Searchable abstracts of presentations at key conferences in endocrinology

ea0086p159 | Adrenal and Cardiovascular | SFEBES2022

An audit of the clinical utility of urine steroid profiling for endocrine disorder diagnosis in a routine clinical laboratory in 2021

Taylor David , Churchus Richard , Collins Heather , Ajaj Nicola , Ekundayo Susan , Alramadhi Ulfat , Rayner Oliver , Ghataore Lea

Measurement of steroid metabolites in urine by gas chromatography-mass spectrometry remains the gold standard for biochemical diagnosis of steroidogenic disorders. In the UK, three laboratories offer steroid profiling for routine clinical testing, with the Supraregional Assay Service at King’s College Hospital being the largest and experiencing increased demand year-on-year for testing. In this study, we used our profiling database to audit 2021 workload. By direct compar...

ea0028p317 | Steroids | SFEBES2012

Effects of mitotane on exogenous and endogenous steroid metabolism

Ghataore Lea , Chakraborti Indira , Aylwin Simon , Schulte Klaus-Martin , Taylor Norman

Objective: Mitotane (o,p’DDD) is an effective adrenolytic agent for treatment of advanced adrenocortical carcinoma (ACC). Suppression of cortisol biosynthesis, including inhibition of 11β-hydroxylation, has been described. Decreased cortisol bioavailability is indicated by increased dose requirement for hydrocortisone replacement during mitotane treatment. Urinary excretion of common cortisol metabolites (CM) has been reported to be normal but with increase of 6&#946...

ea0021p225 | Endocrine tumours and neoplasia | SFEBES2009

Mitotane treatment has profound effects on cortisol catabolism

Ghataore Lea , Abraha Hagosa , Chakraborti Indrani , Taylor Norman , Aylwin Simon , Schulte K-M

Mitotane (o,p’DDD) is an effective oral adrenolytic agent for treatment of advanced adrenocortical carcinoma (ACC). Inhibition of cortisol biosynthesis has been described. Cortisol metabolic clearance rate is unchanged, but decreased cortisol biovailability is indicated by increased dose requirement for hydrocortisone replacement during mitotane treatment. Urinary excretion of common cortisol metabolites (CM) has been reported to be normal but with increase of 6β-hyd...

ea0044p8 | Adrenal and Steroids | SFEBES2016

Full characterisation of adrenal steroidogenesis by liquid-chromatography–mass spectrometry (LC–MS/MS) in metyrapone and/or ketoconazole-treated pituitary/adrenal Cushing’s

Taylor David R , Leong Christine H M , Bhatt Aagna E , Ghataore Lea , Aylwin Simon , Whitelaw Ben , Vincent Royce P

Introduction: Pituitary and adrenal Cushing’s may be managed by pharmacological-inhibition of adrenal steroidogenesis, using metyrapone and/or ketoconazole. Assessment of biochemical control is challenging owing to cross-reactivity in immunoassays (e.g. cortisol and 11-deoxycortisol) leading to over/under-treatment. Off-target effects can also result, e.g. hyperandrogenism/mineralocorticoid hypertension (increased 11-deoxycorticosterone/DOC). LC-MS/MS analysis is free fro...

ea0035p948 | Steroid metabolism and action | ECE2014

Changes in serum steroid concentrations in relation to fat distribution and insulin resistance in women with polycystic ovarian syndrome

Ibanichuka Jacqueline , Vincent Royce P , Ghataore Lea , Taylor Norman F , Millward Ann , Pinkney Jonathan H , Stenhouse Elizabeth

Background: Polycystic ovarian syndrome (PCOS) affects 5–10% of women of reproductive age. Hyperandrogenism and hyperinsulinaemia have been attributed to the pathophysiology of PCOS. The aim of this study is to evaluate the effect of fat distribution and insulin resistance on steroid metabolism in PCOS.Methods: The study recruited 20 PCOS and 20 matched controls. Fat distribution was assessed by waist circumference, skin fold measurements, and body ...

ea0044p4 | Adrenal and Steroids | SFEBES2016

Generation of human urine-derived steroidogenic cells through lineage conversion: A new technology to study the adrenal gland

Ruiz-Babot Gerard , Hadjidemetriou Irene , Ajodha Sharon Jane , Ghataore Lea , Taylor David , Taylor Norman , Balyura Mariya , Bornstein Stefan R , Guasti Leonardo

Cellular reprogramming describes the process where a fully differentiated, specialized cell type is induced to transform into a different cell. Cell reprogramming techniques can become powerful tools for modelling diseases, drug testing and for personalized cellular therapy. The adrenal cortex is the primary site of steroid synthesis. Adrenal insufficiency, which can be life threatening, is caused by a number of adrenal disorders, and lifelong management of these patients with...

ea0038p398 | Steroids | SFEBES2015

Discrimination of adrenocortical carcinoma from other adrenal lesions: use of a new 13 steroid serum panel based on LC–MS/MS

Taylor David , Ghataore Lea , Vincent Royce , Sherwood Roy , Whitelaw Ben , Dworakowska Dorota , Schulte Klaus-Martin , Diaz-Cano Salvadore , Lewis Dylan , Aylwin Simon , Taylor Norman

Adrenocortical carcinoma (ACC) is a rare malignancy, but accounts for up to 11% of adrenal masses investigated in referral centres. Diagnosis remains a challenge. Up to two thirds are biochemically inactive, resulting from de facto enzyme deficiencies in the steroid hormone biosynthetic pathways, as shown by urine steroid profiling by gas chromatography-mass spectrometry. Increased metabolites of pathway intermediates in ACC discriminate it from benign adrenal lesions...