Searchable abstracts of presentations at key conferences in endocrinology

ea0054p1 | (1) | NuclearReceptors2018

Single-molecule analysis of peroxisome proliferator-activated receptor γ2 and α reveal subtype specific differences in chromatin binding dynamics

Jensen Rikke AM , Paakinaho Ville , Presman Diego M , Swinstead Erin E , Schiltz R Louis , Ball David A , Karpova Tatiana S , Mandrup Susanne , Hager Gordon L

The peroxisome proliferator-activated receptors (PPARs) display a high degree of conservation in the DNA- and ligand-binding domains. Despite these sequence similarities, the PPARs show distinct functions, even when co-expressed. Many experimental approaches have been employed to determine the molecular mechanisms that underlie their subtype-specific characteristics. However, these approaches have largely relied on cell population based studies, such as ChIP-seq. Here we have ...

ea0044oc4.3 | Adrenal and Steroids | SFEBES2016

Genome wide ChIP-Seq analysis of Glucocorticoid Receptor and RNA Polymerase 2 binding in rat liver during physiological and non-physiological corticosterone replacement

Flynn Benjamin P. , Birnie Matthew T. , Kershaw Yvonne M. , Baek Songjoon , Kim Sohyoung , Stavreva Diana A. , Hager Gordon L. , Lightman Stafford L. , Conway-Campbell Becky L.

Ultradian glucocorticoid (GC) secretion is highly conserved, having been detected in all species studied. The GC corticosterone (CORT) is a ligand for the glucocorticoid receptor (GR), inducing GR recruitment to glucocorticoid responsive elements (GREs) to modulate transcription of GC-target genes. We have previously demonstrated that pulsatile GR recruitment to GREs upstream of the Period1 gene is associated with its pulsatile transcription in rat liver. Similarly th...

ea0038oc1.5 | Early Career Oral Communications | SFEBES2015

Interaction of the MR and GR in the nucleus and at DNA

Pooley John R , Presman Diego M , Davis Luke , Ganesan Sundar , Muratcioglu Serena , Keskin Ozlem , Schiltz Louis , Levi Valeria , Lightman Stafford L , Hager Gordon L

Glucocorticoid actions in the brain are mediated by glucocorticoid receptors (GR) and mineralocorticoid receptors (MR). MR and GR bind endogenously circulating glucocorticoids, share a hormone response element in DNA, and are co-expressed in neurons of the hippocampus and hypothalamus. This arrangement suggests MR and GR functionally cooperate in the regulation of gene expression though hitherto poorly described mechanisms. This possibility was previously exampled by the demon...

ea0038p385 | Steroids | SFEBES2015

Genome-wide binding analysis of glucocorticoid receptors in the rat hippocampus in response to corticosterone and stress

Flynn Benjamin P , Pooley John R , Kershaw Yvonne M , Baek Songjoon , Grontved Lars , Guertin Michael J , Hager Gordon L , Lightman Stafford L , Conway-Campbell Becky L

Glucocorticoids act via the glucocorticoid receptor (GR). Upon ligand binding, GR translocates to the nucleus and binds directly to glucocorticoid responsive elements (GREs) to regulate transcriptional output. Glucocorticoid secretion increases in response to stress to affect transcriptional output within specific areas of the brain including the hippocampus (HC). Therefore, here we assess changes in genome-wide GR chromatin binding profiles in the rat HC in response to a gluc...