Searchable abstracts of presentations at key conferences in endocrinology

ea0044oc4.3 | Adrenal and Steroids | SFEBES2016

Genome wide ChIP-Seq analysis of Glucocorticoid Receptor and RNA Polymerase 2 binding in rat liver during physiological and non-physiological corticosterone replacement

Flynn Benjamin P. , Birnie Matthew T. , Kershaw Yvonne M. , Baek Songjoon , Kim Sohyoung , Stavreva Diana A. , Hager Gordon L. , Lightman Stafford L. , Conway-Campbell Becky L.

Ultradian glucocorticoid (GC) secretion is highly conserved, having been detected in all species studied. The GC corticosterone (CORT) is a ligand for the glucocorticoid receptor (GR), inducing GR recruitment to glucocorticoid responsive elements (GREs) to modulate transcription of GC-target genes. We have previously demonstrated that pulsatile GR recruitment to GREs upstream of the Period1 gene is associated with its pulsatile transcription in rat liver. Similarly th...

ea0038p385 | Steroids | SFEBES2015

Genome-wide binding analysis of glucocorticoid receptors in the rat hippocampus in response to corticosterone and stress

Flynn Benjamin P , Pooley John R , Kershaw Yvonne M , Baek Songjoon , Grontved Lars , Guertin Michael J , Hager Gordon L , Lightman Stafford L , Conway-Campbell Becky L

Glucocorticoids act via the glucocorticoid receptor (GR). Upon ligand binding, GR translocates to the nucleus and binds directly to glucocorticoid responsive elements (GREs) to regulate transcriptional output. Glucocorticoid secretion increases in response to stress to affect transcriptional output within specific areas of the brain including the hippocampus (HC). Therefore, here we assess changes in genome-wide GR chromatin binding profiles in the rat HC in response to a gluc...