Endocrine Abstracts

1=School of Sciences, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK 2= Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria3= IRiD Biosciences, Wilding Road, Ball Green, Stoke-on-Trent, ST6 8BQAim: Vernonia amygdalina extract is used traditionally for type 2 diabetes treatment in developing countries. Studies have reported antidiabetic properties of aqueous...

Aim: Gallic acid is one of the major bioactive phenolic compounds that have been isolated from several plant extracts with reported antidiabetic actions. However, insulinotropic effects and mechanisms underlying antidiabetic actions of garlic acid is poorly understood. This study investigated insulin-releasing and glucose-lowering effects of gallic acid.Methods: Glucose-stimulated insulin secretion in BRIN-BD11 cells were examined in the absence or prese...

Aim: Ocimum basilicum extract is used traditionally for type 2 diabetes treatment in developing countries. Studies have reported antidiabetic properties of aqueous and methanolic extracts of O. basilicum but mechanisms underlying these actions are not fully understood.Methods: Insulin-releasing effect of O. basilicum extract (0.1 – 1000µg/ml) were investigated using BRIN-BD11 cells. Effects of the extract (100µg/...

Aim: Our previous studies indicated the potential antidiabetic actions of the amphibian host-defence peptide, tigerinin-1. Tigerinin-1R was conjugated with gastric inhibitory polypeptide (GIP) as part of strategies for further development of its therapeutic effects. This study characterises the insulin-releasing and cytotoxic properties of the resulting hybrid peptide.Method: Pure G-TGN was commercially synthesized G-TGN. Insulin releasing effects were a...

Aim: The use of commonly consumed vegetables, such as Pterocarpus mildbraedii, in the treatment of metabolic conditions is consistent with the food-as-medicine strategy. To characterise its pharmacological actions, this study assessed insulin-releasing and glucose lowering effects of effects of P. mildbraedii in models of type 2 diabetes.Methods: Clonal pancreatic cells, BRIN-BD11, were treated P. mildbraedii extracts...

Aim: Previous reports indicate therapeutic potentials of tigerinin-1R, isolated from the skin secretions of Hoplobatrachus rugulosus, as an antidiabetic agent. Despite the report of its insulin-releasing effects, the peptide’s mechanism of actions is poorly understood. This study investigates the impact of conjugation of tigerinin-1R with (dAla2)-GIP on the cellular uptake and anti-diabetic actions of the peptide.Method: Amphipathicity, net...