Searchable abstracts of presentations at key conferences in endocrinology

ea0029p930 | Female Reproduction | ICEECE2012

The prevalence of endometrial hyperplasia and endometrial cancer in Danish women with PCOS or hyperandrogenism

Ravn P. , Glintborg D. , Andersen M. , Holm N. , Scledermann D.

Objective: PCOS may be associated with an increased risk of endometrial hyperplasia (EH) and endometrial cancer (EC), but substantial evidence remains to be established. We investigated the prevalence of EH and EC in a well-characterized group of women with PCOS and/or clinical/biochemical hyperandrogenism.Design: Retrospective, trans-sectional study.Setting: Out-patient clinic at the Departments of Endocrinology and Gynecology, Od...

ea0032p22 | Adrenal cortex | ECE2013

21-hydroxylase and interferon omega autoantibodies in Turner syndrome

Cleemann Line , Oftedal Bergithe , Trolle Christian , Holm Kirsten , Husebye Eystein , Gravholt Claus

Introduction: An increased frequency of autoimmune diseases and an elevated incidence of autoantibodies have been observed in Turner syndrome (TS), but indirect immunofluorescence (IIF) has not been able to demonstrate autoantibodies against the adrenal cortex. We asked if the more sensitive radioimmunosorbant assay employing recombinant human 21-hydroxylase was able to identify autoantibodies against 21-hydroxylase, (21OH-Ab) in TS patients; 21-hydroxylase is the major adrena...

ea0032p338 | Developmental Endocrinology | ECE2013

TurboFlow-LC-MS/MS method for quantification of DHEA, DHEAS, 17α-hydroxyprogesterone, Δ4-androstenedione and testosterone in children

Soeborg Tue , Frederiksen Hanne , Johannsen Trine Holm , Juul Anders , Andersson Anna-Maria

Diagnosis and management of infants and children with sex steroid disorders requires fast and simultaneous assessment of several sex steroid metabolites in serum at low concentrations and on small sample volumes. Therefore, we developed a sensitive and selective TurboFlow-LC-MS/MS method for quantification of DHEA, DHEAS, 17α-hydroxyprogesterone, Δ4-androstenedione and testosterone in serum from pre-pubertal children.Total run time was 10.75 mi...

ea0029p951 | Female Reproduction | ICEECE2012

Different effects of electrical and manual acupuncture stimulation on estrous cyclicity and neuroendocrine function in rats with DHT-induced polycystic ovary syndrome

Feng Y. , Johansson J. , Shao R. , Manneras Holm L. , Billig H. , Stener-Victorin E.

Both low-frequency electro-acupuncture (EA) and manual acupuncture improve menstrual frequency and decrease circulating androgens in women with polycystic ovary syndrome (PCOS). We sought to determine whether low-frequency EA is more effective than manual stimulation in regulating disturbed estrous cyclicity in rats with PCOS induced by 5α-dihydrotestosterone (DHT). To identify the central mechanisms of the effects of stimulation, we assessed hypothalamic mRNA expression ...

ea0014p197 | (1) | ECE2007

Neonatal sex steroid exposure of female rats results in insulin resistance and enlarged mesenteric adipocytes

Alexanderson Camilla , Lystig Theodore , Stener-Victorin Elisabeth , Lönn Malin , Holmäng Agneta

Introduction: Neonatal events may contribute to the development of disorders such as type 2 diabetes and obesity at adult age. We have previously shown that neonatal testosterone (T) programming of female rats is followed by insulin resistance and changes in adipose tissue distribution with centralization of body fat. Therefore, the aim of this study was to examine the effects of neonatal injection of T, estradiol (E) or dihydrotestosterone (DHT) on insulin sensitivity and siz...

ea0041ep25 | Adrenal cortex (to include Cushing's) | ECE2016

Low positive predictive value of midnight salivary cortisol measurement to detect hypercortisolism in type 2 diabetes

Steffensen Charlotte , Thomsen Henrik Holm , Dekkers Olaf M , Christiansen Jens S , Rungby Jorgen , Jorgensen Jens Otto L

Background: Hypercortisolism is prevalent in type 2 diabetes (T2D), but analytical and functional uncertainties prevail. Measurement of salivary cortisol is considered an expedient screening method for hypercortisolism, but its usefulness in the context of T2D is uncertain.Aim: To compare late night salivary cortisol (LNSC) with the 1 mg overnight dexamethasone suppression test (DST), which was considered ‘reference standard’, in T2D.<p cla...

ea0014p196 | (1) | ECE2007

Continuous administration of dihydrotestosterone or letrozole to immature female rats results in polycystic ovary syndrome characteristics at adult age

Mannerås Louise , Lystig Theodore , Holmäng Agneta , Ottosson-Lönn Malin , Stener-Victorin Elisabet

Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction, hyperandrogenism, abdominal obesity and insulin resistance. Since hyperandrogenism is a PCOS key feature, the aim was to evaluate the effects of androgen receptor activation in terms of continuously administration, beginning pre-pubertal, of either the non-aromatizable androgen dihydrotestosterone (DHT) or the aromatase inhibitor letrozole (L), on...

ea0032p792 | Paediatric endocrinology | ECE2013

Reference curves for body fat (%) for Danish children evaluated by skinfolds and dual-energy X-ray absorptiometry

Wohlfahrt-Veje Christine , Tinggaard Jeanette , Mouritsen Annette , Hagen Casper , Grunnet Mikkel , Tefre de Renzy-Martin Katrine , Boas Malene , Holm Pedersen Jorgen , M Main Katharina

Background: Over the last 60 years body composition of young people have changed towards increased fatness. Whole body fat percentage (%BF) derived from dual energy X-ray absorptiometry (DXA) scans are (although often not available) widely recognized as a better measure of fatness than BMI.Objective: We aimed to provide reference materials for %BF of healthy Danish children evaluated by skinfold measurements and DXA and to compare sensitivity and specifi...