Searchable abstracts of presentations at key conferences in endocrinology

ea0063gp100 | Adrenal and Neuroendocrine - Basic | ECE2019

Characterization of cell death induced by mitotane in adrenocortical carcinoma cells

Weigand Isabel , Schreiner Jochen , Rohrig Florian , Kiseljak-Vassiliades Katja , Hofner Kerstin , Kendl Sabine , Fassnacht Martin , Sbiera Silviu , Kroiss Matthias

Background: Mitotane is the only drug approved for treatment of adrenocortical carcinoma (ACC). We have found that mitotane leads to endoplasmic reticulum stress and decreased viability in ACC cells. It is not known by which downstream mechanisms cell death is induced by mitotane.Aim: To characterize the mechanisms underlying cell death resulting from mitotane treatment in ACC cells.Methods: Lipid peroxidation in the ACC cell line ...

ea0070oc7.5 | Endocrine-related Cancer | ECE2020

Differential adrenal toxicity of SOAT1-inhibitors

Weigand Isabel , Sbiera Silviu , Kendl Sabine , Hanna Urlaub , Constanze Hantel , Katja Kiseljak-Vassiliades , Margaret E Wierman , Fassnacht Martin , Kroiss Matthias

Background: Mitotane is the only approved treatment for advanced adrenocortical carcinoma and was shown to inhibit Sterol-O-Acyl transferase 1 (SOAT1) which leads to the depletion of cholesterol esters and increase of free cholesterol in the ACC cell line H295R. Downstream activation of the endoplasmic reticulum stress (ER-stress) pathway results in decreased adrenocortical cell viability.Aim: To better characterize the effects of SOAT1 inhibition in ACC...

ea0070aep96 | Adrenal and Cardiovascular Endocrinology | ECE2020

Confirmatory testing of primary aldosteronism with saline infusion test and LC-MS/MS

Teresa Fuss Carmina , Brohm Katharina , Kurlbaum Max , Hannemann Anke , Kendl Sabine , Fassnacht Martin , Deutschbein Timo , Hahner Stefanie , Kroiss Matthias

Objective: The saline infusion test (SIT) is a standard confirmatory test for primary aldosteronism (PA) and based on impaired aldosterone suppression in PA compared to essential hypertension (EH). In the past, aldosterone was quantified using immunoassays (IA). Due to the more widespread use of liquid chromatography tandem mass spectrometry (LC-MS/MS) in clinical routine, we aimed at a method-specific aldosterone threshold for the diagnosis of PA during SIT and explored the d...

ea0070aep5 | Adrenal and Cardiovascular Endocrinology | ECE2020

Cytochrome P450 (CYP) 2W1 affect steroid secretion in adrenocortical cell line and tumor tissues

Altieri Barbara , Sbiera Silviu , Weigand Isabel , Volante Marco , Steinhauer Sonja , Lorey Antonia , Kendl Sabine , Kurlbaum Max , Herterich Sabine , Della Casa Silvia , Colao Annamaria , Terzolo Massimo , Kroiss Matthias , Fassnacht Martin , Ronchi Cristina

Background: The human cytochrome P450 (CYP) 2W1 is involved in the metabolism of several endogenous substrates, including lysophospholipids and fatty acids. Using a polyclonal antibody, we previously demonstrated a high CYP2W1 immunoreactivity in adrenocortical tumors, particularly in those secreting steroids. The aim of the study was to better elucidate the relationship between CYP2W1 and steroid secretion in adrenocortical carcinoma (ACC) H295R cell line and in a larger seri...

ea0037gp.29.01 | Endocrine tumours and neoplasia – Adrenal Tumour | ECE2015

Mitotane inhibits sterol-o-acyltransferase leading to lipid-mediated endoplasmic reticulum stress and apoptosis of adrenocortical carcinoma cells

Sbiera Silviu , Fassnacht Martin , Leich Ellen , Liebisch Gerhard , Schirbel Andreas , Wiemer Laura , Matysik Silke , Vanselow Jens T , Gardill Felix , Gehl Annemarie , Kendl Sabine , Beyer Melanie , Bala Margarita , Ronchi Cristina L , Deutschbein Timo , Schlosser Andreas , Schmitz Gerd , Rosenwald Andreas , Allolio Bruno , Kroiss Matthias

Context: Mitotane is the only drug approved for treatment of adrenocortical carcinoma (ACC) and in clinical use for more than 50 years. Mitotane counteracts both tumour growth and tumoral steroid hormone production but treatment is associated with severe side effects. The molecular mechanism of mitotane is still unknown, which hampers progress in treatment of ACC.Objective: To identify the mechanism of action and molecular target of mitotane.<p class...