Searchable abstracts of presentations at key conferences in endocrinology

ea0042p6 | (1) | Androgens2016

The bi-directional interaction of AR and IL6 signalling in the response to enzalutamide in prostate cancer cells

Handle Florian , Erb Holger HH , Luef Birgit , Hoefer Julia , Dietrich Dimo , Parson Walther , Kristiansen Glen , Santer Frederic R , Culig Zoran

Chronic inflammation and high expression of pro-inflammatory cytokines, such as Interleukin-6 (IL6), are well-known risk factors for prostate cancer (PCa). IL6 is known to activate the androgen receptor (AR) and has been implicated in development of castration resistance. Therefore, we wanted to investigate the interaction of AR and IL6 signalling and the effect on anti-androgen treatment. We could confirm IL6 mediated activation of the AR on 3 AR target genes in presence of R...

ea0042p30 | (1) | Androgens2016

Critical Role of Androgen Receptor Level in Prostate Cancer Cell Resistance to New Generation Antiandrogen Enzalutamide

Hoefer Julia , Akbor Mohammady , Handle Florian , Ofer Philipp , Puhr Martin , Parson Walther , Culig Zoran , Klocker Helmut , Heidegger Isabel

Enzalutamide is an androgen receptor (AR) inhibitor approved for therapy of metastatic castration resistant prostate cancer. However, clinical application revealed that 30 to 40% of patients acquire resistance after a short period of treatment. Currently, the molecular mechanisms underlying such insensitivities are not completely understood, partly due to a lack of model systems. In the present study we established three different cellular models of enzalutamide resistance inc...

ea0042p19 | (1) | Androgens2016

The AR/NCOA1 signaling regulates prostate cancer migration by involvement of PRKD1

Luef Birgit , Handle Florian , Kharaishvili Gvantsa , Hager Martina , Rainer Johannes , Janetschek Gunter , Hruby Stephan , Englberger Christine , Bouchal Jan , Santer Frederic R , Culig Zoran

Due to the urgent need for new prostate cancer (PCa) therapies, the role of androgen receptor (AR) interacting proteins should be investigated. In this study we aimed to address whether the AR coactivator nuclear receptor coactivator 1 (NCOA1) is involved in PCa progression. Therefore, we tested the effect of long-term NCOA1 knockdown on processes relevant to metastasis formation. [3H]-thymidine incorporation assays revealed a reduced proliferation rate in ...

ea0071001 | Dimerization of the ligand-binding domain is crucial for proper functioning of the androgen receptor | BES2020

Dimerization of the ligand-binding domain is crucial for proper functioning of the androgen receptor

Sarah El Kharraz , Christine Helsen , Vanessa Dubois , Florian Handle , Claude Libert , Martin Van Royen , Adriaan Houtsmuller , Claes Ohlsson , Matti Poutanen , Nina Atanassova , Ekaterina Pavlova , Dirk Vanderschueren , Frank Claessens

The importance of the androgen receptor (AR) in the development and maintenance of the male phenotype is demonstrated by patients with androgen insensitivity syndrome (AIS). These patients carry germline mutations in the AR and display a mild to complete intersex phenotype. The AR belongs to the subfamily of steroid receptors, which need to form homodimers to execute their role as transcription factors. For estrogen receptors, dimerization via the DNA-binding domains as well a...