Searchable abstracts of presentations at key conferences in endocrinology

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0022p694 | Obesity | ECE2010

Reference interval values of four plasmatic endocannabinoids measured by isotopic dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)

Fanelli Flaminia , Cuomo Gaia , Di Lallo Valentina Diana , Belluomo Ilaria , Baccini Margherita , Gambineri Alessandra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

The endocannabinoid system is involved in modulating many metabolic and behavioural processes. Due to the instable nature of endocannabinoids in biological fluids and to the poor sensitivity of traditional analytical technologies, little is known about physiological circulating levels of these lipid mediators. We developed and validated an ID-LC-MS/MS method for the simultaneous measurement of four endocannabinoids: anandamide (AEA), 2-arachidonoylglycerol (2-AG), palmitoyl-et...

ea0022p740 | Steroid metabolism & action | ECE2010

Simultaneous steroid measurement by isotopic dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS): comparison with routine analysis methods and reference intervals in normal subjects

Fanelli Flaminia , Belluomo Ilaria , Di Lallo Valentina Diana , Cuomo Gaia , Baccini Margherita , Vicennati Valentina , Gambineri Alessandra , Pasquali Renato , Pagotto Uberto

The development of a reliable and rapid method to simultaneously measure serum steroids represents a compelling challenge for modern endocrinology. Immunoassays are very sensitive methods, being widely employed in automated routine laboratories, lacking, however, in specificity due to cross-reactivity and matrix interference. Gas chromatography–mass spectrometry (GC-MS) constitutes the reference method for quantitative analysis of steroids, but time-consuming sample pre-t...

ea0026p74 | Endocrine tumours and neoplasia | ECE2011

In vitro effect of the somatostatin analogue BIM 23704 on cell cycle timecourse in an ovarian carcinoma cell line

de Angelis Cristina , Pivonello Claudia , Cuomo Gaia , Sardo Attilio di Spiezio , Bifulco Guiseppe , Nappi Carmine , Sabbatino Francesco , Cariati Federica , Colao Annamaria , Pivonello Rosario

Ovarian carcinoma (OC) is associated with poor prognosis and low survival rate. Eligible treatment for advanced OC is surgery followed by chemotherapy based on a combination of Platinum and Taxol, but unfortunately long-term outcome of such therapy is disappointing. Moreover, systemic administration of cytotoxic agents is usually accompanied by a series of side effects. On the basis of this evidence, more selective and tumour cell-targeted therapeutic approaches are warranted....