Searchable abstracts of presentations at key conferences in endocrinology

ea0022p820 | Thyroid | ECE2010

Rapid solid-phase-extraction for the quantitative analysis of serum iodothyronine profiles

Hoefig Carolin , Wohlgemuth Franziska , Klein Jeannette , Blankenstein Oliver , Schweizer Ulrich , Kohrle Josef

A novel solid-phase-extraction (SPE) method followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) was established for the simultaneous detection and quantification of thyroid hormone profiles in one sample of human or mouse serum.Recovery during preanalytical steps was monitored by addition of an internal deuterated standard (d5-T4) to 200 μl serum, which was deproteinized by adding a precipitating agent consisting of 1% f...