Endocrine Abstracts

Glargine is an analogue insulin which has a peakless action profile over approximately 24 hours. As a result it has been shown to reduce the frequency of hypoglycaemia thereby enabling achievement of tight glycaemic control. This is important during pregnancy in order to lower the risk of foetal malformations.Method: 17 women, mean age 28 years, with type 1 Diabetes Mellitus on a basal bolus regimen including Glargine became pregnant between January 2004...

Objective: The spontaneously hypertensive stroke prone rat (SHRSP) is a model of insulin resistance and dyslipidemia. Feeding a 60% fructose diet exaggerates these phenotypes. The Y chromosome may influence lipid levels in the spontaneously hypertensive rat (SHR). Therefore we aim to characterise the metabolic syndrome using in vivo and dyslipidemic phenotpyes in the SHRSP and examine the contribution of the Y chromosome.Methods: SHRSP and WKY Y consomic strains (SP...

Objective: To construct rat congenic strains to confirm and narrow down a region on rat chromosome 2 previously implicated in blood pressure regulation. To determine expression profiles and identify differentially expressed genes between congenic strain and respective parental strain using microarray technology.Design and Methods: Total RNA was prepared from whole kidney homogenates from 2x parental SHRSPGla and 2x SP.WKYGla2a congenic rats. Expression ...

Objective: The spontaneously hypertensive stroke prone rat (SHRSP) is a model of insulin resistance and dyslipidemia. Feeding a 60% fructose diet exaggerates these phenotypes. It has been suggested that the Y chromosome may influence lipid levels in the spontaneously hypertensive rat (SHR). Therefore we examined the effects of Y chromosome on lipid phenotypes in the SHRSP after fructose feeding.Design and Methods: The S.W. Y consomic strain was construc...

Insulin-like growth factor (IGF-1) has been postulated to function as a vasodilator via a nitric oxide (NO) pathway. IGF-1/growth hormone deficiency in humans is associated with endothelial dysfunction and increased cardiovascular mortality.We compared the effects of IGF-1 on vascular function in control Wistar-Kyoto (WKY) (n=6) and stroke-prone spontaneously hypertensive rats characterised by endothelial dysfunction (SHRSP) (n=7)....

Objective. The aims of our study were to utilise a combination of high fidelity phenotyping, microarray gene expression profiling and conserved synteny mapping between rodent and human genomes to identify genetic determinants underlying human hypertension.Methods. We used the stroke-prone spontaneously hypertensive rat (SHRSP) and a congenic strain (SP.WKYGla2c*) produced by introgressing a quantitative trait locus responsible for blood pressure regulation on rat chromosom...