Searchable abstracts of presentations at key conferences in endocrinology

ea0070aep252 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Immunomodulatory and anti-diabetic actions of a structurally-modified analogue of esculentin-2CHa isolated from the skin secretion of the frog, lithobates chiricahuensis (ranidae)

Ojo Opeolu , Ofosu Wendy , Abiodun Falobi Ayodele , Benjamin Falana Ayokunle , Sen Monica , Smyth Lesley

Aim: Our previousstudies have shown evidence for insulin-releasing actions of some amphibian skin peptides; including esculentin-2CHa. However, there is a need to further improve the potency of some of this peptides and to characterize their other beneficial effects. This study investigates the effect of a Leu31→Lys substitution on the insulin-releasing and immunomodulatory effects of esculentin-2CHa.Methods: BRIN-BD11 cells were incubat...

ea0070aep364 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

In vitro and in vivo insulinotropic actions of magainin-AM1 on pancreatic beta cells and high fat fed mice

Abiodun Falobi Ayodele , Benjamin Falana Ayokunle , Abdel-Wahab Yasser HA , Flatt Peter R , Ojo Opeolu

Aim: Insulin-releasing effects and potential anti-diabetic actions of peptides isolated from the skin secretion of the African clawed frog, Xenopus amieti has been reported. However, peptides such as magainin-AM1 has not been previously studied for its ability to stimulate insulin secretion or promote glucose uptake. This study investigated insulin releasing effects as well as possible underlying mechanisms.Methods: Acute and chronic actions of ...

ea0099p279 | Diabetes, Obesity, Metabolism and Nutrition | ECE2024

Extracts of Vernonia amygdalina stimulated non-toxic insulin secretion from clonal pancreatic cells and improved glucose tolerance in mice with diet-induced obesity-diabetes

Falana Ayokunle , Heer Simren , Falobi Ayodele , Adie Moji , Edeani Joy , Ogundepo O , Ojo Opeolu

1=School of Sciences, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK 2= Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria3= IRiD Biosciences, Wilding Road, Ball Green, Stoke-on-Trent, ST6 8BQAim: Vernonia amygdalina extract is used traditionally for type 2 diabetes treatment in developing countries. Studies have reported antidiabetic properties of aqueous...

ea0104p88 | Diabetes & Metabolism | SFEIES24

Insulin-releasing and glucose-lowering effects of gallic acid in cellular and high fat-fed mice models of type 2 diabetes

Ojo Opeolu , Falana Ayokunle , Falobi Ayodele , Heer Simren , Adie Mojisola , Edeani Joy , Ojo Constance

Aim: Gallic acid is one of the major bioactive phenolic compounds that have been isolated from several plant extracts with reported antidiabetic actions. However, insulinotropic effects and mechanisms underlying antidiabetic actions of garlic acid is poorly understood. This study investigated insulin-releasing and glucose-lowering effects of gallic acid.Methods: Glucose-stimulated insulin secretion in BRIN-BD11 cells were examined in the absence or prese...

ea0104p92 | Diabetes & Metabolism | SFEIES24

Investigation of the mechanism underlying the antidiabetic actions of extracts of Ocimim basilicum

Falana Ayokunle , Falobi Ayodele , Kaur Simren , Edeani Joy , Adie Moji , Ojo Opeolu

Aim: Ocimum basilicum extract is used traditionally for type 2 diabetes treatment in developing countries. Studies have reported antidiabetic properties of aqueous and methanolic extracts of O. basilicum but mechanisms underlying these actions are not fully understood.Methods: Insulin-releasing effect of O. basilicum extract (0.1 – 1000µg/ml) were investigated using BRIN-BD11 cells. Effects of the extract (100µg/...

ea0090oc10.2 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 2 | ECE2023

Characterization of the insulinotropic and glucose lowering actions of hybridized amphibian host defence peptide, tigerinin-1R

Abiodun Falobi Ayodele , Edeani Joy Nneka , Ofosu Wendy Amy , Smyth Lesley , Corcoran Olivia , Dunmore Simon , Ojo Opeolu

Aim: Our previous studies indicated the potential antidiabetic actions of the amphibian host-defence peptide, tigerinin-1. Tigerinin-1R was conjugated with gastric inhibitory polypeptide (GIP) as part of strategies for further development of its therapeutic effects. This study characterises the insulin-releasing and cytotoxic properties of the resulting hybrid peptide.Method: Pure G-TGN was commercially synthesized G-TGN. Insulin releasing effects were a...

ea0090p74 | Diabetes, Obesity, Metabolism and Nutrition | ECE2023

Assessment of insulin-releasing and glucose-lowering actions of aqueous extracts of Pterocarpus mildbraedii cellular and diet-induced mice models of type 2 diabetes

Ojo Opeolu , Benjamin Falana Ayokunle , Abiodun Falobi Ayodele , Kaur Heer Simren , Adie Mojisola , Nneka Edeani Joy , Anyanwu Gabriel , Ejike Uju

Aim: The use of commonly consumed vegetables, such as Pterocarpus mildbraedii, in the treatment of metabolic conditions is consistent with the food-as-medicine strategy. To characterise its pharmacological actions, this study assessed insulin-releasing and glucose lowering effects of effects of P. mildbraedii in models of type 2 diabetes.Methods: Clonal pancreatic cells, BRIN-BD11, were treated P. mildbraedii extracts...

ea0099p482 | Diabetes, Obesity, Metabolism and Nutrition | ECE2024

The conjugation of tigerinin-1r with modified gastric inhibitory polypeptide enhanced its cellular uptake and metabolic actions

Ojo Opeolu , Abiodun Falobi Ayodele , Nneka Edeani Joy , Amy Ofosu Wendy , Smyth Lesley , Corcoran Olivia , Dunmore Simon

Aim: Previous reports indicate therapeutic potentials of tigerinin-1R, isolated from the skin secretions of Hoplobatrachus rugulosus, as an antidiabetic agent. Despite the report of its insulin-releasing effects, the peptide’s mechanism of actions is poorly understood. This study investigates the impact of conjugation of tigerinin-1R with (dAla2)-GIP on the cellular uptake and anti-diabetic actions of the peptide.Method: Amphipathicity, net...