Searchable abstracts of presentations at key conferences in endocrinology

ea0035p1081 | Thyroid (non-cancer) | ECE2014

Involvement of Lat2 in the transport of 3,3′-T2 across the plasma membrane and first structural insights into transport mechanisms by homology model generation

Kinne Anita , Hinz Katrin , Wittner Melanie , Krause Gerd

Thyroid hormones (THs) are transported into their target cells by diverse transmembrane transporter proteins, e.g. by members of the L-type amino acid transporter (LAT) family. The Lat2 is a sodium independent amino acid exchanger and interacts with CD98 for its efficient cell surface expression. So far the role of Lat2 in TH transport including the transport mechanisms is unclear. It is important to determine TH subtype specificity and to identify amino acids at Lat2 which ar...

ea0020p573 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Transport activities and plasma membrane localization of MCT8 mutant proteins identified in patients with severe psychomotor retardation depend on cell type. Implications for the interpretation of clinical phenotypes

Kinne Anita , Roth Stephan , Schweizer Ulrich , Kohrle Joseph

Objective: Mutations in the gene encoding the thyroid hormone transport protein, monocarboxylate transporter 8 (MCT8), underlie severe mental retardation. In vitro expression of mutant transporters was performed to understand phenotypical differences.Methods: We established cell lines stably expressing 16 MCT8 variants in JEG1 and MDCK cells. Several of these mutants have never been analysed before. The cell lines were characterized according to M...