Searchable abstracts of presentations at key conferences in endocrinology

ea0026p37 | Adrenal cortex | ECE2011

Thoracic and gastroenteropancreatic (GEP) neuroendocrine tumors (NETs) and the ectopic ACTH syndrome (EAS)

Kamp K , Alwani A , Feelders R A , de Herder W W

Introduction: The ectopic ACTH syndrome (EAS) is associated with a variety of malignancies, mostly of (neuro-)endocrine origin. Several series report on the relative contribution of EAS in the spectrum of Cushing’s syndrome. However, information on the incidence/prevalence of EAS in the setting of patients diagnosed with thoracic or gastroenteropancreatic (GEP) neuroendocrine tumours (NETs) is virtually absent.Study design: In our tertiary academic ...

ea0029p68 | Adrenal cortex | ICEECE2012

MGMT expression in human adrenocortical carcinoma cell lines and tissues and effects of temozolomide on tumor cell growth.

Koetsveld P. van , Feelders R. , Dogan F. , Dungen R. van den , Herder W. de , Hofland L.

Background: In case of inoperable disease or tumor recurrence, there are only a few medical treatment options for patients with adrenocortical carcinoma (ACC). The oral alkylating drug Temozolomide (TMZ) shows efficacy in a subset of patients with melanoma, glioblastoma or pancreatic neurendocrine tumours. Response to TMZ seems determined by the tumoral expression level of the DNA repair enzyme O(6)-Methylguanine-DNA methyltransferase (MGMT).Aims: To eva...

ea0026p33 | Adrenal cortex | ECE2011

Response of human adrenocortical carcinoma cell lines to cytostatic drugs and mitotane and the role of the multidrug resistance pump MDR1

van Koetsveld P M , Dogan F , Misier M B , Feelders R A , de Herder W W , Hofland L J

Introduction: In case of inoperable disease or tumor recurrence, therapy with mitotane (MIT), sometimes in combination with doxorubicin (DOX), etoposide (ETO), cisplatin (CIS) or streptozocin (STREP), is frequently applied in patients with adrenocortical carcinoma (ACC). The multidrug resistance pump MDR1 is responsible for resistance of tumor cells to cytostatic drugs. The activity of MDR1 is inhibited by the calcium-channel blocker verapamil. There are equivocal data in the ...

ea0029p10 | Adrenal cortex | ICEECE2012

The mTOR-pathway in normal and tumoral human adrenocortical tissues

De Martino M. , van Koetsveld P. , Feelders R. , Sprij-Mooij D. , Waaijers M. , Lamberts S. , de Herder W. , Colao A. , de Krijger R. , Pivonello R. , Hofland L.

Background: Novel treatment options are required for patients with adrenocortical carcinomas (ACCs). mTOR-inhibitors are anti-neoplastic drugs that target the mTOR-pathway.Aim: To describe the expression of the mTOR-pathway in normal and pathological adrenocortical tissues.Methods: We evaluated mRNA expression levels of mTOR, S6K and 4EBP1 in 10 normal adrenals (NA), 11 adrenal hyperplasias (AH), 19 adrenal adenomas (ACA) and 26 AC...

ea0029p11 | Adrenal cortex | ICEECE2012

Measurement of cortisol in scalp hair can be a new tool in the diagnosis and follow-up of patients with Addison’s Disease and (cyclic) Cushing’s Syndrome

Manenschijn L. , Koper J. , van den Akker E. , de Heide L. , de Jong F. , Feelders R. , Lamberts S. , van Rossum E.

Introduction: The diagnosis of Cushing’s Syndrome (CS) and in particular cyclic CS can be complicated. Standard screening tests for CS are the measurement of cortisol in 24-hours urine collections and in midnight saliva. In case of cyclic CS, results of these tests can be normal in between periods of hypercortisolism. The development of a method to measure cortisol in scalp hair provides the opportunity to investigate historical cortisol levels of months to years ago, wit...

ea0029p1073 | Neuroendocrinology | ICEECE2012

Normalization of cortisol levels in Cushing's disease after medical pretreatment before surgery: effects on somatostatin receptor subtype expression and in vitro response to somatostatin analogs.

Pas R. van der , Feelders R. , Bruin C. de , Pereira A. , Sprij-Mooij D. , Waaijers M. , Schulz S. , Lamberts S. , Hofland L.

Introduction: Corticotroph pituitary adenomas that cause Cushing’s disease (CD) predominantly express the dopamine 2 receptor (D2) and somatostatin receptor subtype (sst) 5. The expression of sst2 is relatively low because of downregulating effects of high endogenous cortisol levels. This may explain why the sst2-preferring somatostatin analog octreotide is not effective in CD. To assess whether normalization of urinary free cortisol (UFC) excretion modulates the sst expr...

ea0026oc5.4 | Hormone metabolism and action | ECE2011

Adrenal melanocortin 2 receptor associated protein (MRAP) expression is regulated by ACTH and angiotensin II, whereas MRAP2 levels are associated with ACTH responsiveness

Hofland J , Delhanty P J , Steenbergen J , Hofland L J , van Koetsveld P M , van Nederveen F H , de Herder W W , Feelders R A , de Jong F H

Introduction: ACTH stimulates adrenocortical steroid production through the melanocortin 2 receptor (MC2R). MC2R trafficking and signaling depend on the melanocortin 2 receptor accessory protein (MRAP). The MRAP-homolog MRAP2 also transports the MC2R but prevents effective activation. We investigated expression of these components of the MC2R complex in human adrenal tissues in order to uncover novel regulatory mechanisms and their contribution to ACTH responsiveness.<p cl...

ea0026p188 | Neuroendocrinology | ECE2011

Glucocorticoid receptor gene polymorphisms are associated with disease course during pregnancy and the post-partum period in rheumatoid arthritis

Quax R A M , de Man Y A , Koper J W , Willemsen S P , Lamberts S W J , Hazes J M W , Dolhain R J E M , Feelders R A

Background: The mechanism underlying the spontaneous improvement of rheumatoid arthritis (RA) during pregnancy and the subsequent post-partum flare is incompletely understood. The glucocorticoid receptor (GR) polymorphisms Bcl1 and 9β are associated with an increased and decreased glucocorticoid sensitivity respectively. We examined the relation between carriership of these functional GR polymorphisms and disease course during pregnancy and post-partum in RA...

ea0026p351 | Steroid metabolism | ECE2011

Evaluation of a method to measure long term cortisol levels in health and disease

Manenschijn L , Koper J W , van den Akker E L T , Feelders R A , Lamberts S W J , van Rossum E F C

Introduction: Elevated levels of cortisol are known to induce a wide range of pathology, e.g. abdominal obesity, type 2 diabetes and cardiovascular disease. Measuring serum and saliva cortisol is limited to one time point. Measurement of cortisol in scalp hair is a recently developed method to measure long term cortisol levels and might reflect cortisol exposure in patients with hyper- or hypo-cortisolism and in healthy persons. Our aims were to explore this measurement of cor...

ea0049gp116 | Endocrine Nursing | ECE2017

Recovery of the hypothalamus-pituitary-adrenal axis after transsphenoidal surgery for pituitary adenomas

van Eck J P , Neggers S J C M M , Janssen J A M J L , de Rijke Y B , Dallenga A H G , Nagtegaal A P , van der Lely A J , Feelders R A

Purpose: Hypopituitarism is frequently associated with lifelong replacement therapy. However, it has been suggested that recovery from hypopituitarism after transsphenoidal surgery (TSS) for pituitary adenomas may occur during follow up. The aim of this study was to assess the frequency of hypothalamus-pituitary-adrenal (HPA) axis recovery after TSS in patients with non-functioning pituitary adenomas and acromegaly.Methods: Over the 3-year observational ...