Searchable abstracts of presentations at key conferences in endocrinology

ea0099rc13.4 | Rapid Communications 13: Late Breaking | ECE2024

Development and validation of an LC-MS/MS method to measure 7-alpha-hydroxy-4-cholesten-3-one (C4) for the assessment of bile acid dysregulation in PCOS

Atkins Jonathan S , Keevil Brian , Ludwig Christian , Hawley James M.

Introduction: 7α-Hydroxy-4-cholesten-3-one (C4) is the common intermediary of the primary bile acids (BAs). Its concentration in serum correlates with the rate of activity of hepatic CYP7A1 and therefore with the rate of hepatic BA production. Previous work has smggested a role for BA pool dysregulation in the metabolic syndrome and gut dysbiosis seen in polycystic ovary syndrome (PCOS). However, the mechanism of this is still to be elucidated. Here we report a novel LC-M...

ea0081ep105 | Adrenal and Cardiovascular Endocrinology | ECE2022

Serum cortisol immunoassay performance in the overnight dexamethasone suppression test.

Atkins Jonathan , Owen Laura , Clayton Jonathan , Hawley James M , Scargill Jonathan , Keevil Brian

Background: The 1 mg overnight dexamethasone suppression test (ONDST) is recommended for the differential diagnosis of Cushing’s syndrome and the investigation of adrenal incidentalomas. However, diagnostic performance of the test relies on accurate methods to quantitate cortisol in serum. Although the variable performance of serum cortisol immunoassays has been well-documented, little has been published on their performance following the ONDST.Aims...

ea0081p27 | Adrenal and Cardiovascular Endocrinology | ECE2022

Multiplex serum steroid profiling using liquid chromatography mass spectrometry with post column infusion ammonium fluoride

Schiffer Lina , Shaheen Fozia , Gilligan Lorna , Storbeck Karl , Hawley James M , Keevil Brian , Arlt Wiebke , Taylor Angela

Background: Development of multi-steroid profiling allows comprehensive investigation into the different branches of steroid metabolism. Immunoassays only allow analysis of a single steroid per assay and suffer from problems with specificity due to cross reactivity of similar steroids. Liquid-chromatography mass spectrometry has the specificity to analyse multiple steroids in a single experiment and the dynamic range to quantify steroids at high concentrations such as those ob...

ea0081ep879 | Reproductive and Developmental Endocrinology | ECE2022

Daytime salivary androgen rhythm by LC-MS/MS in women affected by Polycystic Ovary Syndrome (PCOS) fulfilling the three Rotterdam diagnostic criteria

Fanelli Flaminia , Hawley James M , Mezzullo Marco , Fazzini Alessia , Zanotti Laura , Magagnoli Matteo , Pagotto Uberto , Keevil Brian , Gambineri Alessandra

Background: Excess testosterone in PCOS is accompanied by increased levels of other ovarian and adrenal androgens and precursors. Recently, 11-oxygenated adrenal androgens have been postulated as major components of the circulating androgen pool in PCOS. Obesity is strictly connected with androgen excess and with the derangement of hormone circadian rhythmicity. To date, it has not been clarified whether hyperandrogenism in PCOS, either complicated by obesity or not, is accomp...

ea0081oc13.3 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

11-oxygenated C19 steroids are the predominant androgens responsible for hyperandrogenemia in Cushing’s disease

Nowotny Hanna , Vogel Frederick , Bidlingmaier Martin , Braun Leah , Reincke Martin , Tschaidse Lea , Auer Matthias , Lottspeich Christian , Hawley James M , Adaway Jo , Keevil Brian , Schilbach Katharina , Reisch Nicole

Background: Symptoms of hyperandrogenism are common in patients with Cushing’s disease (CD), but they cannot be sufficiently explained by measured concentrations of circulating androgens. In this study we analyzed the contribution of 11-oxygenated (11o×C19) androgens to hyperandrogenemia in female patients with CD as well as the influence of treatment with steroidogenesis inhibitors osilodrostat and metyrapone on 11o×C19 and classic androgens.<p class="abste...

ea0081oc13.4 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

Classic and 11-oxygenated androgens in serum and saliva across adulthood and the menstrual cycle – a mass spectrometry-based cross-sectional study

Kempegowda Punith , Schiffer Lina , Adaway Jo , Shaheen Fozia , Ebbehoj Andreas , Singh Sumitabh , Prete Alessandro , Hawley James M , Sitch Alice J , Keevil Brian , Bancos Irina , Taylor Angela , Arlt Wiebke

Objective: Quantify classic and 11-oxygenated androgens in serum and saliva and determine variations across age, sex, body mass index (BMI), menstruation and hormonal contraception use.Methods: Morning serum samples were collected from 292 healthy volunteers (125 men, 22-95 years; 167 women, 21-91 years). Morning saliva was collected from 83 healthy volunteers (51 women, 32 men). 25 individuals (12 women, 13 men) also collected a 7-timepoint diurnal sali...

ea0073pep1.1 | Presented ePosters 1: Adrenal and Cardiovascular Endocrinology | ECE2021

Salivary steroid and 11‑oxygenated androgen profiles in patients with congenital adrenal hyperplasia on various glucocorticoid replacement regimens

Auer Matthias , Nowotny Hanna , Quinkler Marcus , Bidlingmaier Martin , Hawley James M , Adaway Jo , Keevil Brian , Ross Richard , Porter John , Reisch Nicole

Context11-oxygenated C19 steroids have recently gained attention as markers of androgen control in congenital adrenal hyperplasia (CAH) due to 21hydroxylase deficiency (21OHD). However, they have not yet been systematically investigated in the context of different glucocorticoid (GC) replacement regimens and in particular not in patients receiving new modified-release formulations.MethodsCross-sectional singl...

ea0073pep1.5 | Presented ePosters 1: Adrenal and Cardiovascular Endocrinology | ECE2021

Modified-release hydrocortisone improves androgen excess and facilitates glucocorticoid dose reduction in patients with classic congenital adrenal hyperplasia: non-invasive monitoring in saliva and urine

Prete Alessandro , Baranowski Elizabeth S. , Lina Schiffer , Adaway Joanne E. , Hawley James M. , Keevil Brian G. , Porter John , Ross Richard J. , Arlt Wiebke

BackgroundStandard glucocorticoid (GC) therapy in classic congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21-OHD-CAH) is often inadequate in controlling adrenal androgen excess, leading to GC over-exposure and poor health outcomes. A novel modified-release formulation of hydrocortisone (MR-HC, Chronocort® Diurnal Ltd. UK) has been shown to improve circulating adrenal androgen excess in 21-OHD-CAH. We investigated whether saliva and ...

ea0099p237 | Adrenal and Cardiovascular Endocrinology | ECE2024

Fertility outcomes in women with classic and non-classic congenital adrenal hyperplasia on modified-release hydrocortisone (MR-HC)

Tschaidse Lea , Nowotny Hanna F , Auer Matthias , Lottspeich Christian , Welp Ann-Christin , Bidlingmaier Martin , Hawley James M , Keevil Brian , Reisch Nicole

Background: Women with classic and non-classic (NC) congenital adrenal hyperplasia (CAH) often struggle with fertility, due to androgen excess, elevated progesterone and 17-hydroxyprogesterone (17OHP) levels, causing anovulation, impaired endometrial development and menstrual irregularities. The typical approach to addressing infertility so far, often involves an increase in glucocorticoid (GC) doses, yet this strategy carries potential side effects. Limited observational data...

ea0081p386 | Endocrine-Related Cancer | ECE2022

Urinary steroid metabolomics for adrenocortical cancer diagnosis. Comparison of gas chromatography mass spectrometry to liquid chromatography mass spectrometry

Taylor Angela , Bancos Irina , Gilligan Lorna , van Veen Rick , Chortis Vasileios , Shaheen Fozia , Jenkinson Carl , O'Neil Donna M , Hughes Beverly , Hawley James M , Keevil Brian , Shackelton Cedric H L , Deeks Jonathan , Sitch Alice J , Biehl Michael , Arlt Wiebke

Introduction: Gas chromatography mass spectrometry (GC-MS) is the gold standard method for urinary steroid profiling. However, GC-MS requires chemical derivatisation, long run times, is labour intensive, expensive, and unsuitable for rapid multi-sample analysis, limiting its use in routine clinical practice. GC-MS urinary steroid metabolomics, the combination of steroid profiling and machine learning (Generalized Matrix Learning Vector Quantization) was shown to have superior ...