Searchable abstracts of presentations at key conferences in endocrinology

ea0077p59 | Metabolism, Obesity and Diabetes | SFEBES2021

Acidosis reduces 11β-HSD1 activity in human primary muscle cell cultures

Sagmeister Michael , Nicholson Thomas , Harper Lorraine , Jones Simon , Hardy Rowan

Background: Acidosis activates the hypothalamic-pituitary-adrenal (HPA) axis and induces glucocorticoid-mediated atrophy of skeletal muscle. The enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) converts inactive cortisone to active cortisol and modulates glucocorticoid signalling locally within skeletal muscle. Here, we address a gap in knowledge how acidosis affects 11β-HSD1 activity in human skeletal muscle cells.Methods: Quadrice...

ea0086p59 | Metabolism, Obesity and Diabetes | SFEBES2022

The role of glucocorticoid activation by 11bHSD1 for muscle wasting in a mouse model of renal impairment

Sagmeister Michael , Crastin Ana , Jones Simon , Harper Lorraine , Hardy Rowan

Background: Chronic kidney disease aggravates loss of skeletal muscles mass and function, which is an independent risk factor for hospitalisation, morbidity and mortality. Glucocorticoid signalling has been implicated as a critical factor in the pathogenesis of muscle atrophy in kidney disease. This study tests whether genetic deletion of the glucocorticoid-activating enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11bHSD1) protects against muscle atrophy in the adenine-die...

ea0094oc1.3 | Bone and Calcium | SFEBES2023

The role of glucocorticoid metabolism in the skeletal pathophysiology of chronic kidney disease

Shanker Arjan , Cassidy Ben , Crastin Ana , Poologasundarampillai Gowsihan , Hardy Rowan , Harper Lorraine , Jones Simon W. , Sagmeister Michael

Background: Osteoporosis is a common feature of chronic kidney disease (CKD), associated with premature mortality. Glucocorticoids (GCs) are steroid hormones, that in excess, can drive the suppression of bone formation. We have shown that the glucocorticoid (GC) activating enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is dysregulated in CKD, where it may contribute to the suppression of bone formation. We utilised a murine model of CKD with...

ea0094p210 | Metabolism, Obesity and Diabetes | SFEBES2023

The contribution of glucocorticoid metabolism by 11β-HSD1 towards muscle wasting in chronic kidney disease

Cassidy Ben , Firkins Louise , Sagmeister Michael , Crastin Ana , Poologasundarampillai Gowsihan , Harper Lorraine , W. Jones Simon , S. Hardy Rowan

Background: Skeletal muscle wasting is a characteristic feature of chronic kidney disease (CKD), associated with increased hospitalisations and premature mortality. Excess glucocorticoid signalling is a major contributor to the pathogenesis of muscle wasting in conditions of renal impairment. This study aimed to validate a murine model of CKD and utilise this to determine if deletion of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) provides protec...