ea0029s26.2 | TSH Receptor | ICEECE2012
Davies Terry F.
, Morshed Syed.
, Latif Rauf.
Successful crystallization of TSHR residues 21260 has been reported (Sanders et al. Thyroid 2007 17 395) using a partial ectodomain (ECD) bound to the Fab fragment of a human stimulating TSHR monoclonal antibody (M22) and its conformational epitopes delineated. We have now studied antibody binding to the entire ECD (residues 1412) using epitope protection. In this approach, we first protected a highly purified ectodomain fragment with a variety...