Searchable abstracts of presentations at key conferences in endocrinology

ea0073pep12.7 | Presented ePosters 12: Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Systems biology approach identifies key genes and related-pathways for childhood obesity

Crispim Daisy , Pellenz Felipe , Assmann Tais

IntroductionEpidemiological studies have suggested that obesity during childhood increases the risk of developing metabolic comorbidities, such as type 2 diabetes, later in life. Childhood obesity is a complex disease whose molecular mechanisms are not completely elucidated. However, studies have demonstrated that approximately 65% of the variation associated with obesity is due to genetic factors. In this context, a system biology approach could contrib...

ea0063gp121 | Diabetes: Late Complications | ECE2019

The A allele of the rs4636297 (G/A) polymorphism in the microRNA-126 is associated with protection for diabetic retinopathy

Crispim Daisy , Massignam Eloisa , Pellenz Felipe Mateus , Dieter Cristine

Background and aims: Diabetic retinopathy (DR) is an important chronic complication of diabetes mellitus (DM), and is the leading cause of new cases of blindness in adults. Although several single nucleotide polymorphisms (SNPs) have been associated with DR, more information is needed to unravel the genetics of this complex disease. MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate expression of at least 60% of all protein-coding genes. A number of miRNAs ha...

ea0049gp90 | Diabetes & complications 2 | ECE2017

The G allele of the BDKRB1 rs12050217 polymorphism is associated with protection for diabetic retinopathy

Brondani Leticia A. , Pisco Julia , Guimaraes Jorge A. , Crispim Daisy , Berger Markus

Background: Diabetic retinopathy (DR) is a chronic diabetic complication occurring in most diabetic patients after 20 years of disease. Progression of DR to its sight threatening stages is usually associated with worsening of underlying retinal vascular dysfunction and disease. The plasma kallikrein kinin system is activated during vascular injury, mediating important functions in inflammation, blood flow, and coagulation. Bradykinin B1 receptor (B1R) is expressed in human ret...

ea0063gp135 | Obesity | ECE2019

Obesity is associated with a downregulation of UCP2 and miR-133a-3p but not NLRP3 in subcutaneous adipose tissue

Souza Bianca de , Oliveira Mayara de , Rheinheimer Jakeline , Moehlecke Milene , Rodrigues Michelle , Leitao Cristiane , Crispim Daisy

Background and aims: Obesity is a chronic multifactorial disease characterized by an excessive accumulation of body fat resulting from an imbalance between energy intake and caloric expenditure. Uncoupling protein 2 (UCP2) is located in the inner mitochondrial membrane and plays an important role in the regulation of energy expenditure. NLR family, pyrin domain-containing 3 (NLRP3) inflammasome seems to have a key role in obesity-induced inflammation through production of the ...

ea0063gp217 | Gestational and Type 1 Diabetes | ECE2019

The rs2304256 A allele in the TYK2 gene is associated with protection for type 1 diabetes mellitus in a Brazilian population

Crispim Daisy , Pellenz Felipe Mateus , Duarte Guilherme , Dieter Cristine , Souza Bianca de

Background and Aims: Type 1 diabetes mellitus (T1DM) is caused by an autoimmune destruction of pancreatic beta cells, which renders patients insulin-dependent for life. The disease arises from a complex interaction among several genetic and environmental factors. To date, single nucleotide polymorphisms (SNPs) in >50 genes have been associated with T1DM, with HLA class II SNPs having the greatest impact on disease susceptibility. Other loci have minor effects on T...

ea0049oc2.1 | Diabetes Prediction and Complications | ECE2017

microRNA expression profile in plasma from patients with type 1 diabetes: a case–control study and bioinformatics analysis

Assmann Tais Silveira , Recamonde-Mendoza Mariana , Punales Marcia , Tschiedel Balduino , Canani Luis Henrique , Crispim Daisy

Context: Since the exact cause of type 1 diabetes mellitus (T1DM) remains unclear, the detection of novel biomarkers is necessary to complement the information obtained from the presence of autoantibodies together with genetic and environmental risk factors. MicroRNAs (miRNAs) are a class of small noncoding RNA molecules that negatively regulate gene expression. Changes in their expression were described in several pathological conditions, including autoimmune diseases. Circul...

ea0049gp79 | Diabetes & complications 1 | ECE2017

The rs2292239 polymorphism in the ERBB3 gene is associated with risk for type 1 diabetes mellitus

Crispim Daisy , Dieter Cristine , Lemos Natalia , Assmann Tais , Duarte Guilherme , Dorfman Luiza , Bauer Andrea

Background and Aim: Type 1 diabetes mellitus (T1DM) is an autoimmune disease resulting from the complex interaction among multiple genes, environmental factors and the immune system. Genome-wide association studies identified ERBB3 (Erb-b2 receptor tyrosine kinase 3) gene as a main susceptibility locus for T1DM. This gene encodes a member of the family of intracellular receptors of protein tyrosine kinases, which activates multiple signaling pathways including PI3K-Ak...

ea0049gp93 | Diabetes therapy & complications 1 | ECE2017

microRNA expression profile in plasma from patients with diabetic kidney disease

Assmann Tais Silveira , Punales Marcia , Tschiedel Balduino , Canani Luis Henrique , Bauer Andrea Carla , Crispim Daisy

Introduction: Diabetic kidney disease (DKD) is the major cause of end-stage renal disease; however, the pathogenesis of this disease is not fully understood. Currently available therapies are not totally efficacious in the treatment and prognosis of DKD, suggesting that further understanding of the molecular mechanisms underlying the pathogenesis of this disease is necessary to improve its management. Recently, research on microRNAs (miRNAs) has become a hotspot because of the...

ea0049ep411 | Diabetes (to include epidemiology, pathophysiology) | ECE2017

GLIS3 rs7020673 and rs10758593 polymorphisms interact in the susceptibility for type 1 diabetes mellitus

Crispim Daisy , Duarte Guilherme , Assmann Tais , Dieter Cristine , de Souza Bianca , Canani Luis

Background and Aims: The transcription factor Gli-similar 3 (GLIS3) plays a key role in the development and maintenance of pancreatic beta-cells as well as in the regulation of Insulin gene expression in adults. Accordingly, genome-wide association studies identified GLIS3 as a susceptibility locus for type 1 diabetes mellitus (T1DM) and glucose metabolism traits. Therefore, the aim of this study was to investigate the association of the rs7020673 (G/C) and r...

ea0049ep648 | Obesity | ECE2017

CGI-58 gene expression profile in individuals with different body mass index

Crispim Daisy , Rheinheimer Jakeline , Moehlecke Milene , Souza Bianca de , Leitao Cristiane

Background and aim: Although chronic low-grade inflammation is observed in obese subjects, the underlying mechanisms modulating this process are still unclear. The Comparative Gene Identification-58 (CGI-58) is a lipid droplet-associated protein that has an important role in mediating intracellular fat hydrolysis by acting as a coactivator of the adipose Triglyceride Lipase (ATGL). Recent evidence suggests that CGI-58 also has a role in chronic inflammation and insulin resista...