Searchable abstracts of presentations at key conferences in endocrinology

ea0031p179 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

FGF21 action on human adipose tissue compromised by reduced βKlotho and FGFR1 expression in type 2 diabetes mellitus

Piya Milan K , Harte Alison L , Chittari Madhu V , Tripathi Gyanendra , Kumar Sudhesh , McTernan Philip G

Background/objectives: Fibroblast growth factor 21 (FGF21) is a potent hormone known to reduce glycaemia and improve insulin resistance with anti-obesity effects. Although mainly secreted in the liver, adipose tissue is considered an important target for its function. Whilst FGF21 has been shown to be expressed in murine adipose tissue, studies in human adipose tissue are lacking. Therefore our aim was to examine the expression of FGF21, FGF21 receptor-1 (FGFR1), βKlotho ...

ea0031p201 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Unfolded protein response pathway, IRE1α-XBP1 is altered during adipogenesis in obese human adipocytes

Voyias Philip , McCarthy Ciara , Antonysunil Adaikala , Kumsaiyai Warunee , Harte Alison , McTernan Philip , Tripathi Gyanendra

Background: Adipose tissue (AT) plays a central role in obesity-related complications such as cardiovascular diseases, insulin resistance, and type two diabetes. A comprehensive understanding of the molecular mechanisms underlying adipocyte formation is of both fundamental and clinical relevance. It has been reported that UPR pathway, IRE1α-XBP1 regulates hepatic lipogenesis and the role of this pathway in adipogenesis in murine adipocytes has also been confirmed. Our tea...

ea0031p204 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Altered mitochondrial dynamics in obesity: redressing the balance through bariatric surgery?

McCarthy Ciara , Voyias Philip , Antonysunil Adaikala , Harte Alison , Saravanan Ponnusamy , Kyrou Ioannis , Tripathi Gyanendra , McTernan Philip

Mitochondria are essential for synthesising ATP required for cellular metabolism. Mitochondria are able to alter their morphology and abundance through the balance of fission and fusion genes. T2DM is often accompanied by a metabolic syndrome within the individual. The aim of my PhD is to investigate whether metabolic dysregulation in T2DM can be linked to dysfunction within their mitochondria.Declaration of funding: EPSRC....

ea0028p213 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Chronic or intermittent exposure to Oleic acid (MUFA) induces a greater NFκB activated inflammatory response than Eicosapentaenoic acid (PUFA) in differentiated human adipocytes

Piya Milan , Harte Alison , McGee Kirsty , Reddy Narendra , Tripathi Gyanendra , McTernan Philip , Kumar Sudhesh

Replacement with mono-unsaturated fatty acids (MUFA) or poly-unsaturated fatty acids (PUFA) reduces inflammation in the pre-diabetic subjects but the benefits are unclear in subjects with type 2 diabetes mellitus (T2DM). Our aim was to investigate whether saturated fatty acids (SFA), MUFA and PUFA produce a similar adipocyte inflammatory response, which may explain the rationale for maintained systemic inflammation noted in subjects with T2DM. The differentiated human preadipo...

ea0021p137 | Diabetes and metabolism | SFEBES2009

Salicylate induces insulin sensitivity by alleviating endoplasmic reticulum (ER) stress in cultured primary human adipocytes

Tripathi Gyanendra , Alhusaini Saif , McGee Kirsty , Lois Kostantinos , Harte Alison , McTernan Philip , Kumar Sudhesh

Background: Adipose tissue (AT) may have a significant role in obesity associated inflammation but, the mechanisms underlying the pathogenesis of obesity induced inflammation remains unclear. Recent murine studies indicate that ER stress is critical to the initiation and integration of inflammation and insulin signalling pathways. The ER stress occurs primarily due to accumulation of unfolded proteins and results in activation of the unfolded protein response to restore ER hom...

ea0014p222 | (1) | ECE2007

Human adipose tissue derived DPP-IV regulates lipolysis through NPY in cultured abdominal subcutaneous adipocytes

Kos Katarina , Baker Adam , Harte Alison , McTernan Philip , O’Hare Paul , Kumar Sudhesh

We have previously shown that the orexigenic hormone NPY is secreted by human adipocytes. The orexigenic hormone NPY(1–36) is truncated by the dipeptidyl-inhibitor IV (DPP-IV) to NPY(3–36) as consequence its affinity changes from receptor Y1 to Y4 and 5. The aim was to investigate whether DPP-IV is expressed in adipose tissue (AT) where it could modulate adipose tissue growth through modulation of NPY activity. This is relevant in light of DPP-IV inhibitors utilised ...

ea0031p13 | Bone | SFEBES2013

Identification of a novel heterozygous mutation in exon 50 of the COL1A1 gene manifesting clinically as osteogenesis imperfecta

Owen Nina , Reddy Narendra , Aftab Saboor AS , Harte Alison L , McTernan Philip G , Tripathi Gyanendra , Barber Thomas M

Introduction: Osteogenesis imperfecta (OI) is a rare, heterogeneous, genetic connective tissue disorder that manifests clinically as bone fragility, brittleness and growth disorder. Effective diagnosis is important (although often challenging) to enable institution of early and effective multidisciplinary management.The case: A 19-year-old woman was referred to the Endocrine clinic at the Warwickshire Institute for the Study of Diabetes, Endocrinology an...

ea0031p188 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Human abdominal subcutaneous adipocytes as an active source of LpPLA2, influenced by fat depot and metabolic state, with LpPLA2 converting LDL into more potent atherogenic Ox-LDL, in vitro

Kumsaiyai Warunee , Harte Alison , Al-Naji Fadi , Al-Daghri Nasser , Kyrou Ioannis , Barber Thomas , Sabico Shaun , Tripathi Gyanendra , McTernan Philip

Lipoprotein-associated phospholipase A2 (LpPLA2) is a member of the phospholipaseA2 super family of enzymes, and is upregulated in arterial inflammation, obesity and cardiovascular disease. The other isoforms, iPLA2 and cPLA2, appear to contribute to inflammation through production of lipid mediators. The role of PLA2 in human adipose tissue (AT) is unclear, therefore we sought to i) characterise PLA2 isoforms in lean, obese, T2DM abdominal subcutaneous (AbdSc) and omental (Om...

ea0031p205 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Irisin as a central regulator in energy homeostasis?

Piya Milan K , Harte Alison L , Sukumar Kavitha , James Sean , Tripathi Gyanendra , Kumar Sudhesh , Vatish Manu , McTernan Philip G

Background/objectives: Irisin is a novel myokine, released predominantly by skeletal muscle. Peripheral action of irisin improves glucose homeostasis, increases energy expenditure and induces browning of adipocytes, with therapeutic potential for use in weight loss. However, there are no human studies on the central role of irisin in the regulation of appetite or energy expenditure. This study sought to examine the potential central role of irisin, by demonstrating irisin in h...

ea0028p176 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Expression of genes regulating mitochondrial fusion and fission in human adipose tissue are influenced by adiposity and bariatric surgery

McCarthy Ciara , Voyias Philip , Antonysunil Adaikala , McGee Kirsty , Harte Alison , Saravanan Ponnusamy , Kyrou Ioannis , Kumar Sudhesh , McTernan Philip , Tripathi Gyanendra

Mitochondria are essential for synthesising ATP required for cellular metabolism. In response to changes in their cellular environment, mitochondria are able to alter their morphology and abundance through the balance of “fusion” and “fission” events. The aim of this study was to profile the expression of mitochondrial genes in the abdominal subcutaneous (AbSc) adipose tissue (AT) of lean, obese and Type 2 Diabetes Mellitus (T2DM) individuals and determine ...