M , Cobice Diego F , Naredo Gregorio , Mak Tracy C S , Walker Brian R , Homer Natalie Z M , Andrew Ruth

Physiological circulating concentrations of estrogens are very low in men and postmenopausal women (<50 pg/ml), which presenting analytical challenges. Immunoassays can detect as low as 30 pg/ml, but cross-reactivity is a concern. Liquid chromatographytandem mass spectrometry (LCMS/MS) offers greater specificity than immunoassays, but ionisation of estrogens is inefficient. Derivatisation, which introduces charged moieties, may enhance ionisation. Dansylated de...

0 shares

ePoster

Endocrine Abstracts

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

Derivatisation of estrogens enhances specificity and sensitivity of analysis by liquid chromatography tandem mass spectrometry

BiosciAbstracts