Searchable abstracts of presentations at key conferences in endocrinology

ea0020p667 | Reproduction | ECE2009

Selected cytokines are associated with markers of insulin resistance in polycystic ovary syndrome

Stanicka Sona , Vrbikova Jana , Haluzik Martin , Hill Martin , Dvorakova Katerina , Grimmichova Tereza , Vondra Karel

The polycystic ovary syndrome (PCOS) is associated with features of the insulin resistance syndrome and altered glucose homeostasis. Factors that play an important role in these processes are still emerging. Pro-inflammatory cytokines may be involved in development of insulin resistance in PCOS. The purpose of this study was to determine if a relationship exists between interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), hepatocyte growth fa...

ea0020p496 | Obesity and Metabolism | ECE2009

Influence of finasteride treatment on metabolic profile of men with androgenetic alopecia

Duskova Michaela , Hill Martin , Starka Luboslav

Androgenetic alopecia, not only caused psychological distress, but also is the risk factor of cardiovascular diseases, glucose metabolism disorders, benign prostate hyperplasia and prostate carcinoma and suspected to present the sign of male equivalent of polycystic ovary syndrome. Finasteride, used for treatment of androgenetic alopecia in dose of 1 mg/day, is the first 5α-reductase type II inhibitor. The 5α-reductase is enzyme responsible for the reduction of testo...

ea0049ep208 | Steroid metabolism + action | ECE2017

Steroid metabolome changes in the 1 mg dexamethasone suppression test

Hana Vaclav , Kosak Mikulaš , Hana Vaclav , Hill Martin

Introduction: Dexamethasone induced negative feedback on CRH, ACTH and cortisol secretion is one of the key principles in the diagnostic workup of hypercortisolism. Cortisol secretion has been studied extensively, but changes of other steroids remained apart. Our aim was to establish steroid profile changes in 1 mg dexamethasone test.Methods: The Steroid profile consisting of 103 steroids and their metabolites from the serum of 9 male and 8 healthy femal...

ea0041ep42 | Adrenal cortex (to include Cushing's) | ECE2016

What can a tissue measured steroid metabolome tell about adrenal tumor? A tissue steroid analysis of cortisol producing adenoma and an androgen producing adenoma in a second patient with CAH, 21-hydroxylase deficiency

Hana Vaclav , Kosak Mikulas , Michalsky David , Hana Vaclav , Hill Martin

Introduction: A key feature of a benign adrenal tumor is its hormonal production. It is reflected by serum steroid levels. A tumoral tissue steroid content is not commonly measured.Methods: A steroid metabolome comprising of 65 steroids was measured in four samples from each of two patients using GC-MS in serum before and after 1mg dexamethasone test, in the tumoral tissue and in the adrenal tissue of two female patients with adrenal adenomas. The first ...

ea0037ep109 | Steroids, development and paediatric endocrinology | ECE2015

DHEA and its metabolites – daily profile

Sramkova Monika , Duskova Michaela , Starka Luboslav , Racz Beata , Hill Martin

Introduction: DHEA is a well-known neurosteroid. Its plasmatic level is reduced with ageing in most individuals. A non-negligible portion of DHEA is hydroxylated at C7 and C16 to 7α-, 7β- and 16α- and 16β-hydroxyderivatives. A part of the antiglucocorticoid function of DHEA has been described to its 7-oxygenated metabolites, namely to 7α-hydroxy-DHEA. Due its anti-glucocoticoid action it is also considered a factor in fat deposition processes. Thus, we...

ea0037ep119 | Steroids, development and paediatric endocrinology | ECE2015

Influence of food on daily profiles of steroids

Duskova Michaela , Racz Beata , Sramkova Monika , Hill Martin , Starka Luboslav

Introduction: Whereas the daily profiles of the main steroid hormones are well known, minor differences in the course of their levels related to defined and standardised food intake were studied rarely.Methods: Eight women (mean age 29.48±2.99 years, mean BMI 21.3±1.3 kg/m2) in follicular phase of menstrual cycle were examined. The levels of C-peptide, glucose, LH, FSH, SHBG, cortisol, testosterone, dihydrotestosterone, progesterone,...

ea0037ep619 | Obesity and cardiovascular endocrinology | ECE2015

Steroid hormones related to 11β-hydroxysteroid dehydrogenase in obese adolescents and non-obese controls

Macova Ludmila , Zamrazilova Hana , Sedlackova Barbora , Hill Martin

Introduction: Elevated activity and expression of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) are associated with the development of obesity and metabolic syndrome. This enzyme is responsible for majority of the extra-adrenal production of glucocorticoids. It regenerates active cortisol from biologically inactive cortisone. Human 11β-HSD1 also reversibly catalyzes the inter-conversion of 7α-hydroxy- and 7β-hydroxy-DHEA into 7-oxo-DHEA. In order to...

ea0070aep357 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Different steroidogenesis in patients with alzheimer’s disease and Type 2 diabetes mellitus

Vankova Marketa , Hill Martin , Vcelak Josef , Vejrazkova Daniela , Bendlova Bela

Alzheimer’s disease (AD) is a neurodegenerative diseasethat is manifested by a progressive loss of cognitive and behavioral function. In previous studies, we have constructed a predictive model for the classification of AD patients based on levels of circulating steroids and their polar conjugates.Both AD and Type 2 diabetes mellitus(T2DM) are well known to affect the levels of some steroid hormones, however, in the opposite direction, and in a gen...

ea0032p373 | Diabetes | ECE2013

Altered reactivity of pituitary–adrenal axis to stimulation tests and altered tissue metabolism of cortisol in long-standing type 1 Diabetes

Simunkova Katerina , Vondra Karel , Duskova Michaela , Hill Martin , Starka Luboslav

This work aimed to evaluate pituitary–adrenal axis and tissue metabolism of cortisol in patients with long standing type one diabetes.Fifty patients with diabetes type 1 were investigated; age 38±10 years (mean±S.D.), age at diagnosis 25.5±10 years, disease duration 15±8 years, BMI 24.5±2.7 kg/cm2, HbA1c 7.2±1.2%. Control group consisted of 40 healthy volunteers.The study...

ea0032p976 | Steroid metabolism and action | ECE2013

Changes of steroidogenesis subject to weight growth

Pospisilova Hana , Duskova Michaela , Hill Martin , Horak Luboslav

Introduction: Obesity epidemic has a considerable effect on the health of the population. Obesity is connected with infertility and hypogonadism. It is known that obese men have a low testosterone and higher estradiol level. The assumed mechanism would be increased testosterone aromatization in adipose tissue and a lower SHBG level, which means a decrease of overall testosterone. The aim of this study was to describe the changes of steroidogenesis with obese men.<p class="...