Searchable abstracts of presentations at key conferences in endocrinology

ea0016p522 | Obesity | ECE2008

Glucose-dependent insulinotropic polypeptide downregulates expression and enzyme activity of 11β-Hydroxysteroid Dehydrogenase type 1

Andres Janin , Gogebakan Ozlem , Mai Knut , Pfeiffer Andreas , Spranger Joachim

Introduction: 11β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1) converts cortisone into its active metabolite, cortisol. Mechanisms regulating the promoter-activity of 11β-HSD1 are of considerable importance to understand the basics of intracellular cortisol, lipid and glucose metabolism. The incretine glucose-dependent insulinotropic polypeptide (GIP) has been suggested to affect insulin sensitivity. However, the mechanisms of this effect are unclear yet....

ea0020p464 | Obesity and Metabolism | ECE2009

Glucose-dependent insulinotropic polypeptide increases glucose-uptake in muscle- and adipocyte cells in vitro

Andres Janin , Biedasek Katrin , Goegebakan Ozlem , Mai Knut , Pfeiffer Andreas , Spranger Joachim

Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by K-cells of proximal small intestine that is secreted postprandial in response to glucose and fat ingestion. Its effect is mediated through a GIP receptor (GIPR) which is widely expressed in different tissues, e.g. pancreatic islets, adipose tissue, skeletal muscle, adrenal cortex, heart, pituitary and in some regions of the brain. However, only the effect of GIP in pancreatic beta-cells is we...

ea0020p584 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Feedback inhibition of prednisolone on vasopressin (AVP) secretion but preserved renal water reabsorption after thirsting point to an AVP independent antidiuretic action

Ufer Friederike , Mai Knut , Diederich Sven , Pedersen Erling , Pfeiffer Andreas , Bahr Volker

Adrenal insufficiency can result in severe hyponatremia due to inappropriate high plasma vasopressin (pAVP). To elucidate the glucocorticoid AVP feedback we monitored thirsting of 12 male volunteers without and after on or five days of prednisolone (30 mg/d). Although prednisolone suppressed pAVP below 0.2 pg/ml the rise in plasma osmolality during thirsting was not influenced by prednisolone. Independent of exogenous glucocorticoid thirsting resulted in higher urine osmolalit...

ea0016p215 | Diabetes and cardiovascular diseases | ECE2008

Metabolic changes induced by glucose infusion in persons with Glu23Lys polymorphism

Bobbert Thomas , Mittag Marc , Mai Knut , Fischer Antje , Arafat Ayman , Pfeiffer AFH , Spranger Joachim

The development of diabetes mellitus type 2 (T2DM) is affected by genetic and lifestyle factors. Polymorphisms within the KIR6.2 gene seem to be associated with a higher risk for T2DM. The protein encoded by this gene represents a potassium channel, which plays an essential role in insulin and glucagon secretion by the pancreas.A hyperglycaemic clamp without insulin was performed over 2 h in 10 healthy persons with the Kir6.2 E23K wild type Glu/Glu, 10 a...

ea0081oc10.2 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Success in implementing changes in macronutrients intake in a high-protein and high-unsaturated fatty acids dietary intervention: 36-months results of the NutriAct randomized controlled multi-center trial

Pletsch-Borba Laura , Wernicke Charlotte , Meyer Nina , Nguyen Thu , Pohrt Anne , Gerbracht Christiana , Pfeiffer Andreas , Spranger Joachim , Mai Knut

Background & Aims: NutriAct is a 36-month randomized controlled multi-center trial aiming to analyze the effects of a dietary pattern focusing on a high-protein and high-unsaturated fatty acids (UFA) intake on healthy aging. We aimed to explore changes in intake of macronutrients and determine factors associated with a successful modulation of dietary pattern after 36 months in elderly community dwelling participants.Methods: 502 participants were ra...

ea0016p23 | Adrenal | ECE2008

Effects of free fatty acids on adrenocorticotropin secretion and metabolic glucocorticoid pattern in healthy young women

Mai Knut , Reinecke Franziska , Bobbert Thomas , Andres Janin , Bahr Volker , Wudy Stefan , Hartmann Michaela , Maser-Gluth Christiane , Diederich Sven , Pfeiffer Andreas , Spranger Joachim

Introduction: Free fatty acids (FFAs) affect pituitary function. While no effect of FFAs on ACTH and cortisol seems to exist in men, data in women are somewhat controversial. Therefore, we aimed to assess the effect of an acute increase of circulating FFA levels induced by the infusion of a lipid-heparin infusion on ACTH and cortisol secretion in healthy young women.Methods: Following a 10-h overnight fast 13 healthy female volunteers were investigated i...

ea0073aep183 | Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Increase in PUFA and in protein intake leads to reduction of liver fat independently of weight loss in 12 months: the NutriAct trial

Pletsch-Borba Laura , Wernicke Charlotte , Apostoloupoulou Konstantina , Spira Dominik , Pohrt Anne , Hornemann Silke , Gerbracht Christiana , Pfeiffer Andreas , Spranger Joachim , Mai Knut

BackgroundLong-term effects of an increase in unsaturated fatty acids and protein on changes in intrahepatocellular lipids (IHL) are still unclear. We aimed to explore the impact of changes in individual macronutrients on IHL changes within the NutriAct intervention trial over 12 months in middle-aged and elderly subjects (50–80 yrs) at risk for age-related diseases.DesignIn the NutriAct randomized contr...

ea0016p22 | Adrenal | ECE2008

Free fatty acids affect urinary excretion of androgen precursors thereby linking metabolism and hyperandrogenemia in vivo: results of a randomized, controlled trial

Mai Knut , Bobbert Thomas , Reinecke Franziska , Andres Janin , Bahr Volker , Maser-Gluth Christiane , Wudy Stefan , Hartmann Michaela , Mohlig Matthias , Weickert Martin O , Schulte Heinrich , Diederich Sven , Pfeiffer Andreas , Spranger Joachim

Context: The polycystic ovarian syndrome (PCOS) is characterized by hyperandrogenism and associated with obesity and impaired glucose metabolism. Despite the high prevalence of PCOS and the considerable clinical impact, the precise interplay between metabolism and hyperandrogenemia is not entirely clear. We therefore aimed to analyse the relation between circulating free fatty acids (FFAs) and androgen metabolism in vivo in women.Design and partic...