Searchable abstracts of presentations at key conferences in endocrinology

ea0049ep196 | Neuroendocrinology | ECE2017

Identification of molecular targets of Sunitinib in pancreatic neuroendocrine tumours

Bresciani Giulia , Gagliano Teresa , Hofland Leo J , Gentilin Erica , Falletta Simona , Riva Eleonora , Chiara Zatelli Maria

: Pancreatic neuroendocrine tumours (pNETs) are rare neoplasms arising from the endocrine pancreas. The fist line treatment is surgery that is often not curative in the presence of metastatic disease. Therefore, there is an increasing need for medical therapy. Sunitinib is a multi-target receptor tyrosine-kinase (RTK) inhibitor, described as having as main target VEGF receptor, with antitumor and antiangiogenic effects, approved for pNETs medical treatment. This study is aimed...

ea0049ep197 | Neuroendocrinology | ECE2017

Study of different in vitro systems for the evalutation of Sunitinib effects in pancreatic neuroendocrine tumour cells

Bresciani Giulia , Gagliano Teresa , Hofland Leo J , Gentilin Erica , Falletta Simona , Riva Eleonora , Chiara Zatelli Maria

Finding new preclinical models to study the effects of anticancer drugs is one of the aims of biomedical research. Indeed, testing different in vitro systems can lead to a better understanding of the molecular pathways regulating tumor development and growth, and help to find new therapeutic approaches. This is crucial especially in the settings of pancreatic neuroendocrine tumours (pNET), where one of the main drugs approved for medical treatment is Sunitinib, a mult...

ea0035oc7.1 | IGF-1 and Thyroid Basic | ECE2014

GH induces chemoresistance in human endometrial cancer cell lines involving ERK 1/2 and PKCdelta

Zuolo Giulia , Minoia Mariella , Gentilin Erica , Gagliano Teresa , Tagliati Federico , Zatelli Maria Chiara , Uberti Ettore degli

Background: Surgery is the main therapeutic option for advanced endometrial cancer; however, when disease relapses, chemotherapy is the only option. Chemoresistance is a very common phenomenon in these tumors. We previously demonstrated that GH protects breast cancer cells towards the cytotoxic effects of doxorubicin, inducing chemoresistance. Recent evidences show that endometrial cancer cells secrete GH, stimulating their own growth in an autocrine fashion.<p class="abst...

ea0035p538 | Endocrine tumours and neoplasia | ECE2014

Cyclin D1 levels are involved in the resistance to m-TOR inhibitors in human bronchial carcinoids

Benfini Katiuscia , Gagliano Teresa , Gentilin Erica , Minoia Mariella , Daniela Mole , Uberti Ettore degli , Zatelli Maria Chiara

Background: Bronchial carcinoids (BC) are still orphan of medical therapy. We previously demonstrated that the typical BC human cell line NCI-H727 is sensitive to Everolimus, in terms of cell viability reduction, while the atypical human BC cell line NCI-H720 is not. However, the mechanisms underlying this phenomenon have not been fully clarified.Aim: The aim of our study is to investigate the mechanisms of resistance to mTOR inhibitors in BC cells.<...

ea0032p6 | Adrenal cortex | ECE2013

Activation of EGFR promotes ACC cell proliferation by inducing VEGF autocrine secretion

Gagliano Teresa , Mole Daniela , Gentilin Erica , Bellio Mariaenrica , degli Uberti Ettore , Zatelli Maria Chiara

Adrenocortical cancer (ACC) is a rare and aggressive malignancy. Currently the main therapeutic option is surgery, but due to difficult and delayed diagnosis and to the onset of metastases, medical therapy is often tried. ACC treatment is mainly represented by Mitotane alone or in association with chemotherapy, with variable results. Understanding the molecular mechanisms that regulate ACC proliferation could be useful to identify new therapeutic options. Aim of our study is t...

ea0032p539 | Endocrine tumours and neoplasia | ECE2013

Involvement of PKCβ and PKCδ isoforms in TSH signaling pathway in thyroid cancer cell lines

Mole Daniela , Gentilin Erica , Gagliano Teresa , Tagliati Federico , degli Uberti Ettore , Zatelli Maria Chiara

It is well established that most TSH effects on the thyroid gland, including stimulation of proliferation, thyroid hormone synthesis and expression of thyroid-specific genes, are transmitted mainly by the adenylate cyclise pathway. However, in human follicular cells and in rat FRTL-5 cells, TSH can also stimulate the β-isoforms of PLC that catalyzes the hydrolysis of phosphatidyl-inositol 4,5-phosphate, yielding the second messengers DAG and inositol 1,4,5-phosphate facil...

ea0041ep612 | Endocrine tumours and neoplasia | ECE2016

Human endometrial adenocarcinoma: growth hormone involvement in cancer chemoresistance

Gentilin Erica , Di Pasquale Carmelina , Benfini Katiuscia , Falletta Simona , Riva Eleonora , degli Uberti Ettore , Zatelli Maria Chiara

Introduction: It is known that growth hormone (GH) may influence neoplastic development of endometrial epithelium. GH is produced by normal and neoplastic endometrial cells and elevated levels have been observed in endometrial epithelium of patients with endometriosis and endometrial adenocarcinoma (EA). Moreover, endometrium cancer is one of the most occurring tumors in acromegalic patients. Furthermore, autocrine GH increases the oncogenicity of EA cells and is considered fu...

ea0041ep865 | Pituitary - Basic | ECE2016

mTOR pathway: its role in regulating GH secretion in a rat pituitary adenoma cell line

Di Pasquale Carmelina , Gentilin Erica , Bellio Mariaenrica , Benfini Katiuscia , Falletta Simona , Riva Eleonora , degli Uberti Ettore , Zatelli Maria Chiara

Acromegaly results from excess growth hormone (GH) secretion, due to a pituitary adenoma. Surgery is the first option recommended for treatment of GH secreting pituitary adenomas; medical therapy, mostly represented by somatostatin analogues (SSA), is most often used if surgery is not successful. Insulin-like Growth Factor-1 (IGF-1) physiologically reduces GH levels through an endocrine negative feedback loop. IGF-1 exerts its effects also through PI3K/Akt/mTO...

ea0035oc10.2 | Endocrine Tumours | ECE2014

The cytotoxic effect of sunitinib on human bronchial carcinoid cell lines and primary cultures is counteracted by EGF and IGF-1 but not by VEGF

Gagliano Teresa , Gennari Giulia , Tassinari Martina , Bellio Mariaenrica , Gentilin Erica , Benfini Katiuscia , Uberti Ettoredegli , Zatelli Maria Chiara

Background: Bronchial carcinoids (BC) are rare tumors originating from endocrine cells dispersed in the respiratory epithelium. The main BC treatment is surgery, which is not feasible for large, infiltrating and metastatic disease. In these settings, medical therapy is often tried. Therefore it is important to identify new therapeutic targets and new molecules capable of providing adequate medical treatment for patients with BC. Sunitinib, is an oral, small-molecule, multi-tar...

ea0035p11 | Adrenal cortex | ECE2014

VEGF autocrine secretion is enhanced by EGFR activation trough ERK1/2 phosphorylation in human adrenocortical carcinoma cell lines

Gagliano Teresa , Di Pasquale Carmelina , Gentilin Erica , Mole Daniela , Tassinari Martina , Bellio Mariaenrica , degli Uberti Ettore , Zatelli Maria Chiara

Adrenocortical cancer (ACC) is still orphan of medical treatment. Our preliminary data show that EGF induces ACC cell lines proliferation (+20 and +10% vs control in SW13 and NCI-H295 cell lines respectively). EGF receptor (EGFR) expression is higher and ubiquitous in SW13 cells, while it is weaker in NCI-H295 cells, where it is present only on the membrane. Aim of our study is to analyze EGFR downstream signalling in ACC cell lines.EGF induces VEGF synt...