Endocrine Abstracts

Background: The standard, lifelong therapy of phenylketonuria (PKU) is natural protein-restricted diet completed with Phenylalanine (Phe)-free L-amino acid mixtures that provide the daily necessary micronutrients, such as iodine and selenium. Our main objective in this study was to assess the iodine and selenium status of patients with PKU, based on their adherence to the low-Phe diet, compared to a healthy control group. Secondly, we aimed to asses whether adherence to therap...

Objectives: Differentiation of orbital fibroblasts (OFs) into myofibroblastic phenotype contributes to tissue remodelling in thyroid eye disease (TED). Our study aimed to investigate the impact of TGF-β1 induced myofibroblast differentiation on hyaluronan turnover, with a particular focus on the expression of its key enzymes.Methods: OF cultures were established from tissue samples acquired from decompression surgeries of patients with TED (TED-OFs;...

Introduction: During the course of thyroid eye disease (TED) immune cells infiltrate the orbital connective tissue leading to overstimulation of orbital fibroblasts (OFs). In the persistent inflammatory environment, OFs differentiate into myofibroblasts or adipocytes, simultaneously creating two phenotypes with different morphology and function. Activated OFs secrete pro-inflammatory cytokines and growth factors and contribute to the prolonged inflammation, among other things ...

Objectives: Glucocorticoids (GCs) are the first line therapy in moderate to severe, active thyroid eye disease (TED). While their beneficial immunosuppressive effects are well known, direct connective tissue effects are less characterized. The accumulation of extracellular matrix component hyaluronan (HA) and increased proliferation rate of fibroblasts are hallmarks connective tissue changes in TED. We examined the effect of methylprednisolone (MP), prednisolone (P), hydrocort...

Introduction: In the pathogenesis of thyroid eye disease (TED) activated fibroblasts in the orbital connective tissue show increased proliferation, hyaluronan (HA) production, and adipogenic differentiation potential. As a result of these, the orbital tissue volume expands and the intraorbital pressure increases. Mechanical stimuli, including high intraorbital pressure, may lead to the activation of mechanosensitive receptors, such as Piezo1, whose presence in orbital fibrobla...