Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep1165 | Clinical Cases–Pituitary/Adrenal | ECE2015

Histiocytosis, a rare cause of hypopituitarism. Langerhans cell histiocytosis and Erdheim–Chester disease, two case reports of pituitary deficiency

Hana Vaclav Kosak Mikulas , Hana Vaclav

Introduction: Langerhans cell histiocytosis (LCH) is a rare disease with incidence of 1–2 adults per milion/year. Pituitary function is affected in 30% of cases presenting almost always with diabetes insipidus. Other deficiencies such as GH, FSH+LH, TSH and ACTH follow. Erdheim–Chester disease is a rare Non-LCH described in several hundreds of cases in the world so far, with the pituitary function involvement in similar frequency as in LCH.Case...

ea0049ep208 | Steroid metabolism + action | ECE2017

Steroid metabolome changes in the 1 mg dexamethasone suppression test

Hana Vaclav , Kosak Mikulaš , Hana Vaclav , Hill Martin

Introduction: Dexamethasone induced negative feedback on CRH, ACTH and cortisol secretion is one of the key principles in the diagnostic workup of hypercortisolism. Cortisol secretion has been studied extensively, but changes of other steroids remained apart. Our aim was to establish steroid profile changes in 1 mg dexamethasone test.Methods: The Steroid profile consisting of 103 steroids and their metabolites from the serum of 9 male and 8 healthy femal...

ea0041ep33 | Adrenal cortex (to include Cushing's) | ECE2016

Androgen producing adenoma in a patient with non-diagnosed congenital adrenal hyperplasia

Kosak Mikulas , Hana Vaclav Michlasky David , Kubinyi Jozef , Hana Vaclav

Androgen secreting adrenal tumor is a rare cause of androgen excess in women. There are only few case-reports of androgen producing adenoma in a patient with congenital adrenal hyperplasia.Our patient, 61 year old woman was referred to our department for progressive hirsutism and androgenic alopecia. She did not mention neither excessive facial hair growth nor balding until age of 59 when rapidly progressive balding and overt hirsutism started. She has h...

ea0041ep42 | Adrenal cortex (to include Cushing's) | ECE2016

What can a tissue measured steroid metabolome tell about adrenal tumor? A tissue steroid analysis of cortisol producing adenoma and an androgen producing adenoma in a second patient with CAH, 21-hydroxylase deficiency

Hana Vaclav , Kosak Mikulas , Michalsky David , Hana Vaclav , Hill Martin

Introduction: A key feature of a benign adrenal tumor is its hormonal production. It is reflected by serum steroid levels. A tumoral tissue steroid content is not commonly measured.Methods: A steroid metabolome comprising of 65 steroids was measured in four samples from each of two patients using GC-MS in serum before and after 1mg dexamethasone test, in the tumoral tissue and in the adrenal tissue of two female patients with adrenal adenomas. The first ...

ea0032p225 | Clinical case reports – Pituitary/Adrenal | ECE2013

Delivery of health child in acromegaly patient with McCune–Albright syndrome treated with lanreotide and pegvisomant during pregnancy

Weiss Vladimir , Hana Vaclav , Marek Josef

Introduction: Acromegaly with GH excess affects up to 20% of the patients with McCune–Albright syndrome (MAS). Surgical treatment for acromegaly in MAS is often difficult because of skull-base dysplasia. Somatostatin analogs are frequently only partially effective and GH receptor antagonist – pegvisomant is more potent in normalizing IGFI levels. Radiotherapy is controversial. Pregnancy in MAS patients is described in literature but no case of successful delivery in ...

ea0056p161 | Steroid metabolism + action | ECE2018

Steroid profile using gas chromatography tandem mass spectrometry (GC-MS/MS) in search for a steroid which correlates most with subclinical hypercortisolism

Hana jr. Vaclav , Kosak Mikulas , Hana Vaclav , Hill Martin

Introduction: Gas chromatography tandem mass spectrometry (GC-MS/MS) quantitatively detects a large number of steroids at one time in a single serum sample. Most studies of subclinical hypercortisolism in adrenal incidentalomas have focused on detection of a few steroids like cortisol, DHEA/S, androstenedione, testosterone. We used GC-MS/MS to quantify 91 steroids in patients with adrenal incidentalomas to search for the most reliable marker of subclinical hypercortisolism.</p...

ea0037ep751 | Pituitary: clinical | ECE2015

Endonasal endoscopic pituitary adenoma resection

Masopust Vaclav , Netuka David , Benes Vladimir , Bradac Ondrej , Marek Josef , Hana Vaclav , Krsek Michal

Introduction: In the past 10 years, endoscopic resection of pituitary adenomas has become an alternative to microsurgical resection with the additional advantage of increasing the patient’s postoperative comfort. This analysis explored whether endoscopic resection can reduce the risk of postoperative neurohypophyseal dysfunction.Material and methods: We rated and compared the need to administer desmopressin during the first four postoperative days a...

ea0032p31 | Adrenal cortex | ECE2013

Reliability of serum versus salivary cortisol in ACTH test

Kosak Mikulas , Hana Vaclav , Hill Martin , Simunkova Katerina , Lacinova Zdena , Krsek Michal , Marek Josef

Introduction: Salivary cortisol measurement, representing free cortisol, seems to be a promissing alternative method to serum cortisol. Besides its use in the diagnostics of hypercortisolism, it could be of help in evaluation of adrenocortical reserve. It might be useful especially in patients on estrogen replacement, which modulates transcortin and hence total cortisol levels.Aims: Compare the reliability of salivary vs. serum cortisol assessment during...

ea0022p595 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Pituitary functions in patients with chronic subdural hematoma

Kosak Mikulas , Masopust Vaclav , Netuka David , Lacinova Zdenka , Krsek Michal , Marek Josef , Hana Vaclav

Certain degree of hypopituitarism has been revealed in a significant number of patients who suffered traumatic brain injuries or subarachnoid hemorrhage. Microhemorrhages, necrosis, tissue infarcts and vasoconstriction are reported as mechanism of hypothalamo-pituitary dysfunction. Assessment of hypothalamo-pituitary endocrine functions in patients with chronic subdural hematomas has not been published yet, although dysfunction of hypothalamo-pituitary unit can be expected (he...

ea0020p491 | Obesity and Metabolism | ECE2009

Regulation of novel metabolic regulator fibroblast growth factor-21 by body adiposity and hypercortisolemia: studies in patients with obesity and Cushing’s syndrome

Durovcova Viktoria , Krsek Michal , Hana Vaclav , Marek Josef , Bartlova Marketa , Kavalkova Petra , Haluzik Martin

Fibroblast growth factor-21 (FGF-21) is a novel regulator of metabolic homeostasis that improved diabetes compensation and dyslipidemia in diabetic mice and monkeys. However, little is known about its regulation in humans. Cushing’s syndrome (CS) is characterized by endogenous hypercortisolism and is often associated with numerous metabolic abnormalities. The objective of this study was to test the hypothesis that CS is associated with altered levels of FGF 21 that may in...