Searchable abstracts of presentations at key conferences in endocrinology

ea0028p136 | Growth and development | SFEBES2012

Maturational effects of glucocorticoids on fetal cardiomyocytes are direct and mediated by glucocorticoid receptor

Rog-Zielinska Eva , Kenyon Chris , Holmes Megan , Chapman Karen

In addition to their essential role in fetal lung development, glucocorticoids promote late-gestation maturation of the fetal heart as shown by severe functional impairment, structural disorganisation and transcriptome immaturity in fetal hearts of glucocorticoid receptor-deficient (GR-/-) mice. Here we use primary fetal mouse cardiomyocytes (E16-E17.5) to investigate whether these effects of glucocorticoids result from direct actions of GR and whether they are independent of ...

ea0021p341 | Steroids | SFEBES2009

Moderate liquorice consumption enhances salivary DHEA and testosterone levels in healthy volunteers

Al-Dujaili Emad A S , Macdonald Claire , Kenyon Chris

Liquorice root has been used medicinally for many years to treat dry cough, asthma, hunger, thirst, sterility of women, fever, digestive problems and pathologies of organs such as lungs, stomach, intestines and kidney. Glycyrrhetinic acid (one of the active constituents in liquorice) has diverse in vitro effects as an inhibitor of 11β hydroxysteroid dehydrogenase (11HSD), 5α reductase and hormone receptor binding. The aim of this study is to investigate the ef...

ea0013p169 | Diabetes, metabolism and cardiovascular | SFEBES2007

Are there multiple endogenous glucocorticoids? Chronic Effects of 5α-Tetrahydrocorticosterone in C57BL/6 Mice

Yang Chenjing , Livingstone Dawn , Walker Brian , Kenyon Chris , Andrew Ruth

The rate limiting steps of hepatic glucocorticoid clearance are via A-ring reduction by 5α- and 5β-reductases. Our recent in vitro studies suggested that products of 5α-reduction (eg 5α-tetrahydrocorticosterone) can activate glucocorticoid receptors and hence may contribute to glucocorticoid action in liver. Here we compared the effects of administering 5α-tetrahydrocorticosterone (5αTHB) in vivo with those of corticosterone (B)....

ea0038p383 | Steroids | SFEBES2015

Adrenocortical function in glucocorticoid receptor deficient mice

Mohammadi-Zaniani Ghazaleh , Richardson Rachel , Morley Steve , Chapman Karen , Kenyon Chris

Introduction: Humans with glucocorticoid receptor (GR) deficiency and global heterozygous GR knockout (GR+/−) show compensatory activation of the hypothalamic-pituitary-adrenal axis, resulting in salt-sensitive hypertension due to increased mineralocorticoid activity. Previous studies suggest renal mechanisms, including changes in cell proliferation, gene expression and electrolyte transport, may contribute to this phenotype but underlying adaptive adrenal responses have...

ea0028oc4.6 | Steroid | SFEBES2012

Fatty liver in a mouse model of congenital adrenal hyperplasia is driven by peroxisome proliferator-activated receptors

McInnes Kerry , Gallacher Stuart , Caughey Sarah , Carter Rod , Yau Joyce , Mullins Linda , Kenyon Chris

Mice with congenital adrenal hyperplasia due to a null mutation of cyp11b1, the gene encoding the steroidogenic enzyme 11β-hydroxylase, have low glucocorticoid levels, reduced adiposity and increased liver weight. It is notable that null mice are also glucose intolerant. Here, we have investigated these phenotypes in more detail. Plasma corticosterone levels were less responsive to restraint stress and cholesterol and triglyceride levels were not significantly different i...

ea0016p264 | Endocrine disruptors | ECE2008

Investigating the effects of Liquorice consumption on Salivary Steroid hormones profile and blood pressure in healthy volunteers

Al-Dujaili Emad A S , Macdonald Claire , Kenyon Chris J , Nicol Moira

Glycyrrhetinic acid (GA) has diverse in vitro effects as an inhibitor of 11beta hydroxysteroid dehydrogenase (11HSD), 5alpha reductase and hormone receptor binding. However, in vivo GA studies have focussed on the hypertensive effects associated with the syndrome of apparent mineralocorticoid excess (SAME) in which 11HSD inhibition allows glucocorticoid hormones to bind inappropriately to MR and subsequent decreased aldosterone synthesis. Here we consider whether...