Searchable abstracts of presentations at key conferences in endocrinology

ea0014oc1.2 | Thyroid clinical | ECE2007

Expression gene profile may be useful for the diagnosis of thyroid malignancies

Piampiani Pamela , Romei Cristina , Cosci Barbara , Vivaldi Agnese , Ciampi Raffaele , Viola David , Bottici Valeria , Giannini Riccardo , Basolo Fulvio , Pinchera Aldo , Elisei Rossella

Although 20% of follicular neoplasms are papillary thyroid carcinoma (PTC), their cytological diagnosis is not diagnostic. A different profile of gene expression between malignant and benign thyroid tumors has been reported. Aim of this study was to identify a gene expression profile to be used in distinguishing malignant from benign thyroid neoplasms. By real-time RT-PCR we analyzed mRNA expression of 6 thyroid differentiation genes (TTF-1, PAX8, TPO, TSHr, NIS and Tg) and 5 ...

ea0014p366 | (1) | ECE2007

Prognostic significance of BRAF mutation in patients affected by papillary thyroid carcinoma with a follow up of 20 years

Viola David , Romei Cristina , Biagini Agnese , Agate Laura , Molinaro Eleonora , Ciampi Raffaele , Renzini Giulia , Giannini Riccardo , Ugolini Clara , Berti Piero , Pinchera Aldo , Elisei Rossella

BRAFV600E is the most common mutation in papillary thyroid carcinoma (PTC). Anatomo-patology and clinical features of PTC with BRAFV600E are well described in literature.Aim of this study was to examine the prognostic significance of BRAFV600E in patients with PTC and a follow-up of 15-20 years.Genomic DNA was purified from 67 paraffin-embedded tumoral tissue. A PCR-SSCP analysis of exon 15 of BRAF ...