Searchable abstracts of presentations at key conferences in endocrinology

ea0049oc13.3 | Reproduction & Endocrine Disruption | ECE2017

Sertoli cell expressed hydroxysteroid (17beta) dehydrogenase 1 is required for male fertility

Hakkarainen Janne , Jokela Heli , Zhang Fuping , Kotaja Noora , Sipila Petra , Poutanen Matti

: Hydroxysteroid (17beta) dehydrogenase 1 (Hsd17b1) is a steroidogenic enzyme catalyzing the conversion of estrone (E1) to estradiol (E2), and androstenedione (A-dione) to testosterone (T). We have shown that the deletion of Hsd17b1 gene in mice resulted in the failure of ovarian estrogen production and subfertility. In this study, we clarified the role of Hsd17b1 in male reproduction. The data revealed that in mice Hsd17b1 mRNA is highly expressed in the Sertoli cells at the ...

ea0049ep364 | Cardiovascular Endocrinology and Lipid Metabolism | ECE2017

Deleting genomic region of Hsd17b1 in mice results into a hypomorphic Naglu allele, and consequently to a phenotype mimicking a lysosomal storage disease

Jokela Heli , Hakkarainen Janne , Pakarinen Pirjo , Ruohonen Suvi , Zhang Fuping , Poutanen Matti

Hydroxysteroid 17-beta dehydrogenase 1 (HSD17B1) is an enzyme catalyzing the reduction of estrone (E1) to estradiol (E2), as well as androstenedione (Adione) to testosterone (T). To elucidate the physiological function of HSD17B1, we generated knockout mice with disrupted Hsd17b1 gene using targeted ES cells (clone 10231) obtained from KOMP repository ( In these ES cells the whole coding region of Hsd17b1 was replaced with LacZ/Neo cass...

ea0022p258 | Developmental endocrinology | ECE2010

De novo cholesterol synthesis in developing mouse embryo is required for embryonic survival

Jokela Heli , Rantakari Pia , Lamminen Tarja , Strauss Leena , Ola Roxana , Gylling Helena , Miettinen Tatu , Pakarinen Pirjo , Sainio Kirsi , Poutanen Matti

Hydroxysteroid (17-β) dehydrogenase enzymes (HSD17Bs) have an important role in sex steroid hormone metabolism. They are known to catalyze reactions between highly active 17β-hydroxy steroids and less active 17-keto steroids. In addition to sex steroid metabolism, it is becoming evident that HSD17Bs have functions in other pathways as well, e.g. lipid and bile acid metabolism. HSD17B type 7 enzyme is known to catalyze in vitro the activation of estrone (E1) to...