ea0005p209 | Reproduction | BES2003
Sorsa-Leslie T
, Harris W
, Mason H
, Fowler P
Objective We aimed to immunopurify GnSAF, using antibodies with demonstrated GnSAF bioactivity-blocking/binding activity, in order to confirm 2 new candidate amino acid sequences for human ovarian GnSAF (Fowler et al. Mol Human Reprod 2002;8:823-832).Methods Rat antiserum, raised against a 60-70 kDa band of partially purified GnSAF, was immobilised on anti-rat IgG Dynabeads. In addition, a human antibody, derived from a phage display antibody library a...