Searchable abstracts of presentations at key conferences in endocrinology

ea0031oc1.5 | Young Endocrinologists prize session | SFEBES2013

Inhibition of 5α-reductase type 1 with dutasteride impairs insulin sensitivity

Upreti Rita , Hughes Katherine , Gray Calum , Minns Fiona , Marshall Ian , Stewart Laurence , Walker Brian , Andrew Ruth

5α-Reductase (5αR) inhibitors decrease prostatic dihydrotestosterone in benign prostatic hyperplasia (BPH) treatment; finasteride inhibits 5αR type 2, while dutasteride inhibits 5αR1 and 2. 5αRs, especially 5αR1, are also expressed in metabolic tissues regulating actions of androgens and other substrates, including glucocorticoids.Hypothesis: 5αR1 inhibition with dutasteride induces metabolic dyshomeostasis.<p class...

ea0094oc6.4 | Metabolism, Obesity and Diabetes | SFEBES2023

Human brown adipose tissue activity is regulated by the parasympathetic nervous system

Choong Kwok T'ng , Ramage Lynne E. , Gray Calum , Wakelin Sonia J. , JR van Beek Edwin , Kelman Alexandra , Semple Robert J. , Suchacki Karla J. , Stimson Roland H

Brown adipose tissue (BAT) is a therapeutic target for obesity and associated metabolic diseases, due to its role in non-shivering thermogenesis. BAT activation is mediated through sympathetic stimulation, but parasympathetic regulation of human BAT has not been demonstrated previously. We undertook RNA sequencing of human white and brown primary adipocytes to identify novel pathways regulating BAT. CHRM2 (encoding the muscarinic acetylcholine receptor 2) was the most differen...

ea0077oc3.1 | Metabolism, Obesity and Diabetes | SFEBES2021

The serotonin transporter SLC6A4 protects human brown adipose tissue from serotonin-mediated suppression of thermogenesis.

Choong Kwok T’ng , Suchacki Karla , Ramage Lynne , Kelman Alexandra , McNeill Ben , Rodney Stewart , Keegan Matthew , Gray Calum , Manning Jonathan , MacNaught Gillian , Fletcher Alison , Simpson Joanna , Carter Roderick , Morton Nicholas , Homer Natalie , van Beek Edwin , Wakelin Sonia , Stimson Roland

The recent discovery of brown adipose tissue (BAT) in adult humans, which generates heat to maintain body temperature in a cold environment, offers an exciting new strategy to treat obesity and metabolic disease, but our knowledge of human BAT activation is limited. To identify novel pathways regulating human BAT, we undertook RNA sequencing of human brown and white adipocytes. The gene SLC6A4 (encoding the serotonin transporter SERT) was one of the most highly differ...

ea0086oc5.1 | Metabolism, Obesity and Diabetes | SFEBES2022

Human brown adipose tissue demonstrates substantial choline uptake for incorporation into phosphatidylcholines

Suchacki Karla , Ramage Lynne , Gray Calum , Rodrguez Blanco Giovanny , Choong Kwok T'ng , Boyle Luke , MacNaught Gillian , Gregoriades Maria-lena , Wakelin Sonia , von Kriegsheim Alex , Finch Andrew , Patel Dilip , van Beek Edwin , Stimson Roland

Background: 18F-fluorodeoxyglucose (18FDG) PET is commonly used to quantify brown adipose tissue (BAT) mass/activity in humans but requires cold exposure. Rodent brown (BAds) but not white adipocytes (WAds) exhibit high choline content, thus we hypothesised that human BAT would demonstrate substantial 18F-fluorocholine (18FCH) uptake in vivo during warm and cold conditions.Methods: (1)...

ea0104s3.3 | Bone Update | SFEIES24

Identification of the genetic determinants and clinical implications of bone marrow adiposity using deep learning in the uk biobank

Xu Wei , Mesa-Eguiagaray Ines , Morris David , Wang Chengjia , Gray Calum , Sjostrom Samuel , Papanastasiou Giorgos , Badr Sammy , Paccou Julien , Li Xue , Timmers Paul , Timofeeva Maria , Semple Scott , MacGillivray Tom , Theodoratou Evropi , Cawthorn William

Bone marrow adipose tissue (BMAT) is a distinct, major adipose tissue subtype that is a normal feature of mammalian anatomy. However, BMAT’s pathophysiological functions and genetic determinants remain unknown. In humans, bone marrow adiposity is typically measured as the bone marrow fat fraction (BMFF) using magnetic resonance imaging (MRI). Herein, we used deep learning to measure the BMFF of the spine, femoral head, total hip, and femoral diaphysis from MRI of >45,...