Searchable abstracts of presentations at key conferences in endocrinology

ea0049ep781 | Endocrine Disruptors | ECE2017

Characterization of murine Leydig cell lines as tools to study androgen synthesis disruption by xenobiotics

Engeli Roger , Furstenberger Cornelia , Kratschmar Denise , Odermatt Alex

Mammalian Leydig cells produce the majority of the systemic levels of the primary male sex hormone testosterone. Testosterone plays a crucial role during development of male reproductive tissues, onset of puberty, and maintaining health state. The final step of testosterone synthesis is catalyzed by 17β-hydroxysteroid dehydrogenase 3 (17β-HSD3). Disruption of testosterone synthesis is associated with many diseases. Due to the lack of a human Leydig cell line, two dif...

ea0049ep822 | Steroid metabolism + action | ECE2017

Inhibition of 11β-hydroxysteroid dehydrogenase 2 by the fungicides itraconazole and posaconazole

Beck Katharina , Kratschmar Denise , Meyer Arne , Schuster Daniela , Odermatt Alex

Impaired 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2)-dependent cortisol inactivation can lead to electrolyte dysbalance, hypertension and cardiometabolic disease. Furthermore, placental 11β-HSD2 essentially protects the fetus from high maternal glucocorticoid levels, and its impaired function has been associated with altered fetal growth and a higher risk for cardio-metabolic diseases in later life. Despite its important role, 11β-HSD2 is not include...

ea0049ep782 | Endocrine Disruptors | ECE2017

Identification of chemicals disrupting adrenal steroid production by steroid profiling in H295R cells

Strajhar Petra , Tonoli David , Jeanneret Fabienne , Imhof Raphaella , Malagnino Vanessa , Patt Melanie , Kratschmar Denise , Boccard Julien , Rudaz Serge , Odermatt Alex

Human adrenal H295R cells are applied according to the validated OECD test guideline 456 to identify potential endocrine disrupting chemicals. Testosterone and estradiol production serves as read-out although these are not steroids typically produced by the adrenals. The current study attempted to optimize conditions for using H295R cells to detect chemicals disturbing the synthesis of key adrenal steroids. Culture supernatants of H295R cells were analysed by LC-MS-based stero...