Searchable abstracts of presentations at key conferences in endocrinology

ea0011p38 | Bone | ECE2006

Glucocorticoid metabolism in mouse osteoblasts: a model for the effects of local glucocorticoid generation on bone

Crook R , Hewitt K , Stewart PM , Rabbitt EH , Cooper MS

Human studies have suggested that local glucocorticoid (GC) generation within osteoblasts plays a critical role in bone loss seen during aging, in response to inflammation and treatment with GCs. Human osteoblasts express the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) that converts inactive GCs (cortisone, dehydrocorticosterone, prednisone) to their active counterparts (cortisol, corticosterone, prednisolone). Enzyme expression increases with age, in r...

ea0009p18 | Diabetes and metabolism | BES2005

Murine expression of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) and hexose-6-phosphate dehydrogenase (H6PDH)

Hewitt K , Bujalska I , Draper N , Onyimba C , Walker E , Stewart P

In humans, glucocorticoids (GC) are implicated in the pathogenesis of obesity and insulin resistance. GCs are regulated at the prereceptor level by 11beta-HSDs. 11beta-HSD1 predominately displays oxo-reductase activity (cortisone/11-dehydrocorticosterone to cortisol/ corticosterone), requiring the cofactor NADPH. Recently, studies in humans have shown that the enzyme H6PDH, by generating NAPDH in the endoplasmic reticulum (ER) is crucial for oxo-reductase activity. This study ...