Endocrine Abstracts

Introduction: Enteroendocrine cells (EECs) are hormone-secreting cells within the intestinal epithelium that play an important role in regulating food absorption, insulin secretion and appetite. To understand the molecular mechanism governing the function of EECs, it is important to investigate cell-type-specific gene expression. The aim of this study was to identify the transcriptome of human EEC populations from organoid culture.Methods: To label the f...

Objective: Motilin is a proximal small intestinal hormone with roles in gastrointestinal motility, gallbladder emptying and hunger initiation. The molecular mechanisms underlying motilin release in response to fats, bile and duodenal acidification are poorly understood, in part due a lack of suitable cellular and rodent models. We therefore generated a novel human intestinal organoid model with fluorescently labelled motilin-expressing M-cells, which we used to establish the k...