Searchable abstracts of presentations at key conferences in endocrinology

ea0081yi4 | Young Investigator Awards | ECE2022

The role of miR-335-5p in the differentiation of thyroid cancers with BRAF mutation

Pecce Valeria , Verrienti Antonella , Sponziello Marialuisa , Grani Giorgio , Bini Simone , Filetti Sebastiano , Durante Cosimo

The most frequent mutation in papillary thyroid carcinoma (PTC) is the p.V600E of the BRAF gene. This mutation leads to the aberrant activation of the RAS / BRAF / MEK / ERK pathway and consequently to the under-regulation of thyroid-specific genes, resulting in uncontrolled growth and de-differentiation of cancer cells. In this work, we analyzed the transcriptomics data produced by the TCGA project using a network approach. The analysis led to the identification of r...

ea0070aep1062 | Hot topics (including COVID-19) | ECE2020

The emotional outbreak of (endocrine) cancer patients during COVID-19 pandemic

Falcone Rosa , Grani Giorgio , Ramundo Valeria , Melcarne Rossella , Giacomelli Laura , Filetti Sebastiano , Durante Cosimo

Background: The 2019 coronavirus disease (COVID-19) pandemic is a public health emergency and raises the issue of psychological and physical resilience in more vulnerable patients. We investigated the emotional impact of the SARS-CoV-2 spread among cancer patients, followed at our Institution in Italy, after the restrictive measures imposed by the government and the disruption of planned clinical activities.Methods: From March 18th to April 1st, 2020, we...

ea0081oc3.5 | Oral Communications 3: Thyroid 1 | ECE2022

Functional analysis of exosomal miRNAs secreted by thyroid cancer cells

Verrienti Antonella , Maggisano Valentina , Capriglione Fancesca , Sponziello Marialuisa , Pecce Valeria , Durante Cosimo , Celano Marilena , Bulotta Stefania , Russo Diego

Background: MiRNAs transported by exosomes play a role in various processes of tumorigenesis and tumor progression. They can function as oncogenes able to structurally and biologically change the tumor microenvironment (TME) therefore playing a crucial role in cell-to-cell communication during tumor development and allowing cancer cells to become invasive and disseminate from the primary site to distant locations.We aimed to identify the exosome miRNAs that may be involved in ...

ea0084op-12-63 | Oral Session 12: Nodules and Diagnostic | ETA2022

Real-world performance of a novel dual-component molecular assay in cytologically indeterminate thyroid nodules: a single institutional experience

Sponziello Marialuisa , Grani Giorgio , Verrienti Antonella , Pecce Valeria , Del Gatto Valeria , Bosco Daniela , Ascoli Valeria , Filetti Sebastiano , Durante Cosimo

Objective: We developed a novel dual-component molecular assay as an ancillary method to improve clinical decision-making in patients with cytologically indeterminate thyroid nodules. The assay includes next-generation sequencing (NGS) based detection of mutations in 23 thyroid cancer related genes and digital polymerase chain reaction (dPCR) evaluation of the expression levels of a microRNA strongly associated with thyroid cancer. The method was designed as a “rule-out&#...

ea0070aep995 | Thyroid | ECE2020

Performance of a dual-component molecular assay in cytologically indeterminate thyroid nodules

Sponziello Marialuisa , Verrienti Antonella , Pecce Valeria , Abballe Luana , Grani Giorgio , Ramundo Valeria , Fadda Guido , Brunelli Chiara , Durante Cosimo , Filetti Sebastiano

Background: Deciding whether patients with a cytologically indeterminate thyroid nodule should be referred for surgery or for active surveillance is an important challenge for clinicians. The aim of this study was to evaluate the performance of a novel dual-component molecular assay as an ancillary molecular method for resolving indeterminate thyroid nodule cytology.Methods: We selected 156 thyroid nodules from those that had undergone both FNA cytology ...

ea0081p458 | Thyroid | ECE2022

COVID-19 outbreak and de-escalation of thyroid cancer diagnosis and treatment

Grani Giorgio , Del Gatto Valeria , Ciotti Laura , Montesano Teresa , Biffoni Marco , Giacomelli Laura , Sponziello Marialuisa , Pecce Valeria , Verrienti Antonella , Durante Cosimo

Background: The COVID-19 outbreak in Italy forced the health system to cancel all non-urgent outpatient activities, to avoid further spreading of the disease inside the healthcare facilities. At our institution, for cancer patients the hospital allowed treatments and consultations: the medical team, however, identified patients whose procedures could be postponed. Even after May 2020, the capacity for non-urgent thyroid surgeries was reduced. These events enhanced our efforts ...

ea0081p720 | Thyroid | ECE2022

Diagnostic accuracy of ultrasonographic features in detecting thyroid cancer in the transition age: a meta-analysis from the TALENT group

Cozzolino Alessia , Filardi Tiziana , Simonelli Ilaria , Grani Giorgio , Virili Camilla , Stramazzo Ilaria , Giulia Santaguida Maria , Locantore Pietro , Maurici Massimo , Gianfrilli Daniele , M Isidori Andrea , Durante Cosimo , Pozza Carlotta

Context: Significant uncertainty exists about the diagnostic accuracy of ultrasonographic (US) features used to predict the risk of thyroid cancer in the pediatric population. Moreover, there are not specific indications for thyroid nodule evaluation in patients during the transition age.Objective: The meta-analysis aimed to address the following question: which thyroid nodule US features have the highest accuracy in predicting malignancy in the transiti...

ea0084op-07-34 | Oral Session 7: Thyroid Cancer Basic | ETA2022

CDK4 phosphorylation status and rational use of CDK4/6 inhibitors in advanced thyroid cancers

Pita Jaime M , Coulonval Katia , Costante Giuseppe , Myriam Decaussin , Emmanuelle Leteurtre , Cavaco Branca , A Copland John , Durante Cosimo , Maenhaut Carine , Dumont Jacques , Roger Pierre , Raspe Eric

Introduction and objectives: The cyclin-dependent kinases CDK4 and CDK6 are key regulators of the cell cycle entry, by phosphorylating the onco-suppressor retinoblastoma protein (pRb). CDK4/6 inhibitors (CDK4i) are already established as standard first-line treatment against advanced Estrogen Receptor-positive breast tumors, and have emerged as novel drugs to treat various pRb-proficient chemotherapy-resistant cancers. Presence of activating T172-phosphorylation of CDK4 in bre...

ea0070aep860 | Thyroid | ECE2020

The natural history of benign thyroid nodules: 10 years follow-up

Ramundo Valeria , Grani Giorgio , Bruno Rocco , Costante Giuseppe , Meringolo Domenico , Puxeddu Efisio , Torlontano Massimo , Tumino Salvatore , Lucisano Giuseppe , Nicolucci Antonio , Filetti Sebastiano , Durante Cosimo

Background and Aim: The incidental detection of asymptomatic thyroid nodules (TNs) has increased over time. Over 90% of newly diagnosed TNs are either cytologically or sonographically benign. This study aimed to determine the frequency and magnitude of changes in TNs size over time.Methods: We performed a prospective, multicenter, observational study involving consecutive euthyroid patients with 1–4 asymptomatic, sonographically or cytological...

ea0073oc5.5 | Oral Communications 5: Thyroid | ECE2021

Role of miR-139–5p in radioiodine-refractory thyroid cancers

Pecce Valeria , Abballe Luana , Sponziello Marialuisa , Brunelli Chiara , Verrienti Antonella , Annunziata Salvatore , Grani Giorgio , Fadda Guido , Salvatori Massimo , Russo Diego , Durante Cosimo

Radioiodide 131I (RAI) is the therapy of choice for radioiodine-avid differentiated thyroid cancer (DTC). However, 5–15% of DTC patients become RAI refractory (RAIR), and the 10-year survival rate for metastatic disease decreases to 10%. The failure of RAI response is mainly due to the loss of thyroid differentiation, that leads to the loss of expression/function of components of iodide metabolism, first of all the Na/I symporter (NIS). The finding that the MAPK pathway i...