Searchable abstracts of presentations at key conferences in endocrinology

ea0065bpw2.2 | Imaging endocrinology from networks to organelles | SFEBES2019

Spatial programming of GPCR signalling

Hanyaloglu Aylin

The spatial organisation of receptors at the macro and micro-scale is critical for the tight regulation of cell signalling, including signalling activated by the superfamily of G protein-coupled receptors (GPCRs). To study these processes, a range of imaging techniques have been employed including an increasing application of super-resolution microscopy to image receptor activity beyond the diffraction limit of light. In this session, I will highlight the experimental and imag...

ea0041s20.1 | Hitchhiker's guide to the microsmos of GPCRs | ECE2016

Location, location, location: spatial programming of GPCR signalling

Hanyaloglu Aylin

An archetypal view of GPCR signalling depicts cell surface localized receptors activating specific heterotrimeric G-protein signal pathways, which in turn converge on to common downstream pathways. How such signals are translated into the highly diverse cellular and physiological responses that are controlled by this superfamily of receptors has been a long-standing biological question. This question has driven our more current understanding of the complexity of these receptor...

ea0025ye1.3 | A successful research career | SFEBES2011

Postdoctoral research – the things I wish I knew

Hanyaloglu Aylin

Many PhD graduates will pursue a postdoctoral training position, most commonly in an academic laboratory. What can you expect from this period in your career? Do you need to go abroad to do a postdoc? What could/should you do to benefit the most of this time? And how can you use your time as a postdoc to help you with your career path once you leave?I recall being very excited about beginning my postdoc and leaving the ‘PhD nest’. I trained and...

ea0086ec1.4 | Clinical Endocrinology Trust Best Abstract Basic | SFEBES2022

Characterising the G-protein signalling mechanisms activated by short chain fatty acids in human enteroendocrine cells which mediate production and release of the anorectic gut hormones peptide YY (PYY) and glucagon-like peptide-1 (GLP-1)

Hirdaramani Aanya , Frost Gary , Hanyaloglu Aylin

The anorectic gut hormones glucagon-like peptide-1 (GLP-1) and Peptide YY (PYY) are secreted by enteroendocrine L cells in response to short chain fatty acids (SCFAs) produced by gut microbiota following dietary fibre consumption. SCFAs, acetate, propionate and butyrate, activate the G protein-coupled receptors Free Fatty Acid Receptor 2 and 3 (FFAR2, FFAR3). Increasing intestinal levels of propionate can prevent weight gain in overweight adults. In rodents, FFAR2-mediated gut...

ea0038p349 | Reproduction | SFEBES2015

Decoding gonadotrophin receptor signalling via spatial regulation of the LH receptor

Sposini Silvia , Jean-Alphonse Frederic , Hanyaloglu Aylin

The LH receptor (LHR) is essential for mediating multiple functions in reproduction and pregnancy. LHR belongs to the superfamily of G-protein-coupled receptors (GPCRs) that impacts nearly every aspect of human physiology and pathophysiology. Membrane trafficking is a critical mechanism for cells to decode complex signalling networks into specific downstream responses, including the signalling pathways activated by GPCRs. A detailed molecular description of GPCR trafficking is...

ea0031p296 | Pituitary | SFEBES2013

Dissecting the prokineticin receptor dimerization interface: a role in kallmann sindrome?

Sposini Silvia , Hanyaloglu Aylin , Miele Rossella

Prokineticin receptors (PKR1 and PKR2) are GPCRs that belong to neuropeptide Y receptor class. They exert their biological functions binding two structurally related peptides (Bv8 or PK2 and EG-VEGF orr PK1). Intensive research over the past few years has shown that PKs/PKRs signalling modulates neuronal survival and neurogenesis, hypothalamic hormone secretion, nociception, circadian rhythm and complex behaviours, such as feeding and drinking. It also promotes angiogenesis in...

ea0031p306 | Pituitary | SFEBES2013

Regulation of LH/CG receptor signaling in human endometrium and perturbations in recurrent pregnancy loss

West Camilla , Hanyaloglu Aylin , Brosens Jan

G-protein coupled receptors (GPCRs) represent the largest family of signaling receptors in nature. Their diversity means they play key physiological roles and their dysfunction underlies many pathological conditions, thus they are the focus of many drug design programs due to their primary biological and clinical importance. Our objective is to understand the fundamental mechanisms regulating hormone signaling via GPCRs. Disruption of GPCRs regulation in humans underlies many ...

ea0086op4.2 | Metabolism, Obesity and Diabetes | SFEBES2022

Differential effects of L- and D-lactate on HCAR1 signalling

Milner Annabelle , Brown Alastair , Frost Gary , Hanyaloglu Aylin

Lactate is a metabolite that activates the G-protein coupled receptor, Hydroxycarboxylic acid Receptor 1 (HCAR1) to regulate physiological processes such as lipolysis, cancer cell survival, and neuroprotection. Lactate exists in two forms, L+ and D-, with the L isoform predominant in the human body. Interestingly, both isoforms are only found together in the gastrointestinal tract. L-lactate is synthesised as a by-product of anaerobic respiration, whereas D-lactate, is a produ...

ea0086p119 | Reproductive Endocrinology | SFEBES2022

FSH glycosylation variants differentially modulate FSHR trafficking

Agwuegbo Uche , Richardson Rachel , Albert Anthony , Hanyaloglu Aylin , Jonas Kim

The Class A G protein-coupled receptor (GPCR), follicle-stimulating hormone receptor (FSHR), and its associated heterodimeric glycoprotein hormone (FSH) are essential for reproduction. As such, they’re key targets of assisted conception. Post-translational modification of FSH gives rise to two predominant glycosylation variants, which are modulated with ageing: partially glycosylated FSH (FSH21/18), predominates in women’s reproductive prime (20’s), displays fas...

ea0086p124 | Reproductive Endocrinology | SFEBES2022

Divergent G-protein signal control at the very early endosome (VEE) from the dually coupled luteinizing hormone receptor (LHR)

Richardson Rachel , Sposini Silvia , Yildiz Yoldas , Barlow Lucy , Hanyaloglu Aylin

Membrane trafficking of G-protein coupled receptors (GPCRs) is a critical mechanism by which cells mediate complex signaling pathways. Endosomes are increasingly recognized as platforms for GPCR signaling. The VEE was first identified using the LHR, a GPCR that is key in reproduction. We have shown that recycling of the LHR is driven by receptor mediated Gαs cAMP signaling from the VEE and PKA-dependent phosphorylation of the adaptor protein, APPL1, while APPL1 negatively...