Searchable abstracts of presentations at key conferences in endocrinology

ea0057001 | tRNAGln hypomethylation and fragmentation in patient iPSC-derived β-like cells mediates apoptosis in TRMT10A diabetes | BES2018

tRNAGln hypomethylation and fragmentation in patient iPSC-derived β-like cells mediates apoptosis in TRMT10A diabetes

Cristina Cosentino , Sanna Toivonen , Stephane Demine , Andrea Schiavo , Nathalie Pachera , Decio L Eizirik , Miriam Cnop , Mariana Igoillo-Esteve

Background and aim: Loss-of-function mutations in TRMT10A, a transfer RNA (tRNA) methyltransferase, cause early onset diabetes and microcephaly. tRNAs play a crucial role in cellular homeostasis and post-transcriptional modifications modulate tRNA function and fragmentation. tRNA-derived halves (tiRNAs, 29-50 nt) and fragments (tRFs, 14-30 nt) are a new class of functional small noncoding RNAs, involved in cellular stress responses. Here we set out to investigate the ...

ea0088001 | Abstracts | BES2022

Exercise as a non-pharmacological intervention to protect pancreatic beta cells in patients with type 1 and type 2 diabetes

de Brachene Alexandra Coomans , Corentin Scoubeau , Anyishai E Musuaya , Angela Castela , Julie Carpentier , Vitalie Faoro , Malgorzata Klass , Miriam Cnop , Decio L Eizirik

Aim of the work: Diabetes is characterized by progressive loss of functional pancreatic beta cells. None of the therapeutic agents used to treat diabetes arrest this process and preventing beta cell loss remains a major unmet need. We have previously shown that serum from 8 young healthy males who exercised for 8 weeks protects human islets and human insulin-producing EndoC-βH1 cells from apoptosis induced by pro-inflammatory cytokines or the endoplasmic reticulum (ER) st...

ea0088007 | Abstracts | BES2022

The impact of interferon-α on global gene expression in iPSC-derived β- and α-like cells

Sandra Marin-Canas , Florian Szymczak , Maria Ines Alvelos , Stephane Demine , Maikel L Colli , de Beeck Anne Op , Sofia Thomaidou , Lorella Marselli , Arnaud Zaldumbide , Piero Marchetti , Decio L Eizirik

Aim: IFNα is a key regulator of the initial dialogue between pancreatic β-cells and the immune system in type 1 diabetes (T1D). IFNα induces endoplasmic reticulum (ER) stress, insulitis and a massive HLA-ABC overexpression in human β-cells, three histological hallmarks of T1D. Against this background we investigated the global role of IFNα on iPSC-derived islet-like cells, used here to mimic islet cells in the early neonatal period when autoimmunity ag...