ea0084op-01-01 | Oral Session 1: Topic Highlights | ETA2022
Nunez Miguel Ricardo
, Sanders Paul
, Allen Lloyd
, Evans Michele
, Holly Matthew
, Johnson William
, Sullivan Andrew
, Miller-Gallacher Jennifer
, Sanders Jane
, Furmaniak Jadwiga
, Rees Smith Bernard
Objectives: The crystal structures of the TSH receptor (TSHR) leucine rich repeat domain (LRD) bound to TSHR stimulating monoclonal autoantibody M22TM or to TSHR blocking monoclonal autoantibody K1-70TM and antibody free have been solved previously. Cryo-electron microscopy (cryo-EM) was now used to solve the structure of full length TSHR in complex with K1-70TM.Methods: Recombinant human TSHR expressed in CHO cells was i...