Searchable abstracts of presentations at key conferences in endocrinology

ea0065p184 | Metabolism and Obesity | SFEBES2019

Vitamin B12 deficiency leads to fatty acid metabolism dysregulation and increased pro-inflammatory cytokine production in human adipocytes and in maternal subcutaneous and omental adipose tissue

Samavat Jinous , Adaikalakoteswari Antonysunil , Boachie Joseph , Jackisch Laura , McTernan Philip , Christian Mark , Saravanan Ponnusamy

Vitamin B12 (B12) is an essential micronutrient required for several metabolic reactions. Animal and clinical studies show that B12-deficiency is associated with metabolic syndrome. Given the key metabolic role of adipose tissue, we investigated whether B12 deficiency may affect triglyceride synthesis and lipid metabolism leading to adipose tissue inflammation. The AbdSc pre-adipocyte cell line (Chub-S7) and human AbdSc primary pre-adipocytes were differentiated under differen...

ea0065p185 | Metabolism and Obesity | SFEBES2019

Endotoxin and adiposity as mediators of down-regulating the BRITE fat phenotype

Omran Farah , Murphy Alice , Jackisch Laura , Samavat Jinus , McTernan Philip G , Christian Mark

Background: The acquisition of brown-adipocyte-properties by white-adipocytes (BRITE-adipocytes) is an appealing-prospect to combat obesity and type-2-diabetes-Mellitus (T2DM). This may support counteracting the impact of inflammation and mitochondrial-dysfunction, which contribute to the obesity-pathogenesis. However, our previous-finding shave shown that the gut-derived-inflammatory-agent endotoxin can increase the inflammatory-response in white-adipose-tissue impacted by ob...

ea0065p202 | Metabolism and Obesity | SFEBES2019

Endoplasmic reticulum stress directly impacts mitochondrial function in human adipocytes

Jackisch Laura , Murphy Alice , Kumar Sudhesh , Randeva Harpal , Tripathi Gyanendra , McTernan Philip

Background: Dysfunctional endoplasmic reticula (ER) and mitochondria contribute to the pathogenesis of obesity and type 2 diabetes mellitus (T2DM). This may, in part, be facilitated by cross-talk between the two organelles during conditions of nutrient excess such as obesity, however the potential impact of ER stress on mitochondrial function has not been well studied. This study investigated whether induction of ER stress in human adipocytes may contribute to mitochondrial dy...

ea0044p179 | Obesity and Metabolism | SFEBES2016

Metabolic endotoxaemia impairs mitochondrial respiration and insulin sensitivity in human adipocytes

de la Escalera Lucia Martinez , Jackisch Laura , Murphy Alice , Piya Milan , Kumar Sudhesh , Tripathi Gyanendra , McTernan Philip G

Background: Metabolic endotoxaemia (raised bacterial endotoxin in serum after high-fat feeding) has been shown to reduce insulin sensitivity in humans through systemic inflammation and oxidative stress. Mitochondria represent the main source of cellular reactive oxygen species and mutations in mitochondrial DNA often result in a diabetic phenotype. However, the direct cellular impact of endotoxin on mitochondrial respiration and DNA integrity, particularly within the context o...

ea0044p193 | Obesity and Metabolism | SFEBES2016

Tunicamycin-induced ER stress mediates mitochondrial dysfunction in human adipocytes

Jackisch Laura , de la Escalera Lucia Martinez , Murphy Alice , Al-Daghri Nasser , McTernan Philip , Randeva Harpal , Tripathi Gyanendra

Background: The pathogenesis of obesity and T2DM mediates mitochondrial dysfunction which, in part, may arise as a consequence of endoplasmic reticulum (ER) stress. However, the potential impact of ER stress on mitochondria dysfunction is unclear. Therefore, we investigated whether induction of ER stress contributes to mitochondrial dysfunction in human adipocytes using 1) human differentiated adipocyte cell line (Chub-S7, n=12); and 2) primary differentiated lean and...

ea0044p191 | Obesity and Metabolism | SFEBES2016

Freeze dried broccoli extract relieves ER stress and mitochondrial inefficiency in differentiated human pre-adipocyte cells

Murphy Alice , de la Escalera Clapp Lucia Martinez , Jackisch Laura , Azharian Sahar , Aladel Alanoud , Barker Guy , Tripathi Gyanendra , Chappell Michael , McTernan Philip

Background: In obesity, excess nutrients can disrupt protein folding in the endoplasmic reticulum (ER) which activates the unfolded protein response (UPR) and alters mitochondrial function. These changes can induce inflammation, oxidative stress and insulin resistance. The aim of the study was to investigate whether broccoli extract can protect against cellular damage in human adipocytes, which with mathematical modelling may help predict pathway response.<p class="abstext...

ea0038p199 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Evidence for improved mitochondrial efficiency in adipose tissue of T2DM women after malabsorptive but not restrictive bariatric surgery

Martinez de la Escalera Lucia , Jackisch Laura , Kyrou Ioannis , Vrbikova Jana , Hainer Vojtech , Fried Martin , Sramkova Petra , Piya Milan , Kumar Sudhesh , Tripathi Gyanendra , McTernan Philip

Aims/hypothesis: Genetic and functional defects in mitochondria are linked with phenotypes of obesity, hypercholesterolemia, and type 2 diabetes (T2DM). However, the impact on mitochondria during surgery-induced metabolic recovery is unknown. We therefore studied the effect of restrictive versus malabsorptive bariatric procedures on indicators of mitochondrial efficiency and control of biogenesis/function.Methods: Forty-three morbidly obese, T2DM consent...