Searchable abstracts of presentations at key conferences in endocrinology

ea0015oc13 | Reproduction | SFEBES2008

Effect of hypoxia and inflammatory and anti-inflammatory signals on gene expression in sheep ovarian surface epithelium in vitro

Harlow Christopher R , Rae Michael , Price Deborah , McNeilly Alan S , Rhind Stewart M , Hillier Stephen G

The intraperitoneal environment of the ovarian surface epithelium (OSE) has an oxygen (O2) tension of 5–12%. This could impact cellular events leading to ovulation. Furthermore, ovarian cancers arising from the OSE (∼80–90% of ovarian malignancies) are likely to experience increased hypoxia due to the relatively poor vascularisation of solid tumours.Using 1%, 5% or 20% O2, we measured the expression of HSD11B1, COX-2...

ea0015p185 | Endocrine tumours and neoplasia | SFEBES2008

Steroid Sulphatase (STS) gene expression and regulation in normal human ovarian surface epithelium (OSE) and epithelial ovarian cancer (EOC)

Harlow Christopher R , Wu Xuan , Ren Xia , Fegan Scott , Rae Michael , Price Deborah , Mason J Ian , Hillier Stephen G

At least 90% of human ovarian cancers originate in the OSE. Most studies indicate that EOC is oestrogen responsive. Paradoxically, ovarian cancer generally occurs after the menopause, which raises the question if oestrogen is involved, where does it come from? We hypothesise that the high circulating concentrations of conjugated (inactive) oestrogens in post-menopausal women are substrates for formation of active oestrogen in the OSE through the hydrolytic enzyme activity of S...

ea0034oc3.6 | Steroids | SFEBES2014

Targeting of lysyl oxidase by steroids to reduce peritoneal fibrosis and scarring

Harlow Christopher , Wu Xuan , van Deemter Marielle , Gardiner Fiona , Poloand Craig , Green Rebecca , Sarvi Sana , Kadler Karl , Lu Yinhui , Mason J Ian , Critchley Hilary , Hillier Stephen

Abdominal surgery and diseases such as endometriosis cause persistent abdominal adhesions leading to pelvic pain, infertility and in severe cases, bowel obstruction. Current treatments are ineffective and the aetiology is unclear, although excessive collagen deposition is a consistent feature. Lysyl oxidase (LOX) is a key enzyme required for crosslinking and deposition of insoluble collagen.10–12 weeks old female C57Bl/6 mice (3–10 mice per gro...