Searchable abstracts of presentations at key conferences in endocrinology

ea0041ep158 | Calcium and Vitamin D metabolism | ECE2016

Investigation of interference with binding proteins in two commonly used 25-hydroxyvitamin D assays

Toldy Erzsebet , Viragh Eva , Horvath Dora Eszter , Kovacs Laszlo , Kovacs Eniko Andrea , Lakatos Peter , Locsei Zoltan

Background: Adequate vitamin D supply is necessary for bone metabolism and also for the general maintenance of health. 25OHD is transported primarily bound to vitamin-D binding protein (DBP), but also to albumin. Lower concentration of plasma proteins may cause difficulties in immunoanalytics. Our aim was to investigate the protein dependence of the two most often used 25OHD immuno- (CLIA) and protein binding- (ECLPBA) assays.Methods: levels were measure...

ea0022p61 | Adrenal | ECE2010

Comparison of plasma aldosterone/signal activity and aldosterone/active signal ratios in different clinical conditions

Locsei Zoltan , Toldy Erzsebet , Horvath Dora , Nagy Rita , Racz Karoly , Szabolcs Istvan , Kovacs Gabor L

Because measurement of plasma signal activity (PRA) is time-consuming an automated method directly measuring active signal (REN) may be more suitable for clinical practice. The aim of the study was to analyse the diagnostic utility of REN and aldosterone (ALD)/REN ratio as compared to PRA and ALD/PRA ratio.Materials and methods: PRA, REN (DiaSorin) and ALD (Immunotech) were simultaneously measured in 123 plasma samples (83 females and 40 males; mean age,...

ea0022p246 | Clinical case reports and clinical practice | ECE2010

Serum prolactin levels in rheumatoid arthritis and other autoimmune diseases

Toldy Erzsebet , Opra Barna , Horvath Dora , Locsei Zoltan , Kovacs Gabor L , Racz Karoly

In certain patients with autoimmune diseases (AID) and with rheumatoid arthritis (RA), moderate hyperprolactinemia can be present.Aim: Our aim was to investigate free PRL levels in RA, using two different PRL assays known to react with a different sensitivity to MPRL. The relationship between PRL and the disease-specific autoantibodies (antibody to cyclic citrullinated peptide:cCP-Ab and antinuclear antibody:AN-Ab) were analyzed also.<p class="abstex...

ea0022p833 | Thyroid | ECE2010

Is there a need to constitute laboratory- and/or region-specific reference ranges for the screening of thyroid disorders?

Horvath Dora , Toldy Erzsebet , Locsei Zoltan , Nagy Rita , Kovacs Gabor L , Szabolcs Istvan

Usually, the first laboratory marker for thyroid disorders is TSH. FT4 and FT3 must not be measured unless TSH is out of the reference range. However, the reference range for TSH (and for free thyroid hormones) given by the manufacturers of immunoassays may be not completely valid for screening in iodine-deficient areas and among hospitalized patients either.Aim: Our aim was to constitute specific TSH, FT4, FT3...

ea0032p114 | Calcium and Vitamin D metabolism | ECE2013

Vitamin D supply in healthy women with different reproductive stages: is there any relationship with DBP levels?

Toldy Erzsebet , Locsei Zoltan , Horvath Dora Eszter , Koppany Csaba , Kovacs Klaudia , Tancsics Emanuela , Kalman Bernadette , Racz Karoly

The total 25-hydoxy-vitamin-D (t-25OHD) level reflects the supply of vitamin D, but is also influenced by the levels of 25OHD binding proteins (DBP). We aimed studying t-25OHD and bioavailable 25OHD (bio-25OHD) levels in healthy females with different reproductive ages.Methods: t-25OHD, bio-intact parathormone (PTHi-1–84) (ECLIA, cobas e411, Roche), DBP (turbidimetry, Dako), calcium and albumin (Alb) levels were measured by routine methods (Modular,...