Searchable abstracts of presentations at key conferences in endocrinology

ea0015p174 | Endocrine tumours and neoplasia | SFEBES2008

A new pathway in the induction of breast cancer

Watkins Rachel , Smith Vicki , Read Martin , Vasilopoulou Elisavet , James Sally , Boelaert Kristien , Franklyn Jayne , McCabe Christopher

Pituitary tumor transforming gene (PTTG) is a multifunctional oncogene implicated in the pathogenesis of pituitary and thyroid tumours. In breast cancer, PTTG expression is a prognostic marker for lymph node invasion and tumour recurrence. PTTG expression is regulated in vitro and in vivo by oestrogen treatment. PTTG binding factor (PBF) is also a transforming gene, and was isolated due to its functional interaction with PTTG. In the current study, we examined PB...

ea0015p178 | Endocrine tumours and neoplasia | SFEBES2008

A novel mechanism of NIS repression in differentiated thyroid cancer

Smith Vicki , Read Martin , Watkins Rachel , Turnell Andrew , James Sally , Boelaert Kristien , Franklyn Jayne , McCabe Christopher

The ability of the thyroid to accumulate iodide provides the basis for radioiodine ablation of differentiated thyroid cancers and their metastases. Most differentiated thyroid tumours exhibit reduced iodide uptake, however, and the mechanisms accounting for this remain poorly understood. Pituitary tumor transforming gene (PTTG) is a proto-oncogene implicated in the pathogenesis of thyroid tumours. We recently reported that PTTG and its binding factor PBF, also a potent transfo...

ea0013oc34 | British Thyroid Association Award | SFEBES2007

Monocarboxylate transporter 8 (MCT8) represses proliferation of fetal embryonal NT2 and placental JEG-3 cells in vitro

James Sally , McCabe Christopher , Reaves Barbara , Smith Vicki , Chan Shiao , Barrett Timothy , Kilby Mark , Franklyn Jayne

Thyroid hormones play vital roles in fetal brain development. Mutations in MCT8, recently recognised as a specific thyroid hormone transporter, define a novel syndrome of severe X-linked psychomotor retardation and thyroid hormone resistance.N-TERA-2 (NT2) cells (human embryonal cells with characteristics of CNS precursors) were transiently transfected with either WT MCT8 or its L471P, R271H or S448X mutations, described in males affected by severe psych...

ea0013p213 | AMEND Young Investigator's Award | SFEBES2007

The sodium iodide symporter (NIS) is repressed by PTTG via the human NIS upstream enhancer (hNUE)

Smith Vicki , Boelaert Kristien , Stratford Anna , James Sally , Kogai Takahiko , Franklyn Jayne , Mccabe Chris

The sodium iodide symporter (NIS) mediates the uptake of iodide into thyroid follicular cells. The pituitary tumor transforming gene (PTTG) is a multifunctional oncogene which stimulates expression of fibroblast growth factor-2 (FGF-2) via PTTG binding factor (PBF). PTTG and FGF-2 inhibit iodide uptake in rat thyroid FRTL5 cells and PTTG and PBF repress NIS mRNA expression and iodide uptake in primary human thyroid cultures. To determine whether this regulation is a direct tra...

ea0015p201 | Growth and development | SFEBES2008

Expression of thyroid hormone transporters in the human fetal cerebral cortex

Chan Shiao , Martin-Santos Azucena , Loubiere Laurence , Vasilopoulou Elisavet , James Sally , McCabe Chris , Franklyn Jayne , Kilby Mark

The importance of thyroid hormone (TH) transporters to central nervous system (CNS) development has been highlighted by reports of severe global neurological impairment associated with mutations in the potent TH transporter, MCT8. Even subclinical maternal hypothyroidism in early pregnancy has been associated with long-term neurodevelopmental delay in the offspring suggesting that TH is crucial for early fetal brain development. The cellular entry of TH requires the function o...