Searchable abstracts of presentations at key conferences in endocrinology

ea0026oc1.5 | Thyroid/Adrenal | ECE2011

Adrenal crisis and general morbidity in chronic adrenal insufficiency prospectively assessed in 472 patients

Hahner Stefanie , Spinnler Christina , Beuschlein Felix , Fassnacht Martin , Lang Katharina , Quinkler Marcus , Allolio Bruno

Adrenal crisis (AC) is a life threatening condition in chronic adrenal insufficiency (AI). Furthermore, recent data demonstrate increased mortality in this patient group.Incidence and causes of adrenal crisis as well as general morbidity and hospitalization was assessed in 472 patients in a prospective trial. Patients were contacted via mail every 6 months and in case of AC additionally by phone to evaluate the exact circumstances of AC. Criteria for AC ...

ea0020p20 | Adrenal | ECE2009

Chemokine receptor expression in the adrenal cortex and in adrenocortical tumours

Lang Katharina , Sturmer Andrea , Adam Patrick , Fassnacht Martin , Quinkler Marcus , Morcos Michael , Allolio Bruno , Hahner Stefanie

Introduction: Chemokines and their receptors (CR) have been demonstrated to be involved in tumour growth and site specific metastasis. Furthermore, several cytokines have been described to modulate adrenocortical function. Therefore, we have investigated the expression pattern and functional activity of chemokine receptors and of corresponding chemokines in adrenocortical tissue.Methods: Chemokine and CR expression was assessed by RT-PCR in normal adrena...

ea0016oc4.4 | Bone and adrenal | ECE2008

Comparison of different test systems for the screening of inhibitors of CYP11B enzymes

Stuermer Andrea , Hahner Stafanie , Lang Katharina , Linss Heidi , Zink Martina , Schirbel Andreas , Allolio Bruno

Subject: Inhibitors of CYP11B enzymes, particularly of aldosterone synthase, are currently under development as adrenostatic agents in heart failure or hyperaldosteronism, and for use in adrenal imaging. As CYP11B1 and CYP11B2 show high homology, analysis of specific binding to the respective human enzymes is of key importance.Methods: Y1 cell lines stably expressing hsCYP11B1 or hsCYP11B2 were established. Primary cultures of porcine and human adrenocor...

ea0049gp122 | Endocrine Tumours | ECE2017

The natural history of adrenal incidentaloma – results from the international prospective multi-centre EURINE-ACT study

Bancos Irina , Chortis Vasileios , Lang Katharina , Prete Alessandro , Terzolo Massimo , Fassnacht Martin , Quinkler Marcus , Kastelan Darko , Vassiliadi Dimitra , Beuschlein Felix , Ambroziak Urszula , Sitch Alice , Deeks Jonathan , Arlt Wiebke

Background: Adrenal masses are discovered in 5% of abdominal imaging scans. Work-up aims at exclusion of malignancy and hormone excess. However, estimates of these risks derive from retrospective studies only, mostly small and with significant selection bias.Methods: Prospective multi-centre study (2011–2016) in 14 centres (11 countries) of the European Network for the Study of Adrenal Tumours (ENSAT) with prospective consecutive enrolment of patien...

ea0044oc4.6 | Adrenal and Steroids | SFEBES2016

Demographics of adrenal incidentaloma – results from an international prospective multi-centre study in 2190 patients

Bancos Irina , Chortis Vasileios , Lang Katharina , Terzolo Massimo , Fassnacht Martin , Quinkler Marcus , Kastelan Darko , Vassiliadi Dimitra , Beuschlein Felix , Ambroziak Urszula , Deeks Jonathan , Arlt Wiebke

Background: Adrenal masses are discovered in 5% of cross-sectional abdominal imaging scans. Work-up aims at exclusion of malignancy and hormone excess. However, estimates of these risks presently derive from retrospective studies only, mostly small and with significant selection bias.Methods: Prospective multi-centre study (2011–2016) in 21 centres (17 countries) of the European Network for the Study of Adrenal Tumours (ENSAT) with consecutive enrol...

ea0038oc2.3 | Translational pathophysiology and therapeutics | SFEBES2015

Further advances in diagnosis of adrenal cancer: a high-throughput urinary steroid profiling method using liquid chromatography tandem mass spectrometry (LC-MS/MS)

Taylor Angela , Bancos Irina , Chortis Vasileios , Lang Katharina , O'Neil Donna , Hughes Beverly , Jenkinson Carl , Deeks Jon , Shackleton Cedric , Biehl Michael , Arlt Wiebke

Context: Differentiating adrenocortical adenoma (ACA) from adrenocortical carcinoma (ACC) represents a continuous challenge in patients with (often incidentally discovered) adrenal masses, with unfavorable sensitivities and specificities provided by tumor size, imaging and even histology. We have previously developed urine steroid metabolomics as a tool for the detection of adrenal malignancy employing gas chromatography mass spectrometry (GC-MS) for the detection of 32 distin...

ea0037ep77 | Adrenal cortex | ECE2015

Prevalence of neoplasms in patients with primary aldosteronism

Lang Katharina , Weber Katrin , Quinkler Marcus , Dietz Anna , Wallaschofski Henri , Hannemann Anke , Vonend Oliver , Willenberg Holger , Reincke Martin , Allolio Bruno , Hahner Stefanie

Context: Primary aldosteronism (PA) is the most common cause of secondary hypertension. Aldosterone excess can cause oxidative stress and respectively DNA damage in vitro and in vivo and increased levels of oxidative stress have been demonstrated in PA patients. Single case reports describe a coincidence of PA with renal cell carcinoma (RCC) and other tumours. However, so far no data on the prevalence of benign and malignant neoplasms in patients with PA exis...

ea0038oc1.6 | Early Career Oral Communications | SFEBES2015

Urine steroid metabolomics as a diagnostic tool in primary aldosteronism

Lang Katharina , Beuschlein Felix , Biehl Michael , Dietz Anna , Riester Anna , Hughes Beverly A , O'Neil Donna M , Hahner Stefanie , Quinkler Marcus , Lenders Jacques W , Shackleton Cedric H L , Reincke Martin , Arlt Wiebke

Introduction: The regular diagnostic workup for primary aldosteronism (PA) can be very demanding and involves multiple invasive as well as time and cost intensive diagnostic tests. Here we have explored the value of urinary steroid metabolome analysis in the diagnosis and differential diagnosis of PA. Previously, urinary 3α,5β-tetrahydroaldosterone (THAldo) has been suggested as a reliable screening test for PA and serum 18-oxocortisol and 18-hydroxycortisol have bee...

ea0038oc3.4 | Steroids and adrenal | SFEBES2015

Urine steroid metabolomics as a novel diagnostic tool for early detection of recurrence in adrenocortical carcinoma

Chortis Vasileios , Bancos Irina , Lang Katharina , Hughes Beverly , O'Neil Donna , Taylor Angela , Fassnacht Martin , Bertherat Jerome , Beuschlein Felix , Quinkler Marcus , Vassiliadi Dimitra , Dennedy M Conall , Mannelli Massimo , Biehl Michael , Arlt Wiebke

Introduction: Adrenocortical carcinoma (ACC) is an aggressive malignancy with a high rate of recurrence. Regular post-operative follow-up imaging is necessary, but associated with high radiation exposure and frequent diagnostic ambiguity. Urine steroid metabolomics has recently been introduced as a novel diagnostic tool for the detection of adrenocortical malignancy in patients with adrenal incidentalomas. Here we present the first clinical study assessing the performance of t...

ea0022oc4.1 | Adrenals | ECE2010

Subtype differentiation in primary hyperaldosteronism: evaluation of alternative algorithms avoiding a controversial gold standard

Lang Katharina , Hahner Stefanie , Vonend O , Rump L C , Quninkler M , Diederich Sven , Maier Katharina , Beuschlein Felix , Bidlingmaier Martin , Endres S , Engelke C , Kickuth Ralph , Fassnacht Martin , Reincke Martin , Allolio Bruno

Context: Primary aldosteronism (PHA) is the most common cause of secondary hypertension. Distinguishing unilateral (UAH) from bilateral (BAH) and other causes of PHA is one of the greatest challenges in the diagnostic process. Adrenal venous sampling (AVS) is widely considered to be the gold standard for subtype differentiation.Methods: Technical and diagnostic outcome of AVS was analysed retrospectively in 59 patients with PHA who underwent AVS at the U...