Searchable abstracts of presentations at key conferences in endocrinology

ea0081ep105 | Adrenal and Cardiovascular Endocrinology | ECE2022

Serum cortisol immunoassay performance in the overnight dexamethasone suppression test.

Atkins Jonathan , Owen Laura , Clayton Jonathan , Hawley James M , Scargill Jonathan , Keevil Brian

Background: The 1 mg overnight dexamethasone suppression test (ONDST) is recommended for the differential diagnosis of CushingÂ’s syndrome and the investigation of adrenal incidentalomas. However, diagnostic performance of the test relies on accurate methods to quantitate cortisol in serum. Although the variable performance of serum cortisol immunoassays has been well-documented, little has been published on their performance following the ONDST.Aims...

ea0081p27 | Adrenal and Cardiovascular Endocrinology | ECE2022

Multiplex serum steroid profiling using liquid chromatography mass spectrometry with post column infusion ammonium fluoride

Schiffer Lina , Shaheen Fozia , Gilligan Lorna , Storbeck Karl , Hawley James M , Keevil Brian , Arlt Wiebke , Taylor Angela

Background: Development of multi-steroid profiling allows comprehensive investigation into the different branches of steroid metabolism. Immunoassays only allow analysis of a single steroid per assay and suffer from problems with specificity due to cross reactivity of similar steroids. Liquid-chromatography mass spectrometry has the specificity to analyse multiple steroids in a single experiment and the dynamic range to quantify steroids at high concentrations such as those ob...

ea0081ep879 | Reproductive and Developmental Endocrinology | ECE2022

Daytime salivary androgen rhythm by LC-MS/MS in women affected by Polycystic Ovary Syndrome (PCOS) fulfilling the three Rotterdam diagnostic criteria

Fanelli Flaminia , Hawley James M , Mezzullo Marco , Fazzini Alessia , Zanotti Laura , Magagnoli Matteo , Pagotto Uberto , Keevil Brian , Gambineri Alessandra

Background: Excess testosterone in PCOS is accompanied by increased levels of other ovarian and adrenal androgens and precursors. Recently, 11-oxygenated adrenal androgens have been postulated as major components of the circulating androgen pool in PCOS. Obesity is strictly connected with androgen excess and with the derangement of hormone circadian rhythmicity. To date, it has not been clarified whether hyperandrogenism in PCOS, either complicated by obesity or not, is accomp...

ea0081oc13.3 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

11-oxygenated C19 steroids are the predominant androgens responsible for hyperandrogenemia in Cushing’s disease

Nowotny Hanna , Vogel Frederick , Bidlingmaier Martin , Braun Leah , Reincke Martin , Tschaidse Lea , Auer Matthias , Lottspeich Christian , Hawley James M , Adaway Jo , Keevil Brian , Schilbach Katharina , Reisch Nicole

Background: Symptoms of hyperandrogenism are common in patients with CushingÂ’s disease (CD), but they cannot be sufficiently explained by measured concentrations of circulating androgens. In this study we analyzed the contribution of 11-oxygenated (11o×C19) androgens to hyperandrogenemia in female patients with CD as well as the influence of treatment with steroidogenesis inhibitors osilodrostat and metyrapone on 11o×C19 and classic androgens.<p class="abste...

ea0081oc13.4 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

Classic and 11-oxygenated androgens in serum and saliva across adulthood and the menstrual cycle – a mass spectrometry-based cross-sectional study

Kempegowda Punith , Schiffer Lina , Adaway Jo , Shaheen Fozia , Ebbehoj Andreas , Singh Sumitabh , Prete Alessandro , Hawley James M , Sitch Alice J , Keevil Brian , Bancos Irina , Taylor Angela , Arlt Wiebke

Objective: Quantify classic and 11-oxygenated androgens in serum and saliva and determine variations across age, sex, body mass index (BMI), menstruation and hormonal contraception use.Methods: Morning serum samples were collected from 292 healthy volunteers (125 men, 22-95 years; 167 women, 21-91 years). Morning saliva was collected from 83 healthy volunteers (51 women, 32 men). 25 individuals (12 women, 13 men) also collected a 7-timepoint diurnal sali...

ea0073pep1.1 | Presented ePosters 1: Adrenal and Cardiovascular Endocrinology | ECE2021

Salivary steroid and 11‑oxygenated androgen profiles in patients with congenital adrenal hyperplasia on various glucocorticoid replacement regimens

Auer Matthias , Nowotny Hanna , Quinkler Marcus , Bidlingmaier Martin , Hawley James M , Adaway Jo , Keevil Brian , Ross Richard , Porter John , Reisch Nicole

Context11-oxygenated C19 steroids have recently gained attention as markers of androgen control in congenital adrenal hyperplasia (CAH) due to 21hydroxylase deficiency (21OHD). However, they have not yet been systematically investigated in the context of different glucocorticoid (GC) replacement regimens and in particular not in patients receiving new modified-release formulations.MethodsCross-sectional singl...

ea0073pep1.5 | Presented ePosters 1: Adrenal and Cardiovascular Endocrinology | ECE2021

Modified-release hydrocortisone improves androgen excess and facilitates glucocorticoid dose reduction in patients with classic congenital adrenal hyperplasia: non-invasive monitoring in saliva and urine

Prete Alessandro , Baranowski Elizabeth S. , Lina Schiffer , Adaway Joanne E. , Hawley James M. , Keevil Brian G. , Porter John , Ross Richard J. , Arlt Wiebke

BackgroundStandard glucocorticoid (GC) therapy in classic congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21-OHD-CAH) is often inadequate in controlling adrenal androgen excess, leading to GC over-exposure and poor health outcomes. A novel modified-release formulation of hydrocortisone (MR-HC, Chronocort® Diurnal Ltd. UK) has been shown to improve circulating adrenal androgen excess in 21-OHD-CAH. We investigated whether saliva and ...

ea0081p386 | Endocrine-Related Cancer | ECE2022

Urinary steroid metabolomics for adrenocortical cancer diagnosis. Comparison of gas chromatography mass spectrometry to liquid chromatography mass spectrometry

Taylor Angela , Bancos Irina , Gilligan Lorna , van Veen Rick , Chortis Vasileios , Shaheen Fozia , Jenkinson Carl , O'Neil Donna M , Hughes Beverly , Hawley James M , Keevil Brian , Shackelton Cedric H L , Deeks Jonathan , Sitch Alice J , Biehl Michael , Arlt Wiebke

Introduction: Gas chromatography mass spectrometry (GC-MS) is the gold standard method for urinary steroid profiling. However, GC-MS requires chemical derivatisation, long run times, is labour intensive, expensive, and unsuitable for rapid multi-sample analysis, limiting its use in routine clinical practice. GC-MS urinary steroid metabolomics, the combination of steroid profiling and machine learning (Generalized Matrix Learning Vector Quantization) was shown to have superior ...

ea0063p1034 | Interdisciplinary Endocrinology 2 | ECE2019

Harmonization status among ten European laboratories using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for steroid measurements in serum: preliminary results from the HarmoSter consortium

Fanelli Flaminia , Mezzullo Marco , Temchenko Anastasia , Cantu Marco , Lindner Johanna M , Peitzsch Mirko , Hawley James M , Bruce Stephen , Zelzer Sieglinde , Herrmann Markus , Heijboer Annemieke C , Van den Ouweland Jody , Eisenhofer Graeme , Keevil Brian G , Rauh Manfred , Vogeser Michael , Pagotto Uberto

Background: LC-MS/MS is replacing immunoassays for serum steroid measurement in clinical labs worldwide. The intrinsic high specificity of LC-MS/MS is supposed to guarantee accurate quantitation, however, differences in pre-analytical and analytical procedures, and overall performance may influence the result. A few studies so far investigated the reproducibility among different LC-MS/MS assays for sex steroids. No data are available for adrenal steroids.<p class="abstext"...

ea0070aep14 | Adrenal and Cardiovascular Endocrinology | ECE2020

Comparability of steroid hormone measurement among 9 European laboratories using liquid chromatography-tandem mass spectrometry (LC-MS/MS): Impact of the blood derivative and of calibration

Fanelli Flaminia , Mezzullo Marco , Nardi Elena , Cantù Marco , Lindner Johanna M , Peitzsch Mirko , Hawley James M , Bruce Stephen J , Heijboer Annemieke C , Ackermans Mariette T , Van den Ouweland Jody , Koeppl Daniel , Eisenhofer Graeme , Rauh Manfred , Keevil Brian , Vogeser Michael , Pagotto Uberto

Background: LC-MS/MS is recommended for accurately measuring circulating steroids. However, laboratories using LC-MS/MS adopt various pre-analytical and analytical strategies, and little is known about comparability of results. Whether different blood derivatives impact LC-MS/MS measurements is unclear, and the benefit of unifying the calibration system among laboratories was not tested.Aim: Comparisons of LC-MS/MS measurements of circulating aldosterone...