Searchable abstracts of presentations at key conferences in endocrinology

ea0081p96 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Increased haematocrit mediates lowering of blood glucose in mice exposed to hypoxia or treated with erythropoetin

Scherer Thomas , Metz Matthaeus , Beghini Marianna , Durr Sabine , Corina Luca Andreea , Kaplanian Mairam , Abu Eid Sameer , Kautzky-Willer Alexandra , Furnsinn Clemens

Introduction: Mountain dwellers show a lower prevalence of diabetes, which among countless other factors could be due to lower partial oxygen pressure at high altitude. The present study was to investigate in an experimental setting, if and how reduced oxygen availability affects blood glucose.Methods: Male obese mice on high fat diet were continuously maintained under a normobaric hypoxic atmosphere (10% oxygen) for three months. To exclude indirect eff...

ea0081ep362 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Imidazole-osmium reduces elution of lipids from cryofixed rat hepatic tissue for ultrastructural analysis

Durr Sabine , Reipert Siegfried , Krššak Martin , Philippe Cecile , Ehret Viktoria , Luft Matthias , Schintlmeister Arno , Scherer Thomas , Furnsinn Clemens

Introduction: Transmission electron microscopy (TEM) is the main tool for studying the ultrastructural properties of metabolically diseased tissue. For impactful micrographs as well as for interpretation of TEM specimens, the integrity of cellular components is crucial. Elution of substances is a major problem in TEM preparation with no universal solution found so far. Investigating fatty liver disease in a rat model, an important factor is the depiction of undamaged lipid dro...