Searchable abstracts of presentations at key conferences in endocrinology

ea0034p332 | Reproduction | SFEBES2014

Regulation of the LH/CG receptor signalling in human endometrium

West Camilla , Hanyaloglu Aylin , Brosens Jan , Lavery Stuart , Panay Nick

It is well established that the interaction between an implanting embryo and the endometrium is essential for successful implantation and there is a variety of locally secreted factors within the uterine environment which underlies this maternal–fetal cross-talk. Human chorionic gonadotrophin (hCG) is a glycoprotein hormone secreted by the embryo and is essential during early stages of pregnancy to maintain progesterone production from the corpus luteum. Its receptor (LH/...

ea0025p276 | Reproduction | SFEBES2011

Defining insulin signalling pathways in granulosa-lutein (GL) cells in women with polycystic ovary syndrome (PCOS)

Joharatnam Jalini , Lamanna Giuseppina , Trew Geoffrey , Lavery Stuart , Hardy Kate , Franks Stephen

Aim: PCOS is associated with peripheral insulin resistance and we have previously shown that glucose uptake and metabolism are impaired in granulosa lutein (GL) cells of anovulatory women with PCOS (anovPCO). The aim of this study was to delineate insulin-signalling pathways in GL cells from women with anovPCO and to compare the response to insulin with that in GL cells from ovulatory women with (ovPCO) and without polycystic ovaries (controls).Methods: ...

ea0056gp130 | Female Reproduction | ECE2018

Alterations in gonadotropin receptors and signal activation in granulosa lutein cells from women with polycystic ovary syndrome

Owens Lisa , Christopolous Georgios , Khanjani Shirin , Hardy Kate , Lavery Stuart , Franks Stephen , Hanyaloglu Aylin

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age. The underlying pathogenesis is complex and incompletely understood. There is evidence that gonadotropin and gonadotropin receptor action play a role in the pathogenesis of PCOS with genome wide association studies in PCOS also implicating altered gonadotropin action in the aetiology of PCOS. Furthermore, our recent studies have demonstrated that gonad...

ea0094p64 | Metabolism, Obesity and Diabetes | SFEBES2023

Female AKR1D1 knockout mice are protected against diet induced obesity and insulin resistance but not hepatic steatosis

Bailey Maira , Potter Tom , Larner Dean , Morgan Stuart , Lavery Gareth , Tomlinson Jeremy , Gathercole Laura

Bile acids and steroid hormones are potent regulators of metabolic phenotype. 5β-reductase (AKR1D1) is highly expressed in the liver where it catalyses a fundamental step in bile acid synthesis and inactivates steroid hormones. We have previously shown that male, but not female, AKR1D1 knockout (KO) mice on a normal chow diet are leaner than wildtype littermates but are not protected against diet induced obesity. Here we investigate the impact of a high fat diet on female...

ea0038p399 | Steroids | SFEBES2015

Glucocorticoid-induced lipolysis across human ageing and the relationship to fat mass and androgens

Hassan-Smith Zaki , Taylor Angela , Hughes Beverly , Brady Theresa , Jones Pamela , Morgan Stuart , Lavery Gareth , Tomlinson Jeremy , Stewart Paul

Background: Excess glucocorticoid (GC) exposure is associated with an adverse body composition and metabolic profile characterised by increasing fat mass, muscle atrophy, insulin resistance, fatty liver, and dyslipidaemia culminating in increased cardiovascular risk. GCs are widely prescribed with increasing age where their effects can have deleterious effects.Objectives: To investigate the impact of GC exposure on subcutaneous adipose lipid metabolism a...

ea0044p211 | Reproduction | SFEBES2016

Insight into the molecular mechanisms underlying alterations in gonadotropin receptor activity in polycystic ovarian syndrome

Owens Lisa , Lerner Avi , Sposini Silvia , Christopoulos George , Khanjani Shiran , Islam Razia , Lavery Stuart , Tsui Vicky , Hardy Kate , Franks Stephen , Hanyaloglu Aylin

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age, and is the major cause of anovulatory infertility. Aberrant secretion and/or action of gonadotropins are implicated but, to date, we have only limited knowledge about the precise mechanisms involved. Recent genome wide association studies have discovered signals at loci close to the genes coding for gonadotropin receptors. The functional significanc...

ea0056oc2.1 | Look who is controlling your gonads! | ECE2018

The in vivo and in vitro effects of kisspeptin on human ovarian function

Owens Lisa , Abbara Ali , Lerner Avi , O'Floinn Shannon , Christopoulos Georgios , Khanjani Shirin , Islam Rumana , Liyanage Maneshka , Hardy Kate , Lavery Stuart , Hanyaloglu Aylin , Dhillo Waljit , Franks Stephen

Background: Infertility is a common problem and the number of couples receiving assisted reproductive treatment (ART) is increasing. Using GnRH agonists (GnRHa) to trigger oocyte maturation has been shown to reduce risk of ovarian hyperstimulation syndrome (OHSS), a common complication of IVF, compared to the more traditional use of human chorionic gonadotropin (hCG). Kisspeptin has recently been shown to be a safe trigger of oocyte maturation in women at high risk of OHSS. Ki...

ea0044oc6.1 | Pregnancy and Reproductive Health | SFEBES2016

Two doses of kisspeptin improve oocyte maturation and implantation rates compared to a single kisspeptin injection during IVF treatment

Abbara Ali , Clarke Sophie , Islam Rumana , Prague Julia , Comninos Alexander , Narayanaswamy Shakunthala , Papadopolou Deborah , Roberts Rachel , Nesbitt Alexander , Izzi-Engbeaya Chioma , Vimalesvaran Sunitha , Ratnasabapathy Risheka , Salim Rehan , Lavery Stuart , Bloom Stephen , Trew Geoffrey , Dhillo Waljit

Background: In vitro fertilisation is an effective therapy for infertility, but can result in the potentially life-threatening complication, Ovarian Hyper-Stimulation Syndrome (OHSS). We have previously reported that a single injection of kisspeptin results in an LH surge of ~12–14 hrs duration, sufficient to safely induce oocyte maturation, but eliminate OHSS. However, the physiological LH surge in a normal menstrual cycle has a plateaux lasting 24–28 hrs. ...