Searchable abstracts of presentations at key conferences in endocrinology

ea0063oc8.4 | Reproduction 1 | ECE2019

Conditional ablation of AMP-Activated Protein Kinase (AMPK) in GnRH neurons reveals specific roles in reproductive and metabolic homeostasis

Roa Juan , Franssen Delphine , Barroso Alexia , Ruiz-Pino Francisco , Vazquez Maria Jesus , Garcia-Galiano David , Castellano Juan Manuel , Gaytan Francisco , Dieguez Carlos , Pinilla Leonor , Lopez Miguel , Tena-Sempere Manuel

Reproduction is tightly coupled to body energy and metabolic status. GnRH neurons, the final output pathway for the brain control of reproduction, directly or indirectly receive and integrate multiple signals, including metabolic cues regulating reproductive function. Yet, the molecular underpinnings of such phenomenon remain largely unfolded. AMP-activated protein kinase (AMPK), a fundamental cellular sensor that becomes activated in conditions of energy deficit, has a key ro...

ea0056oc2.4 | Look who is controlling your gonads! | ECE2018

Novel role of central ceramide signaling in mediating obesity-induced precocious puberty

Heras Violeta , Castellano Juan Manuel , Fernandois Daniela , Velasco Inmaculada , Roa Juan , Vazquez Maria J. , Ruiz-Pino Francisco , Pineda Rafael , Torres Encarnacion , Avendano Maria Soledad , Gaytan Francisco , Pinilla Leonor , Lopez Miguel , Casals Nuria , Tena-Sempere Manuel

Childhood obesity has become a major health problem, which is coupled to different adverse outcomes and diseases. The escalating prevalence of child obesity parallels that of alterations in pubertal timing, also linked to higher disease burden later on life. Yet, the mechanisms underlying for this association remain unfolded. Ceramides, ubiquitous signaling molecules involved in numerous biological processes, have emerged as mediators of metabolic disorders and transmitters fo...

ea0056gp141 | Neuroendocrinology | ECE2018

Reproductive and metabolic consequences of AMP-Activated Protein Kinase (AMPK) ablation in GnRH neurons

Barroso Alexia , Franssen Delphine , Ruiz-Pino Francisco , Vazquez Maria Jesus , Garcia-Galiano David , Castellano Juan Manuel , Onieva Rocio , Rodriguez Ana Belen , Gaytan Francisco , Dieguez Carlos , Pinilla Leonor , Lopez Miguel , Roa Juan , Tena-Sempere Manuel

GnRH neurons are the final output of the brain controlling reproduction. These neurons receive information from multiple central and peripheral signals, ranging from gonadal steroids to metabolic cues and brain neuropeptides, to regulate their activity. AMP-activated protein kinase (AMPK) is an intracellular sensor, activated by energy deficiency, involved in the regulation of the cellular and whole-body energy homeostasis. Previous in vitro studies suggested the part...

ea0063gp62 | Reproductive Axis | ECE2019

Hypothalamic miR-30 regulates puberty onset via repression of the puberty-suppressing factor, Mkrn3

Castellano Juan M , Heras Violeta , Sangiao-Alvarellos Susana , Manfredi-Lozano Maria , Sanchez-Tapia Maria J , Ruiz-Pino Francisco , Roa Juan , Lara-Chica Maribel , Morrugares-Carmona Rosario , Abreu Ana P , Belsham Denise , Vazquez Maria J , Calzado Marco A , Pinilla Leonor , Gaytan Francisco , Latronico Ana C , Kaiser Ursula B , Tena-Sempere Manuel

Mkrn3, the maternally imprinted gene encoding the makorin RING-finger protein-3, has recently emerged as putative pubertal repressor, as evidenced by central precocity caused by MKRN3 mutations in humans; yet, the molecular underpinnings of this key regulatory action remain largely unexplored. We report herein that the microRNA, miR-30, with three binding sites in a highly conserved region of its 3’-untranslated region (UTR), operates as repressor of Mkrn3 to control pube...