Searchable abstracts of presentations at key conferences in endocrinology

ea0014p121 | (1) | ECE2007

Analysis of BRAF point mutation in papillary thyroid carcinoma

Sykorova Vlasta , Dvorakova Sarka , Laco Jan , Ryska Ales , Kodetova Daniela , Astl Jaromir , Vesely David , Bendlova Bela

BRAF point mutations are found in 29-69% of papillary thyroid carcinoma (PTC). BRAF is a serine-threonine kinase involved in the phoshorylation of MAPK signaling pathway. The mutation is located in the exon 15 of BRAF, resulting in the substitution of valine to glutamate at codon 600 (V600E). Mutation generates unregulated B-Raf activity that leads to increased cellular proliferation. The aim of this study was to determine the frequency of BRAF mutation in the Czech population...

ea0032p834 | Pituitary–Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2013

No correlation between somatostatine 2 receptor expression analyzed by RNA in-situ hybridization and real-time qRT-PCR in clinically non-functioning pituitary adenomas

Gabalec Filip , Kohout Ales , Laco Jan , Drastikova Monika , Beranek Martin , Netuka David , Masopust Vaclav , Cesak Tomas , Cap Jan

Objective: The aim of this study was to quantitatively estimate SSTR2 in clinically non-functioning pituitary adenomas (CNFAs) with use of RNA in-situ hybridization (ISH) and quantitative real-time RT-PCR and correlate the results of both methods.Methods: A standard histological and immunohistochemical examination was performed on the resected pituitary tumour. Afterwards in-situ hybridization for somatostatine 2 receptor (SSTR2) RNA wa...