Searchable abstracts of presentations at key conferences in endocrinology

ea0041ep853 | Pituitary - Basic | ECE2016

Human non-functioning pituitary tumors invasiveness: inhibitory effects of dopamine receptor type 2 (DRD2) agonist and cofilin involvement

Peverelli Erika , Giardino Elena , Treppiedi Donatella , Locatelli Marco , Vaira Valentina , Ferrero Stefano , Spada Anna , Mantovani Giovanna

Non-functioning pituitary tumors (NFPTs), although benign in nature, frequently present local invasiveness that strongly reduces neurosurgery success. Medical therapy is still under debate, although dopamine (DA) receptor 2 (DRD2) agonists may induce tumor shrinkage in some patients. Aims of this study were: 1) to evaluate the effect of DR2D agonist BIM53097 on migration and invasion of NFPT cells, 2) to investigate the molecular mechanisms regulating the motility of these cel...

ea0022p720 | Signal transduction | ECE2010

Role of filamin-A in the regulation of dopamine D2 receptor localization and signaling in a lactotroph cell model

Mantovani Giovanna , Peverelli Erika , Ferrero Stefano , Vitali Eleonora , Beck-Peccoz Paolo , Spada Anna , Lania Andrea

Dopamine agonists (DA) inhibit prolactin (PRL) secretion and expression on the pituitary lactotrophs by binding dopamine D2 receptor. Prolactinomas are the most common secreting pituitary adenomas, and DA are the first choice for their treatment because they reduce PRL levels and tumor size. However, a subset of patients is resistant to DA. The mechanisms involved in DA resistance are not fully understood, although decreased expression of D2 receptor or altered signal transduc...

ea0020p585 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Genetic alterations of phosphodiesterase 11A (PDE11A) in acromegalic patients

Peverelli Erika , Ermetici Federica , Filopanti Marcello , Lania Andrea , Ferrero Stefano , Beck-Peccoz Paolo , Spada Anna

Background: Aberrant cAMP signalling is involved in the pathogenesis of somatotropinomas. Recently, variants of phosphodiesterase type 11A (PDE11A) gene have been described in patients with adrenocortical tumors. Aim of the study was to investigate the presence of these variants in patients with somatotropinomas.Subjects and methods: Germ-line DNA of 78 acromegalic patients was screened for known genetic variants of PDE11A by direct sequencing or restric...

ea0049ep1432 | Thyroid (non-cancer) | ECE2017

Expression analyses of HABP2 in neoplastic and normal thyroid tissues: could this gene play a role in the pathogenesis of familial non medullary thyroid cancer?

Muzza Marina , Colombo Carla , Proverbio Maria Carla , Ercoli Giulia , Perrino Michela , Cirello Valentina , Vicentini Leonardo , Ferrero Stefano , Fugazzola Laura

Recently, the G534E variant of the HABP2 gene was reported as the underlying genetic defect in large kindred with non-syndromic familial non medullary thyroid cancer (FNMTC), but these data were not confirmed in additional cohorts. Consistently, we found in our wide series of FNMTC that the HABP2G534E variant is frequent, but does not segregate with the disease. Nevertheless, a possible role for this gene in the pathogenesis of FNMTC cannot definitely ruled out also because co...

ea0041gp111 | Endocrine Tumours | ECE2016

Implication of Filamin A in pulmonary neuroendocrine tumors aggressiveness and progression

Vitali Eleonora , Ferrero Stefano , Peverelli Erika , Veronesi Giulia , Alloisio Marco , Santambrogio Luigi , Spada Anna , Mantovani Giovanna , Lania Andrea

Pulmonary neuroendocrine tumors (PNTs) comprise a spectrum of neoplasms, ranging from low grade carcinoids to the highly malignant small cell lung cancers. Several studies identified cytoskeleton protein Filamin A (FLNA) as determinant in cancer progression and metastasis. To date, the role of FLNA in PNTs aggressiveness and progression is still unknown.To address this question, we decided: i) to evaluate FLNA expression in PNTs ranging from typical carc...

ea0014p109 | (1) | ECE2007

Histopathological and molecular studies in patients with goiter and hypercalcitoninemia: reactive or neoplastic C-cell hyperplasia?

Verga Uberta , Ferrero Stefano , Vicentini Leonardo , Brambilla Tatiana , Cirello Valentina , Muzza Marina , Beck-Peccoz Paolo , Fugazzola Laura

The cut-off values able to differentiate between reactive or neoplastic C cell hyperplasia (CCH) or to predict sporadic medullary thyroid cancer (MTC) are still debated both for basal and stimulated calcitonin (bCT and sCT). Aim of the present study was to define the prevalence and the histological patterns of CCH in 15 patients with multinodular goiter (MNG), bCT>10 pg/ml and sCT levels >50 pg/ml. These data were compared with those from 16 patients with MNG and bCT l...

ea0081p300 | Calcium and Bone | ECE2022

BCYRN/BC200: identification of a novel circulating biomarker of parathyroid carcinoma

Morotti Annamaria , Cetani Filomena , Passoni Giulia , Borsari Simona , Guarnieri Vito , Verdelli Chiara , Stefania Tavanti Giulia , Ferrero Stefano , Corbetta Sabrina , Vaira Valentina

Parathyroid carcinomas (PCas) are rare endocrine malignant neoplasia characterized by an abnormal PTH secretion and, consequently, severe and uncontrolled hypercalcemia. Clinically, the preoperative diagnosis can be misleading, because PCas share some molecular and clinical similarities with benign lesions and are often indolent. The histological diagnosis of carcinoma is achieved based on the presence of mitotic nuclear figures, capsular invasion, parenchyma infiltration, vas...

ea0041oc14.1 | Thyroid Cancer | ECE2016

Tumor and normal thyroid stem-like cells: from tissues to zebrafish

Cirello Valentina , Vaira Valentina , Gaudenzi Germano , Stellaria Grassi Elisa , Vitale Giovanni , Ricca Dario , Bosari Silvano , Vicentini Leonardo , Persani Luca , Ferrero Stefano , Fugazzola Laura

Introduction: Cells with stem-like properties have been reported in benign and malignant thyroid diseases, and can be propagated by culturing them as non-adherent spheres.Design: Aim of the present study was to widely characterize the stem-like cells in tumor and normal thyroid tissues and in the corresponding in vitro-cultured thyrospheres, and to investigate in vivo the proangiogenic potential of thyrospheres in a zebrafish model....

ea0041gp115 | Endocrine Tumours | ECE2016

Loss of cells expressing the T-box transcription factor TBX1 might be associated with a quiescent phenotype in parathyroid tumours

Verdelli Chiara , Avagliano Laura , Ferrero Stefano , Guarnieri Vito , Cetani Filomena , Vicentini Leonardo , Beretta Edoardo , Scillitani Alfredo , Bulfamante Gaetano , Vaira Valentina , Corbetta Sabrina

Embryonic transcription factors have been involved in tumourigenesis. The transcription factor TBX1 regulates the embryonic parathyroid cells fate. Indeed, it has never been investigated in human adult parathyroids. Here, expression, function and regulation of the TBX1 gene were analyzed in adult normal and tumour parathyroid tissues. Immunohistochemistry identified 30–70% (mean±S.E.M, 52.0±7.3%) of cells expressing TBX1 at nuclear levels...

ea0041gp118 | Endocrine Tumours | ECE2016

Expression and regulation of the early embryonic stem cell genes in parathyroid tumours

Verdelli Chiara , Forno Irene , Ferrero Stefano , Guarnieri Vito , Scillitani Alfredo , Cetani Filomena , Vicentini Leonardo , Balza Gianni , Beretta Edoardo , Creo Pasquale , Vaira Valentina , Corbetta Sabrina

An embryonic gene signature has been suggested in parathyroid tumours. We investigated the expression of early embryonic stem cell (ESC) genes in parathyroid tumours. POU5F1/OCT4, SOX2 and NANOG transcripts were detected in almost all parathyroid adenomas (PAds; n=22) and atypical PAds (n=3), besides the variable expression of ESC genes KLF4, EGR1, and REX1/ZFP42. OCT4, SOX2 and NANOG proteins expression w...