Searchable abstracts of presentations at key conferences in endocrinology

ea0032p928 | Pituitary – Clinical (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

Thyroid cancer in patients with acromegaly

Balcere Inga , Peculis Raitis , Klovins Janis , Pirags Valdis

Generously supported by IPSEN)-->It is known that acromegaly is associated with increased risk of benign and malignant tumours. Previous studies show the prevalence of nodular thyroid disease in 40–70% of patients with acromegaly, and a 5–7% prevalence of thyroid cancer in these patients, which is higher compared to the general population.Latvian Cancer Register data show an incidence of thyroid cance...

ea0014p257 | (1) | ECE2007

Rare polimorphism in the intron of human Agouti- related protein gene is associated with obesity

Kapa Ivo , Kalnina Ineta , Pirags Valdis , Schioth Helgi , Klovins Janis

Agouti related protein (AGRP) as a endogenous antagonist of melanocortin 4 receptor plays an important role in regulation of food intake and energy balance being one of the most potent orexigenic factors. We have determined complete sequence of AGRP gene and upstream promoter region in 100 patients with severe obesity (BMI>35). Three previously described polymorphisms were identified: silent mutation G538A in second exon, non-synonymous mutation G772A (rs5030980) and C662T...

ea0049oc6.3 | Diabetes therapy and complications | ECE2017

Alterations in DNA methylation from peripheral blood cells in humans threated with metformin

Klovins Janis , Elbere Ilze , Peculis Raitis , Kalnina Ineta , Zaharenko Linda , Konrade Ilze , Pirags Valdis

Metformin is a biguanide class agent widely used as a first-line treatment for type 2 diabetes, however the detailed mode of action and exact pharmacodynamics has not been clarified. Moreover there is no studies in humans displaying the effect of metformin on DNA methylation. We therefore performed a clinical study involving twelve healthy nondiabetic individuals who were subjected to the 1 week of metformin (2×850 mg/day) treatment. Peripheral blood for DNA isolation was...

ea0037ep522 | Diabetes (complications &amp; therapy) | ECE2015

Association of SNPs in the intergenic region of OCT2 and OCT3 with short-term efficiency of metformin monotherapy in the type 2 diabetes patients

Klovins Janis , Zaharenko Linda , Geldnere Kristine , Konrade Ilze , Grinberga Solveiga , Pugovics Osvalds , Kalnina Ineta , Pirags Valdis

Clinical response to metformin is highly variable in type 2 diabetes (T2D) patients highlighting the need for identification of genetic components affecting the efficiency of metformin therapy. Aim of this study was to evaluate the role of systematically selected tagSNPs from genomic regions coding for six organic cation transporters implicated in transport of metformin with respect to the short-term efficiency. The study involved 102 drug naïve newly diagnosed T2D patien...

ea0020p368 | Diabetes and Cardiovascular | ECE2009

Lack of association between polymorphisms at the adiponectin gene locus and type 2 diabetes as well as body mass index in Latvian population

Klovins Janis , Kalnina Ineta , Mackevics Vitolds , Fridmanis Davids , Nikitina-Zake Liene , Pirags Valdis

Association studies between genetic variants of adiponectin gene and susceptibility to type 2 diabetes (T2D) and increased body mass index (BMI) have provided contradictory results. We selected 10 SNPs (rs2241767, rs1501299, rs3777261, rs16861210, rs2241766, rs822396, rs182052, rs17300539, rs16861194, rs266729) in promoter and coding regions of adiponectin gene based on haploblock structure and previously reported association studies. Selected SNPs were screened in 170 patient...

ea0016p341 | Endocrine tumours | ECE2008

Association of genetic variants of somatostatin receptor 5 with acromegaly

Klovins Janis , Kapa Ivo , Balcere Inga , Ciganova Darja , Nikitina-Zake Liene , Schioth Helgi , Pirags Valdis

Full gene coding sequence of somatostatin receptor 5 and 2000 bp of upstream region was estimated using direct sequencing in 28 patients with acromegaly and 97 controls. Total 19 polymorphisms were identified (SNP1-19) and possible haplotypes were reconstructed. From all polymorphisms found silent substitution SNP15 was significantly associated with acromegaly as compared with control group (P=0.005). Another substitution SNP17 involves the amino acid change from prolin...

ea0049ep931 | Pituitary - Basic | ECE2017

Whole exome sequencing of two non-secreting pituitary adenoma tumors from the same patient

Rovite Vita , Peculis Raitis , Balcere Inga , Stukens Janis , Konrade Ilze , Valtere Andra , Nazarovs Jurijs , Caune Olivija , Pirags Valdis , Klovins Janis

Pituitary adenomas (PA) are benign tumors that develop in pituitary gland. Hormone secreting PA can cause overproduction of pituitary hormones leading to different systemic endocrine disorders (acromegaly, Cushing’s disease and others). Non-secreting PA can promote headaches (stretching of the dural sheath) and visual field defects (chiasma compression). We managed to obtain resected tumor samples from the patient having non-secreting PA, first tumor was resected in 2010,...

ea0063p52 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Exome analysis of pituitary adenoma tissue derived cell cultures

Rovite Vita , Peculis Raitis , Mandrika Ilona , Petrovska Ramona , Megnis Kaspars , Balcere Inga , Konrade Ilze , Stukens Janis , Breiksa Austra , Nazarovs Jurijs , Pirags Valdis , Klovins Janis

Introduction: Pituitary adenomas (PA) are tumours of the anterior pituitary. Despite the benign nature these neoplasms cause increased mortality and morbidity. Clinically relevant PAs affect around 0.1% of population during their lifetime. Currently, there is no human PA cell culture models. Tissue cultures derived from PA surgery materials depending on harvesting conditions can form free floating aggregates called pituispheres (PS) or adherent mesenchymal stromal cells (MSC)....