Searchable abstracts of presentations at key conferences in endocrinology

ea0012p78 | Pituitary | SFE2006

Upregulation of Id1 and Id3 in response to BMP2 stimulation in LβT2 cells

Hanson PL , Sidhu KK , Schwarz N , Milligan T , Burrin JM

The pathogenesis of gonadotrophin secreting pituitary tumours (gonadotrophinomas) remains poorly understood. Bone morphogenetic proteins (BMPs) are important for the control of cell proliferation and differentiation and BMP2 is essential for commitment of undifferentiated pituitary cells to the gonadotroph cell lineage. In addition, BMPs have been implicated in tumourigenesis in a variety of tissues, associated with up-regulated expression of Id (Inhibitors of differentiation)...

ea0011oc3 | Signal transduction OC1 Novartis Oncology Young Investigator Award | ECE2006

Activation of cAMP (cAMP) binding protein (CREB) by gonadotrophin-releasing hormone (GnRH) requires calcium influx and PKC activity in gonadotroph cell lines

Jonas KC , Thompson IA , Burrin JM , Fowkes RC

The ubiquitous transcription factor, CREB, is responsible for mediating gene transcription in many endocrine tissues. Recent studies reveal that the activation of several signalling pathways can lead to the phosphorylation of CREB at Ser133, including PKC, MAPKs, CaMKs, and PI3K. In the pituitary gonadotroph, there are consensus cAMP response elements (CREs) in several of the gonadotrophin subunit promoters as well as in the GnRH receptor promoter. We have previously shown tha...

ea0008oc20 | Young Endocrinologist Session | SFE2004

Sp1/Sp3, GnRH and PACAP involvement in C-type natriuretic peptide (CNP/Nppc) expression and action in gonadotrophs

Fowkes RC , Burrin JM , McArdle CA , Steinhelper ME

C-type natriuretic peptide (CNP) is encoded by the Nppcgene and is abundant in the hypothalamus and anterior pituitary gonadotrophs. Nppc deletion leads to skeletal deformity, dwarfism, early death and female infertility. To investigate regulation of Nppcexpression and action in the hypothalamic-pituitary-gonadal axis, we investigated transcriptional regulation of the Nppc promoter in alphaT3-1 gonadotroph cells by transient transfection of reporter...

ea0008p44 | Endocrine Tumours and Neoplasia | SFE2004

Expression of Bone Morphogenetic Protein receptors in human pituitary adenomas

Brown PL , Stoddart HL , Sidhu KK , Milligan TP , Burrin JM

Bone morphogenetic proteins (BMPs) regulate growth, differentiation and apoptosis in a variety of tissues and have crucial roles in the regulation of reproduction. BMPs bind specific Type II receptors and form complexes with Type I receptors (Alk2, 3 or 6) whereby the Type II receptors activate Type I receptors by phosphorylation. BMP2 and 4 play a crucial role in the development of the gonadotroph cell lineage during mouse pituitary morphogenesis and BMP6, 7, and 15 stimulate...

ea0012oc7 | Young Endocrinologist prize session | SFE2006

T3 stimulation of MAP kinase and cell proliferation in human osteoblast-like cells is initiated at integrin αVβ3

Scarlett AL , Parsons MP , Hanson PL , Sidhu KK , Milligan TP , Burrin JM

T3 and T4 rapidly activate intracellular signalling cascades via thyroid hormone receptor (TR)-independent actions, suggesting the existence of a plasma membrane receptor. Recent studies support a role for the RGD recognition site of integrin, a transmembrane glycoprotein, as a cell surface TR. We have demonstrated, using PCR and Western blotting, the expression of integrin αVβ3 mRNA and protein in the transformed human osteosarcoma cell line MG...

ea0019oc39 | Thyroid, reproduction and endocrine tumours | SFEBES2009

Contrasting clinical manifestations of SDH-B and VHL associated chromaffin tumours

Srirangalingam U , Khoo B , Walker L , MacDonald F , Skelly RH , George E , Spooner D , Johnston L , Monson JP , Grossman AB , Akker SA , Drake WM , Pollard PJ , Plowman N , Avril N , Berney DM , Burrin JM , Reznek RH , Kumar VKA , Maher ER , Chew SL

Background: Mutations in succinate dehydrogense-B (SDH-B) or von Hippel Lindau (VHL) genes can result in chromaffin tumours.Objective: To compare the clinical phenotypes of subjects developing chromaffin tumours as a result of SDH-B or VHL mutations.Subjects: Thirty-one subjects with chromaffin tumours. Sixteen subjects had SDH-B gene mutations and 15 subjects had a diagnosis of VHL.<p ...